Expression of the cyanide hydratase enzyme from Fusarium lateritium in Escherichia coli and identification of an essential cysteine residue.


:The filamentous fungus Fusarium lateritium is cyanide tolerant, due partly to the induction of the enzyme cyanide hydratase in the presence of cyanide. This enzyme catalyses the hydration of cyanide to formamide. The expression in Escherichia coli of a cDNA clone encoding cyanide hydratase is described. The cDNA cloned was expressed as a transcriptional fusion in the expression vector pKK233-2 and a high level of activity of cyanide hydratase was detected in E. coli. Site-directed mutagenesis of the cys-163 residue inactivated the enzyme.


FEMS Microbiol Lett


Brown DT,Turner PD,O'Reilly C




Has Abstract


1995-12-15 00:00:00












  • Clonal relations of atypical enteropathogenic Escherichia coli O157:H16 strains isolated from various sources from several countries.

    abstract::Atypical enteropathogenic Escherichia coli (aEPEC) is comprised of a large heterogeneous group of strains and serotypes that carry the intimin gene (eae) but no other EPEC virulence factors. In a previous study, we examined a few aEPEC strains of O157:H16 serotype from the U.S. and France and found these to be nearly ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Feng PC,Keys C,Lacher DW,Beutin L,Bentancor A,Heuvelink A,Afset JE,Rumi V,Monday S

    更新日期:2012-12-01 00:00:00

  • Proteomics and the stressful life of lactobacilli.

    abstract::Lactobacilli occupy specific ecological niches, where they represent a major component of foods, human and animal microbial communities. Employing these bacteria in industrial fermentations or for human health benefits exposes them to certain life-threatening conditions where their ability to adapt plays a key role in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Hussain MA,Hosseini Nezhad M,Sheng Y,Amoafo O

    更新日期:2013-12-01 00:00:00

  • Efficient cloning in Bacillus megaterium: comparison to Bacillus subtilis and Escherichia coli cloning hosts.

    abstract::Quantitative cloning efficiencies for B. megaterium, B. subtilis, and E. coli were compared. Transformation of B. megaterium is less efficient than transformation of B. subtilis or E. coli. The frequency of recombinant clones was equal in E. coli and B. megaterium; both somewhat higher than in B. subtilis. Equivalent ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Von Tersch MA,Robbins HL

    更新日期:1990-08-01 00:00:00

  • The Brucella abortus vaccine strain B19 carries a deletion in the erythritol catabolic genes.

    abstract::Brucella abortus B19, an avirulent strain obtained by spontaneous mutation, is used worldwide as a vaccine for the control of bovine brucellosis. B19 differs from other B. abortus strains in its sensitivity to erythritol. We took advantage of a previously obtained erythritol sensitive Tn5 insertion mutant of B. abortu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sangari FJ,García-Lobo JM,Agüero J

    更新日期:1994-09-01 00:00:00

  • Molecular characterization of the erythromycin resistance plasmid pPV142 from Staphylococcus simulans.

    abstract::The 2.5-kb erythromycin resistance (EmR) plasmid pPV142 of Staphylococcus simulans 13044 was isolated and characterized. Sequence analysis identified ORF1 and ORF2 encoding a 158-residue replication protein (Rep142) and a 244-residue erythromycin resistance protein (Erm, rRNA adenine N-6-methyltransferase), respective...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Somkuti GA,Solaiman DK,Steinberg DH

    更新日期:1998-08-15 00:00:00

  • Replicating function of the RS1 element associated with Vibrio cholerae CTX phi prophage.

    abstract::The RS1 element associated with Vibrio cholerae CTX phi prophage was cloned from an E1 Tor biotype Vibrio cholerae strain. We used the recA- vaccine strain Peru-15, that lacks the target for RS-mediated site-specific integration, to show that RS1 promotes autonomous replication of a suicide vector. A linker insertion ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Campos J,Fando R,Silva A,Rodriguez BL,Benitez JA

    更新日期:1998-07-01 00:00:00

  • Hydrogenase genes are uncommon and highly conserved in Rhizobium leguminosarum bv. viciae.

    abstract::A screening for hydrogen uptake (hup) genes in Rhizobium leguminosarum bv. viciae isolates from different locations within Spain identified no Hup+ strains, confirming the scarcity of the Hup trait in R. leguminosarum. However, five new Hup+ strains were isolated from Ni-rich soils from Italy and Germany. The hup gene...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fernández D,Toffanin A,Palacios JM,Ruiz-Argüeso T,Imperial J

