The Cyclic AMP-binding protein CbpB in Brucella melitensis and its role in cell envelope integrity, resistance to detergent and virulence.

abstract：:A cryptic plasmid from Arthrobacter rhombi PRH1, designated as pPRH, was sequenced and characterized. It was 5000 bp in length with a G+C content of 66 mol%. The plasmid pPRH was predicted to encode six putative open reading frames (ORFs), in which ORF2 and ORF3 formed the minimal replicon of plasmid pPRH and shared 5...

journal_title：FEMS microbiology letters

authors： Stanislauskiene R,Gasparaviciute R,Vaitekunas J,Meskiene R,Rutkiene R,Casaite V,Meskys R

更新日期：2012-02-01 00:00:00

abstract：:The Mycobacterium marinum G13 promoter is a sigma 70-like promoter that is more active by green fluorescent protein (gfp) differential fluorescence induction (DFI) assays when M. marium resides in an intracellular compartment as compared with growth in media. In assays using DFI, we found that the mycobacterial G13 pr...

journal_title：FEMS microbiology letters

authors： Gall K,Barker LP

更新日期：2006-02-01 00:00:00

abstract：:A Na(+)/H(+) antiporter gene was isolated from alkaliphilic Bacillus sp. G1. The full-length sequence of the Na(+)/H(+) antiporter gene was obtained using a genome walking method, and designated as g1-nhaC. An ORF preceded by a promoter-like sequence and a Shine-Dalgarno sequence, and followed by a terminator-like seq...

journal_title：FEMS microbiology letters

authors： Liew CW,Illias RM,Mahadi NM,Najimudin N

更新日期：2007-11-01 00:00:00

abstract：:Streptococcus pneumoniae strain JNR.7/87 is a highly virulent, type 4 encapsulated Gram-positive bacterium whose transformability has not been tested previously, and whose genome is currently being sequenced. The strain was transformed at very low efficiency by addition of exogenous competence-stimulating peptide: How...

journal_title：FEMS microbiology letters

authors： Bricker AL,Camilli A

更新日期：1999-03-15 00:00:00

abstract：:Stability of the membrane-bound and purified H+-translocating NADH:ubiquinone oxidoreductase, Complex I, was studied. The loss of the enzyme activity is strongly increased by alkaline pH and dilution of the sample. Complex I inactivation is prevented specifically by a low concentration of Ca2+ and/or an intracellular ...

journal_title：FEMS microbiology letters

authors： Belevich G,Belevich N,Knuuti J,Verkhovskaya M

更新日期：2018-06-01 00:00:00

abstract：:Rhizobium sp. SIN-1, isolated in India from root nodules on the tropical legume Sesbania aculeata, also induces nitrogen-fixing nodules on roots of S. macrocarpa, S. speciosa, S. procumbens, S. punicea, S. rostrata, and Vigna unguiculata. Unlike Azorhizobium caulinodans, SIN-1 does not induce stem nodules on S. rostra...

journal_title：FEMS microbiology letters

authors： Rana D,Krishnan HB

更新日期：1995-12-01 00:00:00

abstract：:The distribution of the antiseptic-resistance genes qacE and qacE delta 1 was studied in a large number of Gram-negative bacteria by a method that included the polymerase chain reaction (PCR). A total of 117 strains of Gram-negative bacteria, isolated from clinical or environmental sources, was used in this analysis. ...

journal_title：FEMS microbiology letters

authors： Kazama H,Hamashima H,Sasatsu M,Arai T

更新日期：1998-02-15 00:00:00

abstract：:Mycoplasma salivarium cells bound the Fc fragment of human immunoglobulin G. The activity was remarkably enhanced by Mn2+, but not by Mg2+ and Ca2+; significantly inhibited by D-mannose; and reduced by pronase treatment of the cells. About 90% of the cells treated with pronase were not disrupted. Sodium dodecyl sulfat...

journal_title：FEMS microbiology letters

authors： Shibata K,Sawa Y,Inoue S,Noda M,Watanabe T

更新日期：1994-11-01 00:00:00

abstract：:The presence and distribution of isoprenoid glycerol dialkyl glycerol tetraethers (GDGTs), lipids that constitute the membranes of Archaea, have been investigated in a 50-cm long core from a Swedish peat bog. In the acrotelm, the periodically water saturated and thus oxic upper layer of the peat bog, only minor amount...

journal_title：FEMS microbiology letters

authors： Weijers JW,Schouten S,van der Linden M,van Geel B,Damsté JS

更新日期：2004-10-01 00:00:00

abstract：:The response regulator proteins of two-component systems mediate many adaptations of bacteria to their ever-changing environment. Most response regulators are transcription factors that alter the level of transcription of specific sets of genes. Activation of response regulators requires their phosphorylation on a con...

