Abstract:
:The argH gene encoding argininosuccinate lyase (ASL) of Methanococcus maripaludis was cloned on a 4.7-kb HindIII genomic fragment. The gene is preceded by a short open reading frame (ORF149), which encodes a polypeptide with an unknown function. The two genes are co-transcribed. The ASL of M. maripaludis shares a high amino acid identity with ASLs from both bacterial and eukaryal origins and was able to complement both an argH Escherichia coli mutant and an arg4 yeast mutant, showing its extraordinary evolutionary conservation. Attempts to create an argH auxotroph of M. maripaludis by disrupting the genomic allele were unsuccessful: although a knockout allele of argH was integrated into the M. maripaludis chromosome by homologous recombination, the intact copy was not excluded, suggesting that the argH gene is essential.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Cohen-Kupiec R,Kupiec M,Sandbeck K,Leigh JAdoi
10.1111/j.1574-6968.1999.tb13507.xkeywords:
subject
Has Abstractpub_date
1999-04-01 00:00:00pages
231-8issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(99)00081-6journal_volume
173pub_type
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