Abstract:
:Divergicin 750, a bacteriocin produced by Carnobacterium divergens 750, preferentially inhibited the growth of strains of Carnobacterium and Enterococcus. Selected strains of Listeria monocytogenes and Clostridium perfringens were also inhibited. The bacteriocin was purified to homogeneity by ammonium sulfate precipitation and sequential S-Sepharose, hydrophobic interaction and reversed phase chromatography. The complete amino acid sequence was determined by Edman degradation. The peptide consisted of 34 amino acid residues. The calculated M(r) from the peptide sequence, 3447.7, agreed well with that obtained by mass spectrometry. Divergicin 750 did not show any sequence similarities to other known bacteriocins. The plasmid-located structural gene encoding divergicin 750 (dvn750) was cloned and sequenced. The gene encoded a primary translation product of 63 amino acids with a deduced M(r) = 6789.4 which is cleaved between amino acid residues 29 and 30 to yield the mature bacteriocin.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Holck A,Axelsson L,Schillinger Udoi
10.1111/j.1574-6968.1996.tb08043.xsubject
Has Abstractpub_date
1996-02-15 00:00:00pages
163-8issue
2eissn
0378-1097issn
1574-6968pii
0378109795005005journal_volume
136pub_type
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