Metabolic flux response to phosphoglucose isomerase knock-out in Escherichia coli and impact of overexpression of the soluble transhydrogenase UdhA.

abstract：:DNA polymerase from the archaeon Pyrococcus abyssi strain Orsay was expressed in Escherichia coli. The recombinant DNA polymerase (Pab) was purified to homogeneity by heat treatment followed by 5 steps of chromatography and characterized for PCR applications. Buffer optimization experiments indicated that Pab PCR perf...

journal_title：FEMS microbiology letters

authors： Dietrich J,Schmitt P,Zieger M,Preve B,Rolland JL,Chaabihi H,Gueguen Y

更新日期：2002-11-19 00:00:00

abstract：:Antibiotic susceptibility, plasmid profiles and random amplification of polymorphic DNA (RAPD) were used to study strains of Vibrio vulnificus isolated from cockles (Anadara granosa). Thirty-six isolates were analyzed. The prevalent biotypes were 1 (72.2% of the isolates) and 2 (27.8%). Among these, 21 strains of biot...

journal_title：FEMS microbiology letters

authors： Radu S,Elhadi N,Hassan Z,Rusul G,Lihan S,Fifadara N,Yuherman,Purwati E

更新日期：1998-08-01 00:00:00

abstract：:We have determined the genetic basis of minocycline resistance in a strain of Streptococcus infantis isolated from a healthy human oral cavity. We demonstrate that tet(S), identical to tet(S) found on the enterococcal conjugative transposon Tn6000, is responsible for the observed resistance. The gene is located on a s...

journal_title：FEMS microbiology letters

authors： Ciric L,Brouwer MS,Mullany P,Roberts AP

更新日期：2014-04-01 00:00:00

abstract：:The symbiotic plasmid (pSym) DNA present in bacteroids of strain RCR1001 of Rhizobium leguminosarum biovar viceae has been compared qualitatively and quantitatively with that present in free living bacteria by hybridization experiments with appropriate probes. A decrease in the relative amount of pSym DNA was observed...

journal_title：FEMS microbiology letters

authors： Vierny C,Iaccarino M

更新日期：1989-07-01 00:00:00

abstract：:Penicillium expansum is the causal agent of blue mold rot, a postharvest decay of stored fruits. This fungus also produces the mycotoxins patulin and citrinin. Control of P. expansum still relies mainly on the use of fungicides such as thiabendazole. Since its introduction, resistant strains have been reported. The ai...

journal_title：FEMS microbiology letters

authors： Cabañas R,Castellá G,Abarca ML,Bragulat MR,Cabañes FJ

更新日期：2009-08-01 00:00:00

abstract：:Proteins which are secreted or associated with the cell envelope of Mycobacterium tuberculosis may contain protective T-cell epitopes. Prior to this study, a recombinant clone bank of enzymatically active M. tuberculosis-alkaline phosphatase fusions, were screened for immunogenicity in a murine T-cell model. Five of t...

journal_title：FEMS microbiology letters

authors： Moran AJ,Doran JL,Wu J,Treit JD,Ekpo P,Kerr VJ,Roberts AD,Orme IM,Galant S,Ress SR,Nano FE

更新日期：1999-08-01 00:00:00

abstract：:In Streptomyces cyanogenus S136 gene cluster for biosynthesis of polyglycosylated angucycline landomycin A (LaA), a divergently oriented gene pair for a TetR-family regulator (lanK) and an efflux protein (lanJ) is located, whose functions remained obscure. Overexpression and disruption studies showed that lanK and lan...

journal_title：FEMS microbiology letters

authors： Ostash I,Ostash B,Luzhetskyy A,Bechthold A,Walker S,Fedorenko V

更新日期：2008-08-01 00:00:00

abstract：:The chrysene-degrading bacterium Pseudoxanthomonas sp. PNK-04 was isolated from a coal sample. Three novel metabolites, hydroxyphenanthroic acid, 1-hydroxy-2-naphthoic acid and salicylic acid, were identified by TLC, HPLC and MS. Key enzyme activities, namely 1-hydroxy-2-naphthoate hydroxylase, 1,2-dihydroxynaphthalen...

journal_title：FEMS microbiology letters

authors： Nayak AS,Sanjeev Kumar S,Santosh Kumar M,Anjaneya O,Karegoudar TB

更新日期：2011-07-01 00:00:00

abstract：:Methanogenic archaea conserve energy for growth by reducing some one- and two-carbon compounds to methane and concomitantly generating an ion motive force. Growth of Methanosarcina acetivorans on carbon monoxide (CO) is peculiar as it involves formation of, besides methane, formate, acetate and methylated thiols. It h...

journal_title：FEMS microbiology letters

authors： Oelgeschläger E,Rother M

更新日期：2009-03-01 00:00:00

abstract：:An internal segment of the penicillin-binding protein gene, pbpA, of Streptomyces griseus was amplified from genomic DNA using the polymerase chain reaction and used as a hybridization probe to isolate the complete gene from a cosmid library. pbpA encodes a 485 amino acid sequence that conserves three motifs of PBPs, ...

