Construction and properties of pyruvate dehydrogenase complexes with up to nine lipoyl domains per lipoate acetyltransferase chain.

abstract：:To investigate the function of the pro-peptide (PP) region of the Staphylococcus hyicus exolipase, restriction sites were created in the lipase gene to facilitate the construction of deletions in this region. Lipase gene expression was carried out in Staphylococcus carnosus. In the presence of the entire PP region, th...

journal_title：FEMS microbiology letters

authors： Demleitner G,Götz F

更新日期：1994-08-15 00:00:00

abstract：:Endoglucanase of Aspergillus niger AS-101 was partially purified by ammonium sulphate fractionation and molecular sieving on Sephadex G-200. The enzyme was found to be unstable on storage, however, it received some protection on the addition of BSA, glycerol or sodium azide. Glycerol also protected the enzyme from ina...

journal_title：FEMS microbiology letters

authors： Singh A,Agrawal AK,Abidi AB,Darmwal NS

更新日期：1990-09-01 00:00:00

abstract：:DNA sequence analysis of the nadA gene of Ehrlichia chaffeensis revealed a 942 bp open reading frame with the capacity to encode 314 amino acids. The amino acid sequence of the E. chaffeensis quinolinate synthetase A (NAD/A) has 53.6% identity and 82% similarity to the NAD A of the cyanelle of Cyanophora paradoxa. Por...

journal_title：FEMS microbiology letters

authors： Yu XJ,Walker DH

更新日期：1997-09-01 00:00:00

abstract：:Competition assays measure differences between populations of bacteria after stress adaptation, populations of different bacteria and mutations in antibiotic resistance genes. We have developed a competition-based assay to evaluate if genes upregulated under starvation are important for bacterial survival. Stress resp...

journal_title：FEMS microbiology letters

authors： Havis S,Rangel J,Mali S,Bodunrin A,Housammy Z,Zimmerer R,Murphy J,Widger WR,Bark SJ

更新日期：2019-03-01 00:00:00

abstract：:The 16S ribosomal RNA gene sequences of Lactococcus lactis ssp. lactis and ssp. cremoris differ by 9-10 bp (depending on strain), within the first 200 bp of the sequence. These differences were used to develop two methods of genetically differentiating lactis and cremoris strains. Primers to conserved sequences in the...

journal_title：FEMS microbiology letters

authors： Ward LJ,Brown JC,Davey GP

更新日期：1998-09-01 00:00:00

abstract：:Mycobacterium leprae, an obligate intracellular pathogen, shows a unique tropism for Schwann cells (SC). This leads to the peripheral neuropathy disorder observed in leprosy. In this study, we investigated signal transduction events and the intracellular fate of M. leprae during the interaction of the microorganism wi...

journal_title：FEMS microbiology letters

authors： Alves L,de Mendonça Lima L,da Silva Maeda E,Carvalho L,Holy J,Sarno EN,Pessolani MC,Barker LP

更新日期：2004-09-15 00:00:00

abstract：:The plant-growth promoting rhizobacterium Azospirillum lipoferum strain 4B generates in vitro a stable phase variant designated 4VI at frequencies of 10(-4) to 10(-3) per cell per generation. Variant 4VI displays pleitropic modifications, such as the loss of swimming motility and the inability to assimilate certain su...

journal_title：FEMS microbiology letters

authors： Vial L,Pothier JF,Normand P,Moënne-Loccoz Y,Bally R,Wisniewski-Dyé F

更新日期：2004-07-15 00:00:00

abstract：:The genes mphA and mphB encode macrolide 2'-phosphotransferases I and II, respectively, and they confer resistance to macrolide antibiotics in Escherichia coli. To study the expression of these genes in Gram-positive bacteria, we constructed recombinant plasmids that consisted of an mph gene and the pUB110 vector in B...

journal_title：FEMS microbiology letters

authors： Noguchi N,Tamura Y,Katayama J,Narui K

更新日期：1998-02-15 00:00:00

abstract：:A Phoma sp. was isolated and characterized as endophytic and as a pathogen of Larrea tridentata (creosote bush) growing in the desert region of southern Utah, USA. This fungus produces a unique mixture of volatile organic compounds (VOCs), including a series of sesquiterpenoids, some alcohols and several reduced napht...

journal_title：FEMS microbiology letters

authors： Strobel G,Singh SK,Riyaz-Ul-Hassan S,Mitchell AM,Geary B,Sears J

更新日期：2011-07-01 00:00:00

abstract：:The development of a methodology to identify the origin of fecal pollution is important both for assessing the degree of risk posed to public health and for developing strategies to mitigate the environmental loading of pathogens associated with waterborne disease transmission. Five rep-PCR genomic fingerprinting meth...

