Water table related variations in the abundance of intact archaeal membrane lipids in a Swedish peat bog.

abstract：:The ultrastructure of hepatitis B virus surface antigen (HBsAg) particles produced by recombinant yeast cells was examined using high-resolution negative staining, and ice embedding, electron microscopy. With negative staining, the HBsAg particles were spherical to slightly ovoid with a mean diameter of 27.5 nm and co...

journal_title：FEMS microbiology letters

authors： Yamaguchi M,Sugahara K,Shiosaki K,Mizokami H,Takeo K

更新日期：1998-08-15 00:00:00

abstract：:A class 1 integron was detected in a single multidrug-resistant strain of enterotoxigenice Escherichia coli (ETEC) O159 after examination of 23 clinical E. coli isolates. This isolate was resistant to streptomycin, kanamycin, gentamicin, chloramphenicol and ampicillin. Sequencing of the class 1 integron identified thr...

journal_title：FEMS microbiology letters

authors： Ahmed AM,Shimamoto T

更新日期：2004-06-15 00:00:00

abstract：:Acetobacter spp. are used for industrial vinegar production because of their high ability to oxidize ethanol to acetic acid and high resistance to acetic acid. Two-dimensional gel electrophoretic analysis of a soluble fraction of Acetobacter aceti revealed the presence of several proteins whose production was enhanced...

journal_title：FEMS microbiology letters

authors： Nakano S,Fukaya M,Horinouchi S

更新日期：2004-06-15 00:00:00

abstract：:Lyme disease is a multisystemic disorder caused by Borrelia burgdorferi, an invasive spirochete. B. burgdorferi has a predilection for collagenous tissue and one major clinical manifestation of the disease is arthritis. We have identified a collagenolytic activity in B. burgdorferi detergent lysates using iodinated ge...

journal_title：FEMS microbiology letters

authors： Grab DJ,Kennedy R,Philipp MT

更新日期：1996-10-15 00:00:00

abstract：:The frequency of spontaneous as well as induced reversions of auxotrophic mutations in Escherichia coli AB1157 and its DeltadnaK and DeltadnaKdnaJ derivatives was estimated. The obtained results demonstrate that both mutants tested are characterized by elevated frequency of spontaneous reversions compared to their AB1...

journal_title：FEMS microbiology letters

authors： Grudniak AM,Kuć M,Wolska KI

更新日期：2005-01-15 00:00:00

abstract：:Halobacillus trueperi accumulates glycine betaine under condition of high osmolarity. A fragment of the glycine betaine transporter betH gene was obtained from the genome of H. trueperi with degenerate primers. Through Southern blot hybridization and inverse PCR, a 5.1 kb EcoRI fragment containing the complete betH ge...

journal_title：FEMS microbiology letters

authors： Lu W,Zhao B,Feng D,Yang S

更新日期：2004-06-15 00:00:00

abstract：:We describe a new procedure allowing the generation and detection of immunogenic antigens from Helicobacter pylori via the hemolysin secretion apparatus of Escherichia coli. The gene (or gene fragment) encoding the H. pylori protein (or protein domain) is inserted in-frame into a residual portion of the hemolysin gene...

journal_title：FEMS microbiology letters

authors： Spreng S,Gentschev I,Goebel W,Mollenkopf H,Eck M,Müller-Hermelink HK,Schmausser B

更新日期：2000-05-15 00:00:00

abstract：:In our recent screen for soil-induced genes, the expression of andA operon (andAcAdAbAa) for anthranilate catabolism in Burkholderia multivorans ATCC 17616 was found to increase dramatically in a soil sample (Nishiyama et al., Environ Microbiol 12: 2539, 2010). The operon was preceded by andR encoding a putative trans...

journal_title：FEMS microbiology letters

authors： Nishiyama E,Ohtsubo Y,Yamamoto Y,Nagata Y,Tsuda M

更新日期：2012-05-01 00:00:00

abstract：:The role in virulence of Actinobacillus pleuropneumoniae urease activity was investigated. A urease-negative mutant was isolated following transposon mutagenesis with a mini-Tn10 derivative. Both the parent strain and the urease-negative mutant exhibited identical LD50 values in a murine infection model. Pig challenge...

journal_title：FEMS microbiology letters

authors： Tascón Cabrero RI,Vázquez-Boland JA,Gutiérrez CB,Rodríguez-Barbosa JI,Rodríguez-Ferri EF

更新日期：1997-03-01 00:00:00

abstract：:Cyanobacterial natural products offer new possibilities for drugs and lead compounds but many factors can inhibit the production of sufficient yields for pharmaceutical processes. While Escherichia coli and Streptomyces sp. have been used as heterologous expression hosts to produce cyanobacterial natural products, the...

