Abstract:
:To rapidly identify natural isolates of marine bioluminescent bacteria, we developed amplified ribosomal DNA restriction analysis (ARDRA) methods. ARDRA, which is based on the restriction patterns of 16S rRNA gene digested with five enzymes (EcoRI, DdeI, HhaI, HinfI, RsaI), clearly distinguished the 14 species of marine bioluminescent bacteria currently known, which belong to the genera Vibrio, Photobacterium, and Shewanella. When we applied ARDRA to 129 natural isolates from two cruises in Sagami Bay, Japan, 127 were grouped into six ARDRA types with distinctive restriction patterns; these isolates represented the bioluminescent species, P. angustum, P. leiognathi, P. phosphoreum, S. woodyi, V. fischeri, and V. harveyi. The other two isolates showing unexpected ARDRA patterns turned out to have 16S rRNA gene sequences similar to P. leiognathi and P. phosphoreum. Nevertheless, ARDRA provides a simple and fairly robust means for rapid identification of the natural isolates of marine bioluminescent bacteria, and is therefore useful in studying their diversity.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Kita-Tsukamoto K,Wada M,Yao K,Kamiya A,Yoshizawa S,Uchiyama N,Kogure Kdoi
10.1111/j.1574-6968.2006.00129.xkeywords:
subject
Has Abstractpub_date
2006-03-01 00:00:00pages
298-303issue
2eissn
0378-1097issn
1574-6968pii
FML129journal_volume
256pub_type
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