journal_title：FEMS microbiology letters

authors： Ladds JC,Muchová K,Blaskovic D,Lewis RJ,Brannigan JA,Wilkinson AJ,Barák I

更新日期：2003-06-27 00:00:00

abstract：:We report the construction of two broad host range promoter-probe plasmid vectors for rapid analysis of promoters in Gram-negative bacteria. The new vectors, pME4507 and pME4510, carry carbenicillin and gentamycin resistance genes, respectively, and are small sized (4 kb) with a flexible multiple cloning site to facil...

journal_title：FEMS microbiology letters

authors： Rist M,Kertesz MA

更新日期：1998-12-01 00:00:00

abstract：:Saccharomyces cerevisiae exponentially growing in basic or 0.7 M NaCl medium were isotopically labelled with 35S-methionine, followed by protein separation and quantification by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) combined with computerised image analysis. The electrophoretic separation resolv...

journal_title：FEMS microbiology letters

authors： Norbeck J,Blomberg A

更新日期：1996-03-15 00:00:00

abstract：:Brief exposure of Pseudomonas aeruginosa to a temperature of 10 degrees C or lower caused a significant leakage of the periplasmic beta-lactamase into the medium. The extent of leakage increased as the incubation temperature was lowered to 4 degrees C and reached a maximum at 0 degrees C. Cells grown in the presence o...

journal_title：FEMS microbiology letters

authors： Lei Y,Satake S,Ishii J,Nakae T

更新日期：1991-05-15 00:00:00

abstract：:Pseudomonas aeruginosa biofilm formation was increased by addition of sucrose to Luria-Bertani medium, whereas addition of NaCl to a final similar osmolarity and use of maltose instead of sucrose, were ineffective. In a previous study, we showed that the extracytoplasmic sigma factor SigX is activated in the presence ...

journal_title：FEMS microbiology letters

authors： Bouffartigues E,Duchesne R,Bazire A,Simon M,Maillot O,Dufour A,Feuilloley M,Orange N,Chevalier S

更新日期：2014-07-01 00:00:00

abstract：:Methods for in vivo monitoring of redox changes in different cellular compartments have been developed in recent years, and are mostly based on redox-sensitive variants of the green fluorescent protein (GFP). However, due to the thermodynamic stability of the introduced reactive disulfide bond, these sensors are limit...

journal_title：FEMS microbiology letters

authors： Delic M,Mattanovich D,Gasser B

更新日期：2010-05-01 00:00:00

abstract：:In spite of being dispensable, O-antigens are believed to facilitate various cellular processes and alter antibiotic sensitivities. Escherichia coli K-12 (CS109) strains are lacking in O-antigens and are reported to be sensitive to antibiotics. To our surprise, E. coli 2443 (expressing O8-antigen) manifested two- to f...

journal_title：FEMS microbiology letters

authors： Sarkar SK,Ghosh AS

更新日期：2008-05-01 00:00:00

abstract：:Bacillus subtilis strains BS49 and BS34A, both derived from a common ancestor, carry one or more copies of Tn916, an extremely common mobile genetic element capable of transfer to and from a broad range of microorganisms. Here, we report the complete genome sequence of BS49 and the draft genome sequence of BS34A, whic...

journal_title：FEMS microbiology letters

authors： Browne HP,Anvar SY,Frank J,Lawley TD,Roberts AP,Smits WK

更新日期：2015-01-01 00:00:00

abstract：:Spores of Bacillus species have novel properties, which allow them to lie dormant for years and then germinate under favourable conditions. In the current work, the role of a key metabolic integrator, coenzyme A (CoA), in redox regulation of growing cells and during spore formation in Bacillus megaterium and Bacillus ...

journal_title：FEMS microbiology letters

authors： Zhyvoloup A,Yu BYK,Baković J,Davis-Lunn M,Tossounian MA,Thomas N,Tsuchiya Y,Peak-Chew SY,Wigneshweraraj S,Filonenko V,Skehel M,Setlow P,Gout I

更新日期：2020-12-22 00:00:00

abstract：:A previous study [DeVault et al. (1991) Mol. Microbiol. 5, 2503-2509] suggested that growth of Pseudomonas aeruginosa in glucose-containing medium represses algD gene transcription. In this study, growth of P. aeruginosa in rich medium containing glucose or gluconate increased alginate production and algD transcriptio...

journal_title：FEMS microbiology letters

authors： Ma JF,Phibbs PV,Hassett DJ

更新日期：1997-03-15 00:00:00

abstract：:Two actinomycete strains, CHR3 and CHR28, were isolated from metal-contaminated sediments from Baltimore Inner Harbor. The isolates were classified as Streptomyces spp. Agar diffusion assays showed both isolates to be resistant to mercuric chloride and phenylmercuric acetate. Hybridization experiments indicated that g...

