A novel thermostable dextranase from a Thermoanaerobacter species cultured from the geothermal waters of the Great Artesian Basin of Australia.

Abstract:

:A Gram-negative sporulating thermophilic anaerobe, designated AB11Ad, was isolated from the heated waters of the Great Artesian Basin of Australia. It grew on a variety of carbohydrates including glucose, starch, and dextran and produced a thermostable and thermoactive extracellular endo-dextranase. The enzyme was produced more actively under pH controlled continuous culture conditions than under batch conditions. Ammonium sulfate precipitated crude dextranase exhibited a temperature optimum of 70 degrees C and a pH optimum between 5 and 6. The half life was approximately 6.5 h at 75 degrees C and 2 h at 80 degrees C at pH 5.0 and in the absence of added dextran. 16S rRNA sequence analysis indicated that isolate AB11Ad was a member of the genus Thermoanaerobacter.

journal_name

FEMS Microbiol Lett

authors

Wynter C,Patel BK,Bain P,de Jersey J,Hamilton S,Inkerman PA

doi

10.1016/0378-1097(96)00193-0

subject

Has Abstract

pub_date

1996-07-01 00:00:00

pages

271-6

issue

2-3

eissn

0378-1097

issn

1574-6968

pii

0378-1097(96)00193-0

journal_volume

140

pub_type

杂志文章
  • Antifungal activity of a novel endochitinase gene (chit36) from Trichoderma harzianum Rifai TM.

    abstract::A novel 36-kDa endochitinase named chit36 has been isolated and characterized from Trichoderma harzianum Rifai TM. Partial amino acid sequences from the purified protein were used to clone the fungal cDNA, based on polymerase chain reaction with degenerate primers. The complete open reading frame encodes a 344-amino a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10710.x

    authors: Viterbo A,Haran S,Friesem D,Ramot O,Chet I

    更新日期:2001-06-25 00:00:00

  • ABC transporters in antibiotic-producing actinomycetes.

    abstract::Many antibiotic-producing actinomycetes possess at least one ABC (ATP-binding cassette) transporter which forms part of the antibiotic biosynthetic pathway and in most cases confers resistance to the drug in an heterologous host. Three types of antibiotic ABC transporters have been so far described in producer organis...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.1998.tb12792.x

    authors: Méndez C,Salas JA

    更新日期:1998-01-01 00:00:00

  • Use of intersimple sequence repeats markers to develop strain-specific SCAR markers for Lentinula edodes.

    abstract::Although Lentinula edodes is the second most important cultivated mushroom worldwide, most industrially cultivated strains have been identified only through traditional phenotypic analysis. Here, we report for the first time the use of sequence characterized amplified region (SCAR) markers for strain differentiation. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00145.x

    authors: Qin LH,Tan Q,Chen MJ,Pan YJ

    更新日期:2006-04-01 00:00:00

  • Molecular cloning and phylogenetic analysis of the small cytoplasmic RNA from Listeria monocytogenes.

    abstract::A molecular cloning strategy has been designed to isolate the gene that encodes the small cytoplasmic RNA (scRNA) component of bacterial signal recognition particles. Using this strategy a putative Listeria monocytogenes scRNA lambda gt11 recombinant clone was isolated. A previously described complementation assay dev...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13483.x

    authors: Barry T,Kelly M,Glynn B,Peden J

    更新日期:1999-04-01 00:00:00

  • Accelerated methanogenesis from aliphatic and aromatic hydrocarbons under iron- and sulfate-reducing conditions.

    abstract::The impact of four electron acceptors on hydrocarbon-induced methanogenesis was studied. Methanogenesis from residual hydrocarbons may enhance the exploitation of oil reservoirs and may improve bioremediation. The conditions to drive the rate-limiting first hydrocarbon-oxidizing steps for the conversion of hydrocarbon...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02165.x

    authors: Siegert M,Cichocka D,Herrmann S,Gründger F,Feisthauer S,Richnow HH,Springael D,Krüger M

    更新日期:2011-02-01 00:00:00

  • Production of lipopeptides in Bacillus sp. CS93 isolated from Pozol.

    abstract::Bacillus sp. strain CS93, which was previously isolated from Pozol, was previously shown to produce iturin A, bacilysin and chlorotetaine. To investigate the biosynthetic mechanism of chlorotetaine production, the bac genes were amplified from genomic DNA of Bacillus sp. CS93 by PCR and sequenced. The genes bacABCDE w...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01882.x

    authors: Moran S,Robertson K,Paradisi F,Rai DK,Murphy CD

    更新日期:2010-03-01 00:00:00

  • Candida albicans hyphal invasion: thigmotropism or chemotropism?