journal_title：FEMS microbiology letters

authors： Mohapatra BR,Broersma K,Mazumder A

更新日期：2007-12-01 00:00:00

abstract：:Fungi of the genus Fusarium are important plant pathogens and contaminants of cereal grains producing different types of mycotoxins. Enniatins are a group of mycotoxins with ionophoric properties frequently detected in North European grains. Within the Fusarium complex responsible for grain infection, Fusarium avenace...

journal_title：FEMS microbiology letters

authors： Kulik T,Jestoi M,Okorski A

更新日期：2011-01-01 00:00:00

abstract：:Rhizobium leguminosarum bv. trifolii rosR gene encodes a transcriptional regulator involved in the positive regulation of exopolysaccharide synthesis. Transcription of rosR is directed by two promoters, distal P1 and proximal P2, of different strengths. We demonstrated that rosR P1 functions as the main promoter and, ...

journal_title：FEMS microbiology letters

authors： Janczarek M,Skorupska A

更新日期：2009-02-01 00:00:00

abstract：:To determine the existence of an acid stress response in Helicobacter pylori the global changes in the proteins synthesized by the bacterium when subjected to an acid stress were studied. H. pylori ATCC43504 previously adapted to pH 7 did not show an acid stress response as detected by the two-dimensional electrophore...

journal_title：FEMS microbiology letters

authors： Toledo H,Valenzuela M,Rivas A,Jerez CA

更新日期：2002-07-16 00:00:00

abstract：:Enterococcus faecium has emerged as an important cause of nosocomial infections over the last two decades. We recently demonstrated collagen type I (CI) as a common adherence target for some E. faecium isolates and a significant correlation was found to exist between acm-mediated CI adherence and clinical origin. Here...

journal_title：FEMS microbiology letters

authors： Zhao M,Sillanpää J,Nallapareddy SR,Murray BE

更新日期：2009-11-01 00:00:00

abstract：:PhoP was previously shown to be important for Yersinia pestis survival in macrophage and under macrophage-induced stresses. In this work, a phoP disruptant of Y. pestis 201 was generated using the Red cloning procedure. The transcription profile of the wild-type Y. pestis was then compared with that of the phoP mutant...

journal_title：FEMS microbiology letters

authors： Zhou D,Han Y,Qin L,Chen Z,Qiu J,Song Y,Li B,Wang J,Guo Z,Du Z,Wang X,Yang R

更新日期：2005-09-01 00:00:00

abstract：:Recent molecular approaches for the study of microbial communities such as PCR-cloning have enabled the detection and identification of as-yet-unculturable taxa. Cloning and sequencing of multiple samples is extremely laborious and expensive to perform thoroughly due to the large diversity involved. For this purpose, ...

journal_title：FEMS microbiology letters

authors： Gafan GP,Spratt DA

更新日期：2005-12-15 00:00:00

abstract：:Within the phylum Bacteroidetes, the gyrB gene, encoding for the B subunit of the DNA gyrase, has been used as a phylogenetic marker for several genera closely related to Flavobacterium. The phylogenies of the complete 16S rRNA gene and the gyrB gene were compared for 33 Antarctic Flavobacterium isolates and 23 type s...

journal_title：FEMS microbiology letters

authors： Peeters K,Willems A

更新日期：2011-08-01 00:00:00

abstract：:The study was made of the antibacterial effects of three terpene alcohols on Staphylococcus aureus, focusing on the leakage of K+ ions and toxicity over time. The leakage of K+ ions was monitored continuously with a K+-electrode. Our results suggested that the terpene alcohols, namely, farnesol, nerolidol and plaunoto...

journal_title：FEMS microbiology letters

authors： Inoue Y,Shiraishi A,Hada T,Hirose K,Hamashima H,Shimada J

更新日期：2004-08-15 00:00:00

abstract：:Staphylococcus simulans biovar staphylolyticus secreted two bacteriolytic peptidoglycan hydrolases as proproteins that were activated as they were processed by an extracellular sulphydryl protease. This processing resulted in the production of multiple molecular-mass forms of each enzyme. Cells from early exponential ...

journal_title：FEMS microbiology letters

authors： Neumann VC,Heath HE,LeBlanc PA,Sloan GL

更新日期：1993-06-15 00:00:00

abstract：:A gene encoding a phosphomannose isomerase from Streptococcus mutans GS-5 was identified immediately downstream from the fructokinase gene, scrK. Nucleotide sequence analysis of this region revealed an open reading frame (ORF) specifying a putative protein of 316 amino acids. The gene cloned in Escherichia coli expres...

journal_title：FEMS microbiology letters

authors： Sato Y,Yamamoto Y,Kizaki H,Kuramitsu HK

更新日期：1993-11-15 00:00:00

abstract：:We developed a multiplex PCR to detect the presence of methicillin- (mecA), cadmium/zinc-(czrC) and antiseptic-resistant (qacA/B) staphylococci and to identify Panton-Valentine leukocidin (PVL)-positive and -negative Staphylococcus aureus and coagulase-negative staphylococci (CoNS) from infected and healthy eyes. The ...

