Specific mutations in the Mycobacterium tuberculosis rpoB gene are associated with increased dnaE2 expression.

Abstract:

:In Mycobacterium tuberculosis (MTB), rifampicin resistance is almost invariably due to mutations in the rpoB gene, whose function is critical for cell viability. Most of these mutations, at least initially, impair the fitness of the bacteria but confer a selective advantage when antibiotic pressure is exerted. Subsequent adaptation may be critical to restore fitness. The possibility was considered that MTB with mutations in the rpoB gene elicits a constitutive stress response, increasing the probability of subsequent adaptation. In order to test this hypothesis, the expression of recA and dnaE2, an inducible putative error-prone DNA polymerase, was determined in six different isogenic laboratory-generated rpoB-mutants of MTB. Expression levels were determined with real-time PCR and the data obtained were compared with those of the wild-type parent. In four of the six rpoB mutants, a two- to fivefold induction of dnaE2 was detected (P<0.05). Thus, the presence of specific mutations in rpoB is not only associated with impaired fitness but also results in a detectable, moderate yet persistent increase in the expression of dnaE2 but not recA.

journal_name

FEMS Microbiol Lett

authors

Bergval IL,Klatser PR,Schuitema AR,Oskam L,Anthony RM

doi

10.1111/j.1574-6968.2007.00905.x

subject

Has Abstract

pub_date

2007-10-01 00:00:00

pages

338-43

issue

2

eissn

0378-1097

issn

1574-6968

pii

FML905

journal_volume

275

pub_type

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