    abstract::Hyphae of the human pathogenic fungus Candida albicans exhibit thigmotropic behaviour in vitro, in common with phytopathogenic and saprotrophic fungi. An examination of the literature on C. albicans hyphal penetration of epithelial and endothelial membranes does not support the premise that hyphal thigmotropism plays ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.1999.tb13439.x

    authors: Davies JM,Stacey AJ,Gilligan CA

    更新日期:1999-02-15 00:00:00

  • Genomic analysis of Pseudomonas aeruginosa PA96, the host of carbapenem resistance plasmid pOZ176.

    abstract::Pseudomonas aeruginosa PA96 is a clinical isolate from Guangzhou, China, that is multiresistant to antibiotics. We previously described the 500-kb IncP-2 plasmid, pOZ176 that encodes many resistance genes including the IMP-9 carbapenemase. Whole-genome sequencing of PA96 enabled characterization of its genomic islands...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/1574-6968.12435

    authors: Déraspe M,Alexander DC,Xiong J,Ma JH,Low DE,Jamieson FB,Roy PH

    更新日期:2014-07-01 00:00:00

  • Accumulation of ppGpp in Streptococcus pyogenes and Streptococcus rattus following amino acid starvation.

    abstract::We have re-examined the stringent response of Streptococcus rattus and Streptococcus pyogenes, two organisms that had originally been reported not to accumulate ppGpp following amino acid deprivation. We conclude that ppGpp does accumulate when S. rattus and S. pyogenes are deprived of isoleucine by mupirocin addition...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb12836.x

    authors: Whitehead KE,Webber GM,England RR

    更新日期:1998-02-01 00:00:00

  • Lyme disease in transgenic mice expressing the Borrelia burgdorferi flagellin epitope implicated in human neuroborreliosis.

    abstract::Because of an association of human neuroborreliosis with the development of an antibody response against an antigen in neural tissue that cross-reacts with an epitope on the flagellin protein of Borrelia burgdorferi, C3H transgenic mice were created that expressed the flagellin epitope (amino acids 213-224) as a fusio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb10279.x

    authors: Fikrig E,Barthold SW

    更新日期:1997-03-15 00:00:00

  • Cloning, sequence analysis and heterologous expression of the Myrothecium gramineum orotidine-5'-monophosphate decarboxylase gene.

    abstract::A 2918 bp sequence coding for the orotidine-5'-monophosphate decarboxylase enzyme (OMPD) was isolated from the genome of Myrothecium gramineum. This sequence was analysed and, remarkably, it is the first OMPD gene of a Sordariomycete that has an intron. The gene codes for an enzyme of 282 amino acids. The nucleotide s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00373.x

    authors: De Maeseneire SL,De Groeve MR,Dauvrin T,De Mey M,Soetaert W,Vandamme EJ

    更新日期:2006-08-01 00:00:00

  • Existence and characterization of allelic variants of Sao, a newly identified surface protein from Streptococcus suis.

    abstract::Surface antigen one (Sao) is a newly identified protein from the major zoonotic pathogen, Streptococcus suis. In search of functional proteins related to the pathogenesis of Chinese S. suis 2 (SS2), unexpectedly, a variant of Sao protein was obtained. To test its prevalence in S. suis, PCR assay was adopted to address...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00859.x

    authors: Feng Y,Zheng F,Pan X,Sun W,Wang C,Dong Y,Ju AP,Ge J,Liu D,Liu C,Yan J,Tang J,Gao GF

    更新日期:2007-10-01 00:00:00

  • Esterase from the oil-degrading Acinetobacter lwoffii RAG-1: sequence analysis and over-expression in Escherichia coli.

    abstract::The est gene encoding an esterase from Acinetobacter lwoffii RAG-1 was cloned into E. coli under the control of the PL promoter of the phage lambda. The N-terminal sequence of the first 20 amino acids of the heterologous expressed esterase corresponded to that obtained from the nucleotide sequence. Antibodies prepared...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06462.x

    authors: Alon RN,Gutnick DL

    更新日期:1993-09-15 00:00:00

  • Occurrence of poly-D(-)-3-hydroxyalkanoates in the genus Bacillus.

    abstract::A range of Bacillus strains were examined for their ability to accumulate poly-D(-)-3-hydroxyalkanoates (poly-HAKs) which are naturally occurring materials that are optically active, biodegradable thermoplastics. The organisms could produce poly-D(-)-3-hydroxybutyrate (poly-HB) up to 50% of cell dry weight. The conten...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(91)90123-r

    authors: Chen GQ,König KH,Lafferty RM

    更新日期:1991-11-15 00:00:00

  • Isolation of a class IV chitin synthase gene from a zygomycete fungus, Rhizopus oligosporus.