journal_title：FEMS microbiology letters

authors： Panda S,Kar S,Choudhury R,Sharma S,Singh DV

更新日期：2014-03-01 00:00:00

abstract：:Bifidobacteria are normal inhabitants of the human gut, and members of which are generally considered to be probiotic. Before exerting their beneficial properties, they must survive and persist in the physiological concentrations (0.05-2%) of bile in the gut. In this work, the functional role of tlyC1 encoding a hemol...

journal_title：FEMS microbiology letters

authors： Liu Y,An H,Zhang J,Zhou H,Ren F,Hao Y

更新日期：2014-11-01 00:00:00

abstract：:We found the presence of DNA sequence which shows sequence similarity to the class IV chitin synthase gene (CHS3) of Saccharomyces cerevisiae in the genome of 14 Rhizopus species which belong to zygomycetes. We cloned a gene (chs3), which might correspond to one of these homologous sequences, from Rhizopus oligosporus...

journal_title：FEMS microbiology letters

authors： Motoyama T,Horiuchi H,Ohta A,Yamaguchi I,Takagi M

更新日期：1998-12-01 00:00:00

abstract：:The P39 antigen is a specific, highly conserved, and immunogenic protein of Lyme disease spirochetes, Borrelia burgdorferi sensu lato. The nucleotide sequence of the gene encoding this protein was determined and found to be the first of two tandemly arranged open reading frames located on the spirochete's chromosome. ...

journal_title：FEMS microbiology letters

authors： Simpson WJ,Cieplak W,Schrumpf ME,Barbour AG,Schwan TG

更新日期：1994-06-15 00:00:00

abstract：:An accurate monitoring of disease progression is important to evaluate disease susceptibility phenotypes. Over the years, Arabidopsis thaliana has become the model species to serve as a host in plant-pathogen interactions. Despite the efforts to study genetic mechanisms of host defense, little efforts are made for a t...

journal_title：FEMS microbiology letters

authors： Brouwer M,Lievens B,Van Hemelrijck W,Van den Ackerveken G,Cammue BP,Thomma BP

更新日期：2003-11-21 00:00:00

abstract：:In this multidisciplinary study, we combined morphological, physiological, and phylogenetic approaches to identify three dominant water bloom-forming Cyanobacteria in a tropical marine mangrove in Guadeloupe (French West Indies). Phylogenetic analysis based on 16S rRNA gene sequences place these marine Cyanobacteria i...

journal_title：FEMS microbiology letters

authors： Guidi-Rontani C,Jean MR,Gonzalez-Rizzo S,Bolte-Kluge S,Gros O

更新日期：2014-10-01 00:00:00

abstract：:When Escherichia coli STII was applied to the serosa of the ileum at a concentration of 40 micrograms/ml, an acceleration of the spontaneous motility and a weak contraction were induced 2-3 min later. The induction was not affected by the addition of atropine (10(-6) M), but was abolished by the addition of papaverine...

journal_title：FEMS microbiology letters

authors： Hitotsubashi S,Akagi M,Saitou A,Yamanaka H,Fujii Y,Okamoto K

更新日期：1992-01-15 00:00:00

abstract：:The presence of insertion sequence IS1 in 70 multiple-antibiotic resistant clinical strains was determined. This 70-strain collection comprised 46 Escherichia coli, 18 Salmonella and 6 Shigella strains. The presence of IS1 was detected in the chromosome and plasmids of 73% and 63% of the strains, respectively, and 51%...

journal_title：FEMS microbiology letters

authors： Ramirez-Santos J,Alvarez G,Cisneros E,Gomez-Eichelmann MC

更新日期：1992-06-01 00:00:00

abstract：:Bacteria communicate with one another and with their host using chemical signalling molecules. This phenomenon is generally described as quorum sensing. A set of primers for PCR detection of Salmonella spp. has been designed using as target the sdiA gene which encodes a signal receptor of the LuxR family. The PCR prod...

journal_title：FEMS microbiology letters

authors： Halatsi K,Oikonomou I,Lambiri M,Mandilara G,Vatopoulos A,Kyriacou A

更新日期：2006-06-01 00:00:00

abstract：:A Gram-negative sporulating thermophilic anaerobe, designated AB11Ad, was isolated from the heated waters of the Great Artesian Basin of Australia. It grew on a variety of carbohydrates including glucose, starch, and dextran and produced a thermostable and thermoactive extracellular endo-dextranase. The enzyme was pro...

journal_title：FEMS microbiology letters

authors： Wynter C,Patel BK,Bain P,de Jersey J,Hamilton S,Inkerman PA

更新日期：1996-07-01 00:00:00