Abstract:
:We found that the presence of plasmids expressing tetracycline resistance or chloramphenicol resistance genes, but not those expressing ampicillin resistance or kanamycin resistance genes, in Escherichia coli led to the retardation of the process of removal of the heat-aggregated proteins (i.e. the S fraction) from the bacterial cells. The presence of chloramphenicol acetyltransferase in the S fraction is demonstrated. Moreover, we observed that the expression of T7 RNA polymerase gene had an influence on S fraction removal. These results suggest that high level production of some heterologous proteins which are accumulated in the cytoplasm, but not proteins exported through the cell membranes, may cause overloading of the S fraction and delay in the removal of heat-aggregated proteins from bacterial cells.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Kedzierska S,Staniszewska M,Potrykus J,Wegrzyn Gdoi
10.1111/j.1574-6968.1999.tb13673.xkeywords:
subject
Has Abstractpub_date
1999-07-15 00:00:00pages
279-84issue
2eissn
0378-1097issn
1574-6968pii
S0378109799002451journal_volume
176pub_type
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