    abstract::We found the presence of DNA sequence which shows sequence similarity to the class IV chitin synthase gene (CHS3) of Saccharomyces cerevisiae in the genome of 14 Rhizopus species which belong to zygomycetes. We cloned a gene (chs3), which might correspond to one of these homologous sequences, from Rhizopus oligosporus...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13291.x

    authors: Motoyama T,Horiuchi H,Ohta A,Yamaguchi I,Takagi M

    更新日期:1998-12-01 00:00:00

  • Functional analysis of Borrelia burgdorferi uvrA in DNA damage protection.

    abstract::Bacterial pathogens face constant challenges from DNA-damaging agents generated by host phagocytes. Although Borrelia burgdorferi appears to have much fewer DNA repair enzymes than pathogens with larger genomes, it does contain homologues of uvrA and uvrB (subunits A and B of excinuclease ABC). As a first step to expl...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02226.x

    authors: Sambir M,Ivanova LB,Bryksin AV,Godfrey HP,Cabello FC

    更新日期:2011-04-01 00:00:00

  • Extracellular polymeric substances responsible for bacterial adhesion onto solid surface.

    abstract::The influence of extracellular polymeric substances (EPS) on bacterial cell adhesion onto solid surfaces was investigated using 27 heterotrophic bacterial strains isolated from a wastewater treatment reactor. Cell adhesion onto glass beads was carried out by the packed-bed method and the results were discussed in term...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00399-9

    authors: Tsuneda S,Aikawa H,Hayashi H,Yuasa A,Hirata A

    更新日期:2003-06-27 00:00:00

  • A novel type-III staphylococcal cassette chromosome mec (SCCmec) variant among Indian isolates of methicillin-resistant Staphylococcus aureus.

    abstract::We identified a novel type-III staphylococcal cassette chromosome mec (SCCmec) element carried by eight methicillin-resistant Staphylococcus aureus (MRSA) strains from different wards and patients in an Indian hospital. Although the pulsed-field gel electrophoresis pattern and spa types of eight strains were identical...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01482.x

    authors: Arakere G,Nadig S,Ito T,Ma XX,Hiramatsu K

    更新日期:2009-03-01 00:00:00

  • Ca2+ stabilization of respiratory complex I from Escherichia coli.

    abstract::Stability of the membrane-bound and purified H+-translocating NADH:ubiquinone oxidoreductase, Complex I, was studied. The loss of the enzyme activity is strongly increased by alkaline pH and dilution of the sample. Complex I inactivation is prevented specifically by a low concentration of Ca2+ and/or an intracellular ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fny097

    authors: Belevich G,Belevich N,Knuuti J,Verkhovskaya M

    更新日期:2018-06-01 00:00:00

  • Isolation of Rhodococcus rhodochrous NCIMB13064 derivatives with new biodegradative abilities.

    abstract::Rhodococcus rhodochrous NCIMB13064 can dehalogenate and utilise a number of halogenated aliphatic compounds as sole carbon and energy source. Mutants of NCIMB13064 can be easily isolated with an enlarged range of 1-chloroalkane utilising ability. Dehalogenation of 1-chlorononane, 1-chlorodecane and short-chain 1-chlor...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08582.x

    authors: Kulakova AN,Reid KA,Larkin MJ,Allen CC,Kulakov LA

    更新日期:1996-12-01 00:00:00

  • Bacillus and Streptomyces were selected as broad-spectrum antagonists against soilborne pathogens from arid areas in Egypt.

    abstract::Plant protection via disease-suppressive bacteria in desert farming requires specific biological control agents (BCAs) adapted to the unique arid conditions. We performed an ecological study of below-ground communities in desert farm soil and untreated desert soil, and based on these findings, selected antagonists wer...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12089

    authors: Köberl M,Ramadan EM,Adam M,Cardinale M,Hallmann J,Heuer H,Smalla K,Berg G

    更新日期:2013-05-01 00:00:00

  • The identification of five genetic loci of Francisella novicida associated with intracellular growth.

    abstract::Five transposon mutants of Francisella novicida were isolated that are compromised in their ability to grow in mouse macrophages in vitro. Sequence analysis of the DNA flanking the transposon insertions identified the genes that were interrupted in these mutants. One of the inactivated loci corresponds to the Francise...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11369.x

    authors: Gray CG,Cowley SC,Cheung KK,Nano FE

    更新日期:2002-09-24 00:00:00

  • Occurrence of a lipase in spores of Alternaria brassicicola with a crucial role in the infection of cauliflower leaves.

