Cholesterol oxidase is required for virulence of Mycobacterium tuberculosis.

Abstract:

:Recent reports have indicated that cholesterol plays a crucial role during the uptake of mycobacteria by macrophages. However, the significance of cholesterol modification enzymes encoded by Mycobacterium tuberculosis for bacterial pathogenicity remains unknown. Here, the authors explored whether the well-known cholesterol modification enzyme, cholesterol oxidase (ChoD), is important for virulence of the tubercle bacillus. Homologous recombination was used to replace the choD gene from the M. tuberculosis genome with a nonfunctional copy. The resultant mutant (delta choD) was attenuated in peritoneal macrophages. No attenuation in macrophages was observed when the same strain was complemented with an intact choD gene controlled by a heat shock promoter (delta choDP(hsp)choD). The mice infection experiments confirm the significance of ChoD in the pathogenesis of M. tuberculosis.

journal_name

FEMS Microbiol Lett

authors

Brzostek A,Dziadek B,Rumijowska-Galewicz A,Pawelczyk J,Dziadek J

doi

10.1111/j.1574-6968.2007.00865.x

subject

Has Abstract

pub_date

2007-10-01 00:00:00

pages

106-12

issue

1

eissn

0378-1097

issn

1574-6968

pii

FML865

journal_volume

275

pub_type

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