journal_title：FEMS microbiology letters

authors： Jiang H,Kendrick KE

更新日期：2000-12-01 00:00:00

abstract：:Phylogenetic relationships between Tuber pseudoexcavatum and other Tuber species were investigated by studying the sequences of four genes: 5.8S-ITS2, beta-tubulin, protein kinase C and elongation factor 1alpha. The four phylogenetic trees allowed to differentiate the black truffle clade, composed of two subclades, on...

journal_title：FEMS microbiology letters

authors： Wang Y,Tan ZM,Zhang DC,Murat C,Jeandroz S,Le Tacon F

更新日期：2006-06-01 00:00:00

abstract：:Streptococcus suis is a worldwide cause of various swine infections and is also an important agent of zoonosis. Strains of S. suis are classified according to their serotype, and currently, 35 serotypes are recognized. The aim of this study was to characterize nontypeable isolates of S. suis with regard to their cell ...

journal_title：FEMS microbiology letters

authors： Bonifait L,Gottschalk M,Grenier D

更新日期：2010-10-01 00:00:00

abstract：:Staphylococcus aureus is responsible for a broad variety of chronic infections. Most S. aureus clinical isolates show the capacity to adhere to abiotic surfaces and to develop biofilms. Because S. aureus growing in a biofilm is highly refractory to treatment, inhibition of biofilm formation represents a major therapeu...

journal_title：FEMS microbiology letters

authors： Stenz L,François P,Fischer A,Huyghe A,Tangomo M,Hernandez D,Cassat J,Linder P,Schrenzel J

更新日期：2008-10-01 00:00:00

abstract：:The CotSA protein, encoded by cotSA (ytxN) of Bacillus subtilis, was detected from the cells at 5 h after the onset of sporulation (T5) and in the spore coat of wild-type cells, but not in cotE, cotS, gerE, or cotSA mutant spores. CotSA was also detected in the sporangium at T5 to T7 but not in the sporangium at T18 o...

journal_title：FEMS microbiology letters

authors： Takamatsu H,Kodama T,Watabe K

更新日期：1999-05-01 00:00:00

abstract：:The study was made of the antibacterial effects of three terpene alcohols on Staphylococcus aureus, focusing on the leakage of K+ ions and toxicity over time. The leakage of K+ ions was monitored continuously with a K+-electrode. Our results suggested that the terpene alcohols, namely, farnesol, nerolidol and plaunoto...

journal_title：FEMS microbiology letters

authors： Inoue Y,Shiraishi A,Hada T,Hirose K,Hamashima H,Shimada J

更新日期：2004-08-15 00:00:00

abstract：:The activities of some enzymes belonging to the Leloir pathway, phosphoglucomutase, UDP-glucose pyrophosphorylase, UDP-galactose 4-epimerase and galactose 1-P uridyl transferase, were studied in a wild ropy, a non-ropy and an overproducing mutant ropy strain of Streptococcus thermophilus. These activities were assayed...

journal_title：FEMS microbiology letters

authors： Escalante A,Villegas J,Wacher C,García-Garibay M,Farrés A

更新日期：2002-04-09 00:00:00

abstract：:The galE gene of Pasteurella multocida has been isolated by complementing galE-defective mutants of Salmonella typhimurium with a plasmid library of this organism. The complete nucleotide sequence of the P. multocida galE gene consists of 1017 nucleotides, encoding a predicted polypeptide of 339 amino acids. The deduc...

journal_title：FEMS microbiology letters

authors： Fernández de Henestrosa AR,Badiola I,Saco M,Perez de Rozas AM,Campoy S,Barbé J

更新日期：1997-09-15 00:00:00

abstract：:A CO-reactive hemoprotein was purified from the mitochondrial membrane fraction of Tetrahymena pyriformis. It showed absorption peaks at 615 and 455 nm in the reduced form and an alpha peak at 565 nm in the pyridine ferrohemochrome spectrum. Although the spectral properties were apparently similar to those of 'cytochr...

journal_title：FEMS microbiology letters

authors： Inokuchi A,Fukumori Y

更新日期：1993-08-15 00:00:00

abstract：:In this study, 29 strains of Kluyveromyces marxianus with peculiar genetic and phenotypic traits previously isolated from a fermented goat milk of Yaghnob valley were investigated for chromosome length polymorphism (CLP) by PFGE, adhesion properties and carbon usage by Biolog analysis. Obtained data showed that strain...

journal_title：FEMS microbiology letters

authors： Perpetuini G,Tittarelli F,Mattarelli P,Modesto M,Cilli E,Suzzi G,Tofalo R