    abstract::Alternaria brassicicola is a pathogen that penetrates directly through the host cuticle thanks to several serine esterases, according to our findings. Among these, an 80-kDa lipase (E.C 3.1.1. 3) was detected by SDS-PAGE and immunoblotting in the water washings of ungerminated spores. The purified lipase cross-reacted...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb08794.x

    authors: Berto P,Comménil P,Belingheri L,Dehorter B

    更新日期:1999-11-15 00:00:00

  • Morphological and intracellular alterations induced by cytotoxin VT2y produced by Escherichia coli isolated from chickens with swollen head syndrome.

    abstract::Recently, a novel verocytotoxin named VT2y was described which belongs to the STx family and is produced by Escherichia coli isolated from domestic poultry with swollen head syndrome (SHS). The VT2y toxin induced apoptosis in Vero, HeLa, CHO, CEF (primary chicken embryo fibroblast) and PCK (primary chicken kidney) cel...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10586.x

    authors: Salvadori MR,Yamada AT,Yano T

    更新日期:2001-04-01 00:00:00

  • Importance of fumarate and nitrate reduction regulatory protein for intestinal proliferation of Vibrio vulnificus.

    abstract::The sepsis caused by Vibrio vulnificus is characterized by an average incubation period of 26 h and a high mortality rate exceeding 50%. The fast growth and dissemination of V. vulnificus in vivo lead to poor clinical outcomes in patients. Therefore, elucidation of the proliferation mechanisms of this organism in vivo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw274

    authors: Kado T,Kashimoto T,Yamazaki K,Ueno S

    更新日期:2017-01-01 00:00:00

  • Correlation between the effects of fosfomycin and chloramphenicol on Escherichia coli.

    abstract::It was speculated that the increase of the UDP-GlcNAc pool observed with chloramphenicol can modulate the residual PEP:UDP-GlcNAc-enolpyruvate activity of fosfomycin-treated cells. This provided an explanation on how chloramphenicol can insure the formation of enough UDP-MurNAc-pentapeptide to sustain peptidoglycan sy...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90270-z

    authors: Mengin-Lecreulx D,van Heijenoort J

    更新日期:1990-01-01 00:00:00

  • Programmed cell death and the pathogenesis of tissue injury induced by type A Francisella tularensis.

    abstract::Francisella tularensis is a highly virulent bacterial species that causes various forms of tularemia in humans. The urgency in understanding the pathogenesis of these diseases has stimulated unprecedented interest in this bacterial species over the past few years. Recent findings underscore a number of important disti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.2009.01791.x

    authors: Parmely MJ,Fischer JL,Pinson DM

    更新日期:2009-11-01 00:00:00

  • Genetic evidence for a novel thymidylate synthase in the halophilic archaeon Halobacterium salinarum and in Campylobacter jejuni.

    abstract::A search of the complete genome sequence of the halophilic archaeon Halobacterium salinarum failed to identify a gene homologous to the thymidylate synthase (thyA) gene present in the closely related Haloferax volcanii. To understand the source of thymidine synthesis in Hbt. salinarum, a genomic library of Hbt. salina...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11422.x

    authors: Giladi M,Bitan-Banin G,Mevarech M,Ortenberg R

    更新日期:2002-10-29 00:00:00

  • Role of flagellum and chemotactic motility of Vibrio anguillarum for phagocytosis by and intracellular survival in fish macrophages.

    abstract::The role of the flagellum and chemotactic motility of Vibrio anguillarum for phagocytosis by and intracellular survival in fish macrophages was determined using a wild-type strain, a mutant without the flagellum, a mutant with a truncated flagellum and a non-chemotactic mutant. For all strains, the numbers of intracel...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10833.x

    authors: Larsen MH,Boesen HT

    更新日期:2001-09-25 00:00:00

  • The phylogeny of Aerococcus and Pediococcus as determined by 16S rRNA sequence analysis: description of Tetragenococcus gen. nov.

    abstract::The phylogenetic interrelationships of the genera Pediococcus and Aerococcus were investigated using reverse transcriptase sequencing of 16S rRNA. The genus Pediococcus was found to be phylogenetically heterogeneous. The four species P. acidilactici, P. damnosus, P. parvulus and P. pentosaceus formed a phylogeneticall...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/s0378-1097(05)80004-7

    authors: Collins MD,Williams AM,Wallbanks S

    更新日期:1990-08-01 00:00:00