Fine structure of hepatitis B virus surface antigen produced by recombinant yeast: comparison with HBsAg of human origin.

Abstract:

:The ultrastructure of hepatitis B virus surface antigen (HBsAg) particles produced by recombinant yeast cells was examined using high-resolution negative staining, and ice embedding, electron microscopy. With negative staining, the HBsAg particles were spherical to slightly ovoid with a mean diameter of 27.5 nm and consisted of many subunits each 4 nm in diameter. Subunits were marked with a minute central pore. With ice embedding, particles were mostly spherical to ovoid, with a mean diameter of 23.7 nm and a 7-8 nm thick cortex surrounding an electron translucent core. Human HBsAg particles, examined using the same methods, were smaller, apparently because of molecular differences in polypeptide structure.

journal_name

FEMS Microbiol Lett

authors

Yamaguchi M,Sugahara K,Shiosaki K,Mizokami H,Takeo K

doi

10.1111/j.1574-6968.1998.tb13171.x

subject

Has Abstract

pub_date

1998-08-15 00:00:00

pages

363-7

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(98)00303-6

journal_volume

165

pub_type

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