Respiration of [14C]alanine by the ectomycorrhizal fungus Paxillus involutus.

Abstract:

:The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [14C]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [14C]alanine was further actively metabolised to glutamate, glutamine and aspartate. Exposure to aminooxyacetate completely suppressed 14CO2 evolution and greatly reduced the flow of carbon from [14C]alanine to tricarboxylic acid cycle intermediates and amino acids, suggesting that alanine aminotransferase plays a pivotal role in alanine metabolism in Paxillus involutus.

journal_name

FEMS Microbiol Lett

authors

Chalot M,Brun A,Finlay RD,Söderström B

doi

10.1111/j.1574-6968.1994.tb07080.x

subject

Has Abstract

pub_date

1994-08-01 00:00:00

pages

87-91

issue

1

eissn

0378-1097

issn

1574-6968

journal_volume

121

pub_type

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