A class II hydrophobin gene, Trhfb3, participates in fungal asexual development of Trichoderma reesei.

abstract：:Molecular microbial ecology studies are heavily reliant on 'Universal' 16S rRNA gene primers for elucidating microbial community structure and composition, and yet primer design and optimization is often overlooked. Primers that exhibit minor biases due to primer-template mismatches can substantially alter the pool of...

journal_title：FEMS microbiology letters

authors： Thomas MC,Thomas DK,Selinger LB,Inglis GD

更新日期：2011-07-01 00:00:00

abstract：:The 16S rDNA of 17 strains of Azospirillum, 14 assigned to one of the known species A. amazonense, A. brasilense, A. halopraeferens, A. irakense and A. lipoferum, and the other three of uncertain taxonomic position, was sequenced after polymerase chain reaction amplification and analysed in order to investigate the ph...

journal_title：FEMS microbiology letters

authors： Fani R,Bandi C,Bazzicalupo M,Ceccherini MT,Fancelli S,Gallori E,Gerace L,Grifoni A,Miclaus N,Damiani G

更新日期：1995-06-15 00:00:00

abstract：:We isolated a fungal strain HS-1 utilizing either ethylene glycol dibenzoate or ethyl benzoate as sole source of carbon and energy, and identified it as Aspergillus nomius, based on morphological and rDNA analyses. An enzyme hydrolyzing the esters was purified from the culture supernatant of the strain to an electroph...

journal_title：FEMS microbiology letters

authors： Uchida H,Kurakata Y,Sawamura H,Inamura N,Kotani T,Uwajima T

更新日期：2003-06-06 00:00:00

abstract：:Atypical psychrophilic Aeromonas salmonicida isolates were obtained from farmed and wild fish in Northeastern North America. These bacteria were isolated between 1992 and 2001 and carried tetracycline resistance (Tc(r)) plasmids of approximately 58 kb. The nine isolates had plasmids which could be divided into four gr...

journal_title：FEMS microbiology letters

authors： Casas C,Anderson EC,Ojo KK,Keith I,Whelan D,Rainnie D,Roberts MC

更新日期：2005-01-01 00:00:00

abstract：:Aspergillus niger represents a promising host for the expression of recombinant proteins, but only a few expression systems are available for this organism. In this study, the inducible catalase promoter (PcatR) from A. niger was characterized. For this, constructs were developed and checked for the expression of the ...

journal_title：FEMS microbiology letters

authors： Sharma R,Katoch M,Govindappa N,Srivastava PS,Sastry KN,Qazi GN

更新日期：2012-02-01 00:00:00

abstract：:The activities of some enzymes belonging to the Leloir pathway, phosphoglucomutase, UDP-glucose pyrophosphorylase, UDP-galactose 4-epimerase and galactose 1-P uridyl transferase, were studied in a wild ropy, a non-ropy and an overproducing mutant ropy strain of Streptococcus thermophilus. These activities were assayed...

journal_title：FEMS microbiology letters

authors： Escalante A,Villegas J,Wacher C,García-Garibay M,Farrés A

更新日期：2002-04-09 00:00:00

abstract：:A selection of influential FEMS publications to celebrate the 40th anniversary of FEMS. ...

journal_title：FEMS microbiology letters

authors： Prosser JI,Cole JA,Nielsen J,Bavoil PM,Häggblom MM

更新日期：2014-05-01 00:00:00

abstract：:Saccharomyces cerevisiae exponentially growing in basic or 0.7 M NaCl medium were isotopically labelled with 35S-methionine, followed by protein separation and quantification by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) combined with computerised image analysis. The electrophoretic separation resolv...

journal_title：FEMS microbiology letters

authors： Norbeck J,Blomberg A

更新日期：1996-03-15 00:00:00

abstract：:The study was made of the antibacterial effects of three terpene alcohols on Staphylococcus aureus, focusing on the leakage of K+ ions and toxicity over time. The leakage of K+ ions was monitored continuously with a K+-electrode. Our results suggested that the terpene alcohols, namely, farnesol, nerolidol and plaunoto...

journal_title：FEMS microbiology letters

authors： Inoue Y,Shiraishi A,Hada T,Hirose K,Hamashima H,Shimada J

更新日期：2004-08-15 00:00:00

abstract：:Primers designed to conserved regions of botulinum and tetanus clostridial toxins were used to amplify DNA fragments from non-proteolytic Clostridium botulinum type F (202F) DNA using polymerase chain reaction technology. The fragments were cloned and the complete nucleotide sequence of the gene encoding type F toxin ...

journal_title：FEMS microbiology letters

authors： East AK,Richardson PT,Allaway D,Collins MD,Roberts TA,Thompson DE

更新日期：1992-09-15 00:00:00

abstract：:A gene responsible for increased synthesis of hexaheme nitrite reductase (cytochrome c552) of Escherichia coli K-12 was cloned into pBR322 by the direct immunological screening method using antiserum against the purified enzyme. The cloned gene was mapped at 5 min on the chromosomal linkage map as the dni gene (relate...

