Abstract:
:Halobacillus trueperi accumulates glycine betaine under condition of high osmolarity. A fragment of the glycine betaine transporter betH gene was obtained from the genome of H. trueperi with degenerate primers. Through Southern blot hybridization and inverse PCR, a 5.1 kb EcoRI fragment containing the complete betH gene was identified and subsequently sequenced. The betH gene was predicted to encode a 55.2 kDa protein (504 amino acid residues) with 12 transmembrane regions. BetH showed 56% identity to the OpuD of Bacillus subtilis which belongs to the betaine/carnitine/choline transporter (BCCT) family. Its putative promoter region was highly homologous to sigmaB-dependent promoter of B. subtilis. A 2.6 kb fragment containing the betH gene was cloned into pUC18 and transformed into the Escherichia coli MKH13. The accumulation of glycine betaine in transformed E. coli MKH13 bacteria was confirmed using 13C nuclear magnetic resonance spectroscopy.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Lu W,Zhao B,Feng D,Yang Sdoi
10.1016/j.femsle.2004.05.015keywords:
subject
Has Abstractpub_date
2004-06-15 00:00:00pages
393-9issue
2eissn
0378-1097issn
1574-6968pii
S0378109704003532journal_volume
235pub_type
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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更新日期:1998-08-01 00:00:00
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