Expression of the cyanide hydratase enzyme from Fusarium lateritium in Escherichia coli and identification of an essential cysteine residue.

Abstract:

:The filamentous fungus Fusarium lateritium is cyanide tolerant, due partly to the induction of the enzyme cyanide hydratase in the presence of cyanide. This enzyme catalyses the hydration of cyanide to formamide. The expression in Escherichia coli of a cDNA clone encoding cyanide hydratase is described. The cDNA cloned was expressed as a transcriptional fusion in the expression vector pKK233-2 and a high level of activity of cyanide hydratase was detected in E. coli. Site-directed mutagenesis of the cys-163 residue inactivated the enzyme.

journal_name

FEMS Microbiol Lett

authors

Brown DT,Turner PD,O'Reilly C

doi

10.1111/j.1574-6968.1995.tb07928.x

subject

Has Abstract

pub_date

1995-12-15 00:00:00

pages

143-6

issue

2-3

eissn

0378-1097

issn

1574-6968

journal_volume

134

pub_type

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