Abstract:
:Multidrug efflux pumps contribute to multiple antibiotic resistance in Pseudomonas aeruginosa. Pump expression usually has been quantified by Western blotting. Quantitative real-time polymerase chain reaction has been developed to measure mRNA expression for genes of interest. Whether this method correlates with pump protein quantities is unclear. We devised a real-time PCR for mRNA expression of MexAB-OprM and MexXY-OprM multidrug efflux pumps. In laboratory strains differing in MexB and MexY expression and in several clinical isolates, protein and mRNA expression correlated well. Quantitative real-time PCR should be a useful alternative in quantitating expression of multidrug efflux pumps by P. aeruginosa isolates in clinical laboratories.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Yoneda K,Chikumi H,Murata T,Gotoh N,Yamamoto H,Fujiwara H,Nishino T,Shimizu Edoi
10.1016/j.femsle.2004.11.048keywords:
subject
Has Abstractpub_date
2005-02-01 00:00:00pages
125-31issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(04)00881-Xjournal_volume
243pub_type
杂志文章abstract::The degradation of a series of nitroaromatic compounds by the lignin-degrading fungus Phanerochaete chrysosporium was examined. From 4-nitrotoluene (4-NT), several metabolic intermediates were identified. Initially, 4-NT was converted to 4-nitrobenzyl alcohol (4-NBA), followed by the oxidation reactions to form 4-nitr...
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journal_title:FEMS microbiology letters
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更新日期:2020-02-01 00:00:00