Abstract:
:The filamentous fungus Fusarium lateritium is cyanide tolerant, due partly to the induction of the enzyme cyanide hydratase in the presence of cyanide. This enzyme catalyses the hydration of cyanide to formamide. The expression in Escherichia coli of a cDNA clone encoding cyanide hydratase is described. The cDNA cloned was expressed as a transcriptional fusion in the expression vector pKK233-2 and a high level of activity of cyanide hydratase was detected in E. coli. Site-directed mutagenesis of the cys-163 residue inactivated the enzyme.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Brown DT,Turner PD,O'Reilly Cdoi
10.1111/j.1574-6968.1995.tb07928.xsubject
Has Abstractpub_date
1995-12-15 00:00:00pages
143-6issue
2-3eissn
0378-1097issn
1574-6968journal_volume
134pub_type
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