Abstract:
:A Gram-negative sporulating thermophilic anaerobe, designated AB11Ad, was isolated from the heated waters of the Great Artesian Basin of Australia. It grew on a variety of carbohydrates including glucose, starch, and dextran and produced a thermostable and thermoactive extracellular endo-dextranase. The enzyme was produced more actively under pH controlled continuous culture conditions than under batch conditions. Ammonium sulfate precipitated crude dextranase exhibited a temperature optimum of 70 degrees C and a pH optimum between 5 and 6. The half life was approximately 6.5 h at 75 degrees C and 2 h at 80 degrees C at pH 5.0 and in the absence of added dextran. 16S rRNA sequence analysis indicated that isolate AB11Ad was a member of the genus Thermoanaerobacter.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Wynter C,Patel BK,Bain P,de Jersey J,Hamilton S,Inkerman PAdoi
10.1016/0378-1097(96)00193-0subject
Has Abstractpub_date
1996-07-01 00:00:00pages
271-6issue
2-3eissn
0378-1097issn
1574-6968pii
0378-1097(96)00193-0journal_volume
140pub_type
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