journal_title：FEMS microbiology letters

authors： Ravel J,Amoroso MJ,Colwell RR,Hill RT

更新日期：1998-05-01 00:00:00

abstract：:A search of the complete genome sequence of the halophilic archaeon Halobacterium salinarum failed to identify a gene homologous to the thymidylate synthase (thyA) gene present in the closely related Haloferax volcanii. To understand the source of thymidine synthesis in Hbt. salinarum, a genomic library of Hbt. salina...

journal_title：FEMS microbiology letters

authors： Giladi M,Bitan-Banin G,Mevarech M,Ortenberg R

更新日期：2002-10-29 00:00:00

abstract：:Himalaya hosts a unique environment for microbial ecology. The present study aimed to explore the bioplastic producing bacterial communities along altitude gradient of Pangi-Chamba trans-Himalayan region. A total of 411 bacteria were isolated and 70 characterized at the molecular level for potential polyhydroxyalkanoa...

journal_title：FEMS microbiology letters

authors： Kumar V,Thakur V,Ambika,Kumar S,Singh D

更新日期：2018-07-01 00:00:00

abstract：:L-allo-Threonine aldolase (L-allo-threonine acetaldehyde-lyase), which exhibited specificity for L-allo-threonine but not for L-threonine, was purified from a cell-free extract of Aeromonas jandaei DK-39. The purified enzyme catalyzed the aldol cleavage reaction of L-allo-threonine (K(m) = 1.45 mM, Vmax = 45.2 mumol m...

journal_title：FEMS microbiology letters

authors： Kataoka M,Wada M,Nishi K,Yamada H,Shimizu S

更新日期：1997-06-15 00:00:00

abstract：:We found that the presence of plasmids expressing tetracycline resistance or chloramphenicol resistance genes, but not those expressing ampicillin resistance or kanamycin resistance genes, in Escherichia coli led to the retardation of the process of removal of the heat-aggregated proteins (i.e. the S fraction) from th...

journal_title：FEMS microbiology letters

authors： Kedzierska S,Staniszewska M,Potrykus J,Wegrzyn G

更新日期：1999-07-15 00:00:00

abstract：:Fusarium graminearum was grown on four lignocellulosic substrates (corn cobs, wheat bran, hop cell walls, and birchwood) and glucose as the sole carbon source. Proteomic studies performed on the resulting enzymatic cocktails highlighted a great diversity in the number and type of proteins secreted. The cell wall-degra...

journal_title：FEMS microbiology letters

authors： Debeire P,Delalande F,Habrylo O,Jeltsch JM,Van Dorsselaer A,Phalip V

更新日期：2014-06-01 00:00:00

abstract：:The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma 70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from t...

journal_title：FEMS microbiology letters

authors： Brans A,Loriaux A,Thamm I,Joris B,Dusart J

更新日期：1997-04-15 00:00:00

abstract：:Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) represents a simple reliable approach for rapid bacterial identification based on specific peptide/protein fingerprints. However, cell-wall characteristics of mycobacterial species, and their well known stability, complicate MA...

journal_title：FEMS microbiology letters

authors： Balážová T,Makovcová J,Šedo O,Slaný M,Faldyna M,Zdráhal Z

更新日期：2014-04-01 00:00:00

abstract：:The study of exopolysaccharide production by heterofermentative sourdough lactic acid bacteria has shown that Weissella strains isolated from sourdoughs produce linear dextrans containing α-(1→6) glucose residues with few α-(1→3) linkages from sucrose. In this study, several dextran-producing strains, Weissella cibari...

journal_title：FEMS microbiology letters

authors： Bounaix MS,Robert H,Gabriel V,Morel S,Remaud-Siméon M,Gabriel B,Fontagné-Faucher C

更新日期：2010-10-01 00:00:00

abstract：:Leflunomide (LFM) is a novel anti-inflammatory and immunosuppressive drug, and inhibits the growth of cytokine-stimulated lymphoid cells in vitro. The effect of LFM on haploid and diploid cells of Saccharomyces cerevisiae was investigated to elucidate the molecular mechanism of action of the drug. Using a halo assay, ...

journal_title：FEMS microbiology letters

authors： Fujimura H

更新日期：2000-10-01 00:00:00

abstract：:Using natural rubber latex as the sole source of carbon and energy 50 rubber-degrading bacteria were isolated. Out of those 50 isolates, 33 were identified as Streptomyces species and 8 as Micromonospora species. Screening of 1220 bacteria obtained from different culture collections revealed 46 additional rubber-degra...

journal_title：FEMS microbiology letters

authors： Jendrossek D,Tomasi G,Kroppenstedt RM

更新日期：1997-05-15 00:00:00