journal_title：FEMS microbiology letters

authors： Wells KN,Videau P,Nelson D,Eiting JE,Philmus B

更新日期：2018-08-01 00:00:00

abstract：:We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to...

journal_title：FEMS microbiology letters

authors： Devillard E,Newbold CJ,Scott KP,Forano E,Wallace RJ,Jouany JP,Flint HJ

更新日期：1999-12-01 00:00:00

abstract：:The identification of virulence factors in Rhodococcus equi has been severely hampered by the lack of a method for in vivo random insertion mutagenesis. This study reports the use of transposomes to generate random insertions of a gene conferring kanamycin resistance into the genome of R. equi ATCC 33701. Southern hyb...

journal_title：FEMS microbiology letters

authors： Mangan MW,Meijer WG

更新日期：2001-12-18 00:00:00

abstract：:A transformation system for Moorella thermoacetica ATCC39073 was developed using thermostable kanamycin resistant gene (kanR) derived from the plasmid pJH1 that Streptococcus faecalis harbored. When kanR with its native promoter was introduced into uracil auxotrophic mutant of M. thermoacetica ATCC39073 together with ...

journal_title：FEMS microbiology letters

authors： Iwasaki Y,Kita A,Sakai S,Takaoka K,Yano S,Tajima T,Kato J,Nishio N,Murakami K,Nakashimada Y

更新日期：2013-06-01 00:00:00

abstract：:To improve expression efficiency of the leuB gene of an extreme thermophile. Thermus thermophilus, in Escherichia coli, the gene was placed under a potent promoter, tac. However, the expression was hardly improved, despite increased transcription. The expression under tac promoter was significantly improved by introdu...

journal_title：FEMS microbiology letters

authors： Ishida M,Oshima T

更新日期：1996-01-01 00:00:00

abstract：:The PenJ protein of the penicillinase gene (penP) expression system from Bacillus licheniformis is an antirepressor and membrane receptor for beta-lactam antibiotics. A putative beta-lactam antibiotic binding site including Ser402 and Lys405, which are homologous to the conserved sequence for the beta-lactam binding s...

journal_title：FEMS microbiology letters

authors： Takagi M,Ohta T,Johki S,Imanaka T

更新日期：1993-06-01 00:00:00

abstract：:Efficient type-III secretion depends on cytosolic molecular chaperones, which bind specifically to the translocators and effectors. In the past there has been a tendency to shoe-horn all type-III-secretion chaperones into a single structural and functional class. However, we have shown that the LcrH/SycD-like chaperon...

journal_title：FEMS microbiology letters

authors： Pallen MJ,Francis MS,Fütterer K

更新日期：2003-06-06 00:00:00

abstract：:The coiled-coil protein DivIVA is a determinant of apical growth and hyphal branching in Streptomyces coelicolor. We have investigated the properties of this protein and the involvement of different domains in its essential function and subcellular targeting. In S. coelicolor cell extracts, DivIVA was present as large...

journal_title：FEMS microbiology letters

authors： Wang SB,Cantlay S,Nordberg N,Letek M,Gil JA,Flärdh K

更新日期：2009-08-01 00:00:00

abstract：:Despite the large number of techniques available for the transformation of bacteria, several species are still resistant to the introduction of foreign DNA. Oenococcus oeni are among the organisms that are particularly refractory to transformation. However, conjugal experiments from Lactococcus lactis to O. oeni with ...

journal_title：FEMS microbiology letters

authors： Beltramo C,Oraby M,Bourel G,Garmyn D,Guzzo J

更新日期：2004-07-01 00:00:00

abstract：:The study compared images of mature Streptococcus mutans biofilms captured at increasing magnification to determine which microscopy method is most acceptable for imaging the biofilm topography and the extracellular polymeric substance (EPS). In vitro S. mutans biofilms were imaged using (1) scanning electron microsco...

journal_title：FEMS microbiology letters

authors： Weber K,Delben J,Bromage TG,Duarte S

更新日期：2014-01-01 00:00:00

abstract：:Use of the green fluorescent protein (GFP) of Aequorea victoria as a reporter for protein and DNA localization has provided sensitive, new approaches for studying the organization of the bacterial cell, leading to new insights into diverse cellular processes. GFP has many characteristics that make it useful for locali...

journal_title：FEMS microbiology letters

authors： Phillips GJ

更新日期：2001-10-16 00:00:00

abstract：:The filamentous fungus Fusarium lateritium is cyanide tolerant, due partly to the induction of the enzyme cyanide hydratase in the presence of cyanide. This enzyme catalyses the hydration of cyanide to formamide. The expression in Escherichia coli of a cDNA clone encoding cyanide hydratase is described. The cDNA clone...

