Functional interactions between homologous conditional killer systems of plasmid and chromosomal origin.

Abstract:

:parD and chpA are homologous conditional killer systems of plasmid and chromosomal origin, respectively, encoding a killer protein (Kid and ChpAK) and an antidote (Kis and ChpAI). Here it is shown that these systems can functionally interact. A multicopy chpA recombinant partially complements two mutations in the antidote of the parD system. These mutations affect either autoregulation or neutralization of the killer component. Following in vitro mutagenesis with hydroxylamine, chpA mutants that improve this complementation were isolated. Sequence analysis shows that these mutants are clustered in the 5' end of the chpAI gene and structure predictions suggest that they affect a putative loop in the secondary structure of the ChpAI antidote. It is proposed that this region is part of a protein-protein interface required for the functional interaction between the antidote and the killer components in the two homologous systems.

journal_name

FEMS Microbiol Lett

authors

Santos-Sierra S,Giraldo R,Díaz-Orejas R

doi

10.1111/j.1574-6968.1997.tb10408.x

subject

Has Abstract

pub_date

1997-07-01 00:00:00

pages

51-6

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(97)00177-8

journal_volume

152

pub_type

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