journal_title：FEMS microbiology letters

authors： Kajie S,Ideta R,Yamato I,Anraku Y

更新日期：1991-10-01 00:00:00

abstract：:Pseudomonas pseudoalcaligenes strain YS1 isolated from oil contaminated soil was able to produce polyhydroxybutyrate blended with medium-chain-length polyhydroxyalkanoates (mcl PHA). PHA synthesis genes were cloned from this strain. A fadB (gene for fatty acid degradation) deleted mutant Escherichia coli KM32B (FADB::...

journal_title：FEMS microbiology letters

authors： Hang X,Lin Z,Chen J,Wang G,Hong K,Chen GQ

更新日期：2002-06-18 00:00:00

abstract：:Actinobacteria are known by their ability to produce several antimicrobial compounds of biotechnological interest. Thus, in this study, we isolated and identified by partial 16S RNA sequencing ∼100 actinobacteria isolates from guarana (Paullinia cupana) bulk soil. Besides, we isolated from the actinobacteria Streptomy...

journal_title：FEMS microbiology letters

authors： Vicente Dos Reis G,Abraham WR,Grigoletto DF,de Campos JB,Marcon J,da Silva JA,Quecine MC,de Azevedo JL,Ferreira AG,de Lira SP

更新日期：2019-07-01 00:00:00

abstract：:The sequence of 1383 nucleotides of the DNA encoding 16S rDNA was determined for strains of human intestinal spirochaetes, comprising an unnamed isolate and "Brachyspira aalborgi" NCTC 11492. A phylogenetic tree was inferred from aligned sequence comparisons between the intestinal spirochaetes, representatives of the ...

journal_title：FEMS microbiology letters

authors： Hookey JV,Barrett SP,Reed CS,Barber P

更新日期：1994-04-15 00:00:00

abstract：:A Gram-negative sporulating thermophilic anaerobe, designated AB11Ad, was isolated from the heated waters of the Great Artesian Basin of Australia. It grew on a variety of carbohydrates including glucose, starch, and dextran and produced a thermostable and thermoactive extracellular endo-dextranase. The enzyme was pro...

journal_title：FEMS microbiology letters

authors： Wynter C,Patel BK,Bain P,de Jersey J,Hamilton S,Inkerman PA

更新日期：1996-07-01 00:00:00

abstract：:This communication presents experimental evidence that intact fragile (osmotic sensitive) yeasts can be electrofused and give viable hybrids. The yield increases with one order of magnitude for electrofusion of intact fragile yeasts with protoplasts of non-fragile ones. The yield of viable hybrids, obtained by electro...

journal_title：FEMS microbiology letters

authors： Tsoneva I,Doinov P,Dimitrov DS

更新日期：1989-07-01 00:00:00

abstract：:Currently, interest in phage science is on the rise again as this subject remains largely unexplored, and its potential diversity mainly untapped. Here, we present phage vB_BsuP-Goe1, a new isolate that infects Bacillus subtilis The phage forms round plaques with a matt outline on agar plates containing a B. subtilis ...

journal_title：FEMS microbiology letters

authors： Willms IM,Hertel R

更新日期：2016-10-01 00:00:00

abstract：:We report the enhanced bactericidal activity of ofloxacin in drug-containing Eudragit E100(®) dispersions (EuCl-OFX) against Pseudomonas aeruginosa and the effect of the cationic polymer on bacterial membrane. Organisms treated with EuCl-OFX showed changes in cell morphology, altered outer membrane (OM) and cytoplasm...

journal_title：FEMS microbiology letters

authors： Romero VL,Pons P,Bocco JL,Manzo RH,Alovero FL

更新日期：2012-09-01 00:00:00

abstract：:Three different whole cell biosensor constructs were made by fusing the mercury inducible promoter, P(mer), and its regulatory gene, merR, from transposon Tn21 with reporter genes luxCDABE, lacZYA, or gfp. In Escherichia coli these biosensor constructs responded to low levels of mercury by producing light, beta-galact...

journal_title：FEMS microbiology letters

authors： Hansen LH,Sørensen SJ

更新日期：2000-12-01 00:00:00

abstract：:Recent studies have generated interest in the use of the homologous recombination system of bacteriophage lambda for genetic engineering. The system, called Red, consists primarily of three proteins: lambda exonuclease, which processively digests the 5'-ended strand of a dsDNA end; beta protein, which binds to ssDNA a...

journal_title：FEMS microbiology letters

authors： Poteete AR

更新日期：2001-07-10 00:00:00

abstract：:The majority of microorganisms in natural environments are difficult to cultivate, but their genes can be studied via metagenome libraries. To enhance the chances that these genes become expressed we here report the construction of a broad-host-range plasmid vector (pRS44) for fosmid and bacterial artificial chromosom...