journal_title：FEMS microbiology letters

authors： Brown DT,Turner PD,O'Reilly C

更新日期：1995-12-15 00:00:00

abstract：:The Escherichia coli yehZYXW operon encodes a putative osmoprotectant uptake system of the ABC transporter family. yehZ is identical to osmF, an osmotically inducible gene identified previously. Construction and analysis of a yehZ-lacZ transcriptional fusion demonstrated that yehZ is inducible not only by osmolarity, ...

journal_title：FEMS microbiology letters

authors： Checroun C,Gutierrez C

更新日期：2004-07-15 00:00:00

abstract：:The major haem component in the black pigment of Porphyromonas gingivalis is the mu-oxo bishaem of iron protoporphyrin IX and formation and cell-surface binding of this haem species is proposed as an extracellular buffer against reactive oxidants [Smalley, J.W. et al. (1998) Biochem. J. 331, 681-685]. P. gingivalis ce...

journal_title：FEMS microbiology letters

authors： Smalley JW,Birss AJ,Silver J

更新日期：2000-02-01 00:00:00

abstract：:Bacillus subtilis possesses two isogenes encoding glutamate racemases, the poly-gamma-glutamate synthesis-linking Glr enzyme and the YrpC isozyme, and produces abundant amounts of the Glr enzyme. The YrpC isozyme, but not the Glr enzyme, was found to influence the activity of DNA gyrase, as did the MurI-type glutamate...

journal_title：FEMS microbiology letters

authors： Ashiuchi M,Kuwana E,Komatsu K,Soda K,Misono H

更新日期：2003-06-27 00:00:00

abstract：:A novel strain of Rhizopus oryzae WPG secretes a noninduced lipase (ROLw) in the culture medium; purified ROLw is a protein of 29 kDa, the 45 N-terminal amino acid residues were sequenced, this sequence is very homologous to Rhizopus delemar lipase (RDL), Rhizopus niveus lipase (RNL) and R. oryzae lipase (ROL29) seque...

journal_title：FEMS microbiology letters

authors： Salah RB,Mosbah H,Fendri A,Gargouri A,Gargouri Y,Mejdoub H

更新日期：2006-07-01 00:00:00

abstract：:Two cDNAs encoding feedback inhibition-insensitive serine acetyltransferases of Arabidopsis thaliana were expressed in the chromosomal serine acetyltransferase-deficient and L-cysteine non-utilizing Escherichia coli strain JM39-8. The transformants produced 1600 to 1700 mg l(-1) of L-cysteine and L-cystine from glucos...

journal_title：FEMS microbiology letters

authors： Takagi H,Awano N,Kobayashi S,Noji M,Saito K,Nakamori S

更新日期：1999-10-15 00:00:00

abstract：:Comparisons of growth and surface colonisation of Enterococcus faecium L-forms and their cell-walled forms were undertaken to produce information about their ability to form sessile cells. The growth of L-forms in liquid culture was slower than that of the parent. This was reflected in their longer lag phase and slowe...

journal_title：FEMS microbiology letters

authors： Jass J,Phillips LE,Allan EJ,Costerton JW,Lappin-Scott HM

更新日期：1994-01-15 00:00:00

abstract：:A cryptic plasmid from Arthrobacter rhombi PRH1, designated as pPRH, was sequenced and characterized. It was 5000 bp in length with a G+C content of 66 mol%. The plasmid pPRH was predicted to encode six putative open reading frames (ORFs), in which ORF2 and ORF3 formed the minimal replicon of plasmid pPRH and shared 5...

journal_title：FEMS microbiology letters

authors： Stanislauskiene R,Gasparaviciute R,Vaitekunas J,Meskiene R,Rutkiene R,Casaite V,Meskys R

更新日期：2012-02-01 00:00:00

abstract：:A critical step in the process of metagenome analysis is to screen for clones that contain specific genes among a large number of clones. To form one of the sequence-based screening tools of a metagenome library, we designed a format of microarray [metagenome microarray (MGA)] that is arrayed with fosmid library clone...

journal_title：FEMS microbiology letters

authors： Park SJ,Kang CH,Chae JC,Rhee SK

更新日期：2008-07-01 00:00:00

abstract：:Shigella strains are human pathogens and their identification is usually based on their O-antigens. The O-antigen gene cluster of Shigella boydii O11 was sequenced. All the expected genes for the synthesis of the O-antigen were identified on the basis of homology and genes for the biosynthesis of dTDP-l-Rhamnose, gene...

journal_title：FEMS microbiology letters

authors： Tao J,Feng L,Guo H,Li Y,Wang L

更新日期：2004-05-01 00:00:00