更新日期：2018-03-01 00:00:00

abstract：:The Bacteroides fragilis enterotoxin is an extracellular zinc metalloprotease that has been implicated in diarrheal disease of humans and animals. This toxin causes fluid accumulation in intestinal loops and is cytotoxic for HT-29 cells, an intestinal carcinoma cell line. Here we report the cloning and sequencing of t...

journal_title：FEMS microbiology letters

authors： Kling JJ,Wright RL,Moncrief JS,Wilkins TD

更新日期：1997-01-15 00:00:00

abstract：:The expression of the rbp genes, which encode RNA-binding proteins with a single RNA-recognition motif and a glycine-rich sequence, is known to increase at low temperature in cyanobacteria. We previously showed that their regulation involved both transcription and mRNA stability. In the present study, various reporter...

journal_title：FEMS microbiology letters

authors： Ehira S,Ohmori M,Sato N

更新日期：2005-10-01 00:00:00

abstract：:Endophytic fungi from Paeonia lactiflora Pallas, which is mainly distributed in China, were characterized and screened to identify those capable of producing paeoniflorin. A total of 101 isolates obtained from the roots, stems and leaves of P. lactiflora were grouped into 16 fungal taxa based on morphological traits a...

journal_title：FEMS microbiology letters

authors： Cheng X,Wei Z,Pu S,Xiang M,Yan A,Zhang Y,Wang X

更新日期：2018-12-01 00:00:00

abstract：:Intact nuclei were isolated from the protoplasts of the filamentous fungus Aspergillus nidulans. The large amounts of protoplasts required for such nuclear preparations were produced from young mycelia grown in liquid culture. For final purification of the crude nuclear fraction, a Nycodenz density-gradient centrifuga...

journal_title：FEMS microbiology letters

authors： Vágvölgyi C,Ferenczy L

更新日期：1991-08-15 00:00:00

abstract：:The enterotoxigenic Escherichia coli (ETEC) strain Ec2173, causing post weaning diarrhoea in swine, harbours six plasmids ranging from 13 to 200 kb in size. The heat stable toxin genes sta, stb and a tetracycline resistance gene were located on a self conjugative 120-kb plasmid, called pTC. In the cloned ColE1 type or...

journal_title：FEMS microbiology letters

authors： Olasz F,Fekete PZ,Blum-Oehler G,Boldogkoi Z,Nagy B

更新日期：2005-03-15 00:00:00

abstract：:We report a rapid and reliable two-step multiplex polymerase chain reaction (PCR) assay to identify the 10 Bacteroides fragilis group species - Bacteroides caccae, B. distasonis, B. eggerthii, B. fragilis, B. merdae, B. ovatus, B. stercoris, B. thetaiotaomicron, B. uniformis and B. vulgatus. These 10 species were firs...

journal_title：FEMS microbiology letters

authors： Liu C,Song Y,McTeague M,Vu AW,Wexler H,Finegold SM

更新日期：2003-05-16 00:00:00

abstract：:We isolated and characterized nodA genes from photosynthetic and non-photosynthetic rhizobia nodulating the legume genus Aeschynomene, and found that the nodA sequence from photosynthetic stem-nodulating bacteria was phylogenetically distant from the other already described nodA genes. Characterization of the photosyn...

journal_title：FEMS microbiology letters

authors： Chaintreuil C,Boivin C,Dreyfus B,Giraud E

更新日期：2001-01-01 00:00:00

abstract：:To explore the discriminatory power of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for detecting subtle differences in isogenic isolates, we tested isogenic strains of Staphylococcus aureus differing in their expression of resistance to methicillin or teicoplanin. More i...

journal_title：FEMS microbiology letters

authors： Majcherczyk PA,McKenna T,Moreillon P,Vaudaux P

更新日期：2006-02-01 00:00:00

abstract：:Mathematical modeling is an important tool in biological research, allowing for the synthesis of results from many studies into an understanding of a system. Despite this, the need for extensive subject matter knowledge and complex mathematics often leaves modeling as an esoteric subspecialty. A 2-fold approach can be...

journal_title：FEMS microbiology letters

authors： Lofgren ET

更新日期：2016-10-01 00:00:00

abstract：:The growing demand to replace fossil fuels with renewable alternatives has generated an urgent and imminent global need to find new non-fossil sources. Sweet sorghum is widely recognized as a highly promising biomass energy crop with the particular potential to complement sugarcane for ethanol production. Our aim in t...

journal_title：FEMS microbiology letters

authors： Durán MA,Dantur K,Ruiz RM,Romero ER,Zossi S,Gusils C

更新日期：2018-07-01 00:00:00

abstract：:The variable stress-sensitivity of individual cells within pure cultures is widely noted but generally unexplained. Here, factors determining the heterogeneous susceptibility to copper toxicity in Saccharomyces cerevisiae were examined with a rapid non-perturbing approach based on flow cytometry. By determination of t...

journal_title：FEMS microbiology letters

authors： Howlett NG,Avery SV

更新日期：1999-07-15 00:00:00