Abstract:
:The plant-growth promoting rhizobacterium Azospirillum lipoferum strain 4B generates in vitro a stable phase variant designated 4VI at frequencies of 10(-4) to 10(-3) per cell per generation. Variant 4VI displays pleitropic modifications, such as the loss of swimming motility and the inability to assimilate certain sugars compared to the wild type. The mechanism underlying phase variation is unknown. To determine whether RecA-mediated processes are involved in phase variation, the recA gene of A. lipoferum 4B was cloned and sequenced and a recA mutant (termed 4BrecA) was constructed by allelic exchange. Strain 4BrecA showed increased sensitivity to UV and MMS compared with 4B and impaired recombinase activity. The ability to generate variants in vitro was not altered; the variants from 4BrecA exhibited all morphological and biochemical features characteristic of the variant generated by strain 4B. However, the frequency of variants generated by 4BrecA was increased by up to 10-fold. So, in contrast with many studies showing the abolition or a large reduction of the frequency of phase variation in recA mutants, this study describes an enhancement of phase variation in the absence of a functional recA.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Vial L,Pothier JF,Normand P,Moënne-Loccoz Y,Bally R,Wisniewski-Dyé Fdoi
10.1016/j.femsle.2004.05.052keywords:
subject
Has Abstractpub_date
2004-07-15 00:00:00pages
291-9issue
2eissn
0378-1097issn
1574-6968pii
S0378109704004057journal_volume
236pub_type
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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