journal_title：FEMS microbiology letters

authors： Aakvik T,Degnes KF,Dahlsrud R,Schmidt F,Dam R,Yu L,Völker U,Ellingsen TE,Valla S

更新日期：2009-06-01 00:00:00

abstract：:The objective of this work was to analyze the effects of thermosonication (TS) on induction of a viable but non-culturable (VBNC) state in Salmonella Typhimurium and to examine incubation factors affecting subsequent resuscitation of cells. A TS treatment of 380 W at 53°C for 30 min induced the VBNC state in S. Typhim...

journal_title：FEMS microbiology letters

authors： Liao H,Jiang L,Zhang R

更新日期：2018-02-01 00:00:00

abstract：:Lactobacillus johnsonii La1 (La1) is a probiotic strain capable of stimulating the immune system of the host and interfering with gastrointestinal pathogens. This study evaluates how the ingestion of different amounts of La1 influences the main bacterial populations of the fecal microbiota. Eight asymptomatic voluntee...

journal_title：FEMS microbiology letters

authors： Garrido D,Suau A,Pochart P,Cruchet S,Gotteland M

更新日期：2005-07-15 00:00:00

abstract：:The AbGst1 gene encoding a glutathione transferase from the necrotrophic pathogen Alternaria brassicicola was cloned from a benzyl isothiocyanate-treated conidial culture using differential display reverse transcription. The deduced amino-acid sequence of AbGst1p showed a significant degree of similarity to glutathion...

journal_title：FEMS microbiology letters

authors： Sellam A,Poupard P,Simoneau P

更新日期：2006-05-01 00:00:00

abstract：:A typing method for Clostridium difficile based on restriction fragment length polymorphisms (RFLP) is described. The technique utilizes commercially available Escherichia coli ribosomal ribonucleic acid (rRNA) as probe material. Probe labelling, hybridization and detection was performed using the Enhanced Chemilumine...

journal_title：FEMS microbiology letters

authors： Bowman RA,O'Neill GL,Riley TV

更新日期：1991-04-15 00:00:00

abstract：:To rapidly identify natural isolates of marine bioluminescent bacteria, we developed amplified ribosomal DNA restriction analysis (ARDRA) methods. ARDRA, which is based on the restriction patterns of 16S rRNA gene digested with five enzymes (EcoRI, DdeI, HhaI, HinfI, RsaI), clearly distinguished the 14 species of mari...

journal_title：FEMS microbiology letters

authors： Kita-Tsukamoto K,Wada M,Yao K,Kamiya A,Yoshizawa S,Uchiyama N,Kogure K

更新日期：2006-03-01 00:00:00

abstract：:Many bacteria are capable of accumulating intracellular granules of polyhydroxyalkanoates (PHA). In this work, we developed confocal microscopy analysis of bacterial cells to study changes in the diameters of cells as well as PHA granules during growth and PHA accumulation in the bacterium Cupriavidus necator H16 (for...

journal_title：FEMS microbiology letters

authors： Mravec F,Obruca S,Krzyzanek V,Sedlacek P,Hrubanova K,Samek O,Kucera D,Benesova P,Nebesarova J

更新日期：2016-05-01 00:00:00

abstract：:Surface antigen one (Sao) is a newly identified protein from the major zoonotic pathogen, Streptococcus suis. In search of functional proteins related to the pathogenesis of Chinese S. suis 2 (SS2), unexpectedly, a variant of Sao protein was obtained. To test its prevalence in S. suis, PCR assay was adopted to address...

journal_title：FEMS microbiology letters

authors： Feng Y,Zheng F,Pan X,Sun W,Wang C,Dong Y,Ju AP,Ge J,Liu D,Liu C,Yan J,Tang J,Gao GF

更新日期：2007-10-01 00:00:00

abstract：:The occurrence of highly variable penicillin-binding proteins (PBPs) in penicillin-resistant Streptococcus pneumoniae suggested that transfer of homologous genes from related species may be involved in resistance development. Antiserum and monoclonal antibodies raised against PBPs 1a and 2b from the susceptible S. pne...

journal_title：FEMS microbiology letters

authors： Chalkley L,Schuster C,Potgieter E,Hakenbeck R

更新日期：1991-12-15 00:00:00

abstract：:Blocking glycolytic breakdown of glucose by inactivation of phosphoglucose isomerase (Pgi) in Escherichia coli led to a greatly reduced maximum specific growth rate. Examination of the operational catabolic pathways and their flux ratios using [U-(13)C(6)]glucose-labeling experiments and metabolic flux ratio analysis ...

journal_title：FEMS microbiology letters

authors： Canonaco F,Hess TA,Heri S,Wang T,Szyperski T,Sauer U

更新日期：2001-11-13 00:00:00