Plasmid analysis and antimicrobial susceptibilities of Peptostreptococcus species.

Abstract:

:There are no published methods on plasmid isolation from Peptostreptococcus spp., therefore two methods of plasmid isolation from this genera were analysed: the boiling and alkaline-SDS methods. Plasmid DNA was not recovered by the boiling method, however, with the alkaline-SDS method, cryptic plasmid DNA was detected in two P. asaccharolyticus and one P. magnus strains. To achieve optimum lysis, Peptostreptococcus cells were treated with lysozyme (2 mg/ml) for 15 min. at 37 degrees C followed by proteinase K (0.2 mg/ml) for 1 h at 37 degrees C. In addition we report, the occurrence of clindamycin or metronidazole-resistant peptostreptococci, but these phenotypes were not correlated with plasmid carriage.

journal_name

FEMS Microbiol Lett

authors

Ng LK,Dillon JA

doi

10.1016/0378-1097(89)90168-7

subject

Has Abstract

pub_date

1989-10-01 00:00:00

pages

47-51

issue

1-2

eissn

0378-1097

issn

1574-6968

journal_volume

52

pub_type

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