Abstract:
:Recent reports have indicated that cholesterol plays a crucial role during the uptake of mycobacteria by macrophages. However, the significance of cholesterol modification enzymes encoded by Mycobacterium tuberculosis for bacterial pathogenicity remains unknown. Here, the authors explored whether the well-known cholesterol modification enzyme, cholesterol oxidase (ChoD), is important for virulence of the tubercle bacillus. Homologous recombination was used to replace the choD gene from the M. tuberculosis genome with a nonfunctional copy. The resultant mutant (delta choD) was attenuated in peritoneal macrophages. No attenuation in macrophages was observed when the same strain was complemented with an intact choD gene controlled by a heat shock promoter (delta choDP(hsp)choD). The mice infection experiments confirm the significance of ChoD in the pathogenesis of M. tuberculosis.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Brzostek A,Dziadek B,Rumijowska-Galewicz A,Pawelczyk J,Dziadek Jdoi
10.1111/j.1574-6968.2007.00865.xsubject
Has Abstractpub_date
2007-10-01 00:00:00pages
106-12issue
1eissn
0378-1097issn
1574-6968pii
FML865journal_volume
275pub_type
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