A catabolic pathway for the degradation of chrysene by Pseudoxanthomonas sp. PNK-04.

Abstract:

:The chrysene-degrading bacterium Pseudoxanthomonas sp. PNK-04 was isolated from a coal sample. Three novel metabolites, hydroxyphenanthroic acid, 1-hydroxy-2-naphthoic acid and salicylic acid, were identified by TLC, HPLC and MS. Key enzyme activities, namely 1-hydroxy-2-naphthoate hydroxylase, 1,2-dihydroxynaphthalene dioxygenase, salicylaldehyde dehydrogenase and catechol-1,2-dioxygenase, were noted in the cell-free extract. These results suggest that chrysene is catabolized via hydroxyphenanthroic acid, 1-hydroxy-2-naphthoic acid, salicylic acid and catechol. The terminal aromatic metabolite, catechol, is then catabolized by catechol-1,2-dioxygenase to cis,cis-muconic acid, ultimately forming TCA cycle intermediates. Based on these studies, the proposed catabolic pathway for chrysene degradation by strain PNK-04 is chrysene → hydroxyphenanthroic acid → 1-hydroxy-2-naphthoic acid → 1,2-dihydroxynaphthalene → salicylic acid → catechol →cis,cis-muconic acid.

journal_name

FEMS Microbiol Lett

authors

Nayak AS,Sanjeev Kumar S,Santosh Kumar M,Anjaneya O,Karegoudar TB

doi

10.1111/j.1574-6968.2011.02301.x

subject

Has Abstract

pub_date

2011-07-01 00:00:00

pages

128-34

issue

2

eissn

0378-1097

issn

1574-6968

journal_volume

320

pub_type

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