    更新日期:2005-12-01 00:00:00

  • Molecular characterisation of rough strains of Vibrio cholerae isolated from diarrhoeal cases in India and their comparison to smooth strains.

    abstract::Sixteen of the 18 Vibrio cholerae rough strains isolated from hospitalised diarrhoea patients were found to contain O1 serotype-specific (wbe) genes and all currently known virulence genes. Expression of the regulatory element ToxR was evident in these strains. Cholera toxin production ability of the rough strains was...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: De K,Ramamurthy T,Faruque SM,Yamasaki S,Takeda Y,Nair GB,Nandy RK

    更新日期:2004-03-12 00:00:00

  • Characterization of a novel vip3-type gene from Bacillus thuringiensis and evidence of its presence on a large plasmid.

    abstract::A novel vip3-type gene named vip3LB has been isolated from Bacillus thuringiensis strain BUPM95. The corresponding secreted vegetative insecticidal protein was active against the lepidopteran insect Ephestia kuehniella. The vip3LB gene was shown, for the first time, to be carried by the large plasmid containing the cr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mesrati LA,Tounsi S,Jaoua S

    更新日期:2005-03-15 00:00:00

  • Maricurvus nonylphenolicus gen. nov., sp. nov., a nonylphenol-degrading bacterium isolated from seawater.

    abstract::A novel aerobic, Gram-negative bacterial strain, designated KU41E(T), which degrades p-n-nonylphenol, was isolated from seawater obtained from the coastal region of Ishigaki Island, Japan. Cells are motile, curved rods with a single polar flagellum. Strain KU41E(T) grew at 20-35 °C, pH 7.0-8.0, in the presence of 1.0-...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Iwaki H,Takada K,Hasegawa Y

    更新日期:2012-02-01 00:00:00

  • Fungal cytochrome P450s catalyzing hydroxylation of substituted toluenes to form their hydroxymethyl derivatives.

    abstract::The degradation of a series of nitroaromatic compounds by the lignin-degrading fungus Phanerochaete chrysosporium was examined. From 4-nitrotoluene (4-NT), several metabolic intermediates were identified. Initially, 4-NT was converted to 4-nitrobenzyl alcohol (4-NBA), followed by the oxidation reactions to form 4-nitr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Teramoto H,Tanaka H,Wariishi H

    更新日期:2004-05-15 00:00:00

  • Similar subunit architecture of archaeal and eukaryal RNA polymerases.

    abstract::Protein interactions among RNA polymerase small subunits from the archaeon Methanococcus jannaschii were investigated using affinity pulldown assays in pairwise and higher-order combinations. In the most extensive study of archaeal RNA polymerase subunit interactions to date, including 37 pairs of proteins, 10 ternary...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Best AA,Olsen GJ

    更新日期:2001-02-05 00:00:00

  • Comparison of the inhibitory action on Saccharomyces cerevisiae of weak-acid preservatives, uncouplers, and medium-chain fatty acids.

    abstract::This study was initiated to establish whether inhibition of growth of yeasts by medium-chain fatty acids resembled that caused by weak-acid preservatives or uncouplers. Unlike sorbic acid and 2,4-dinitrophenol, decanoic acid caused rapid cell death at its inhibitory concentration. This suggested a mode of action by me...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Stratford M,Anslow PA

    更新日期:1996-08-15 00:00:00

  • Isolation and sequence analysis of the pmi gene encoding phosphomannose isomerase of Streptococcus mutans.

    abstract::A gene encoding a phosphomannose isomerase from Streptococcus mutans GS-5 was identified immediately downstream from the fructokinase gene, scrK. Nucleotide sequence analysis of this region revealed an open reading frame (ORF) specifying a putative protein of 316 amino acids. The gene cloned in Escherichia coli expres...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sato Y,Yamamoto Y,Kizaki H,Kuramitsu HK

    更新日期:1993-11-15 00:00:00

  • Clonal relationships among Escherichia coli serogroup 06 isolates from human and animal infections.

    abstract::The clonal relationship of thirty E. coli strains of 0 antigen serotype 06 isolated from human, dog, pig or cow infections were investigated. Two main clones with serotypes 06 : H1 or 06 : H31, H- were identified. Isolates from humans, dogs, pigs and cows were found in both clones, indicating that animals are a possib...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cherifi A,Contrepois M,Picard B,Goullet P,Orskov I,Orskov F,De Rycke J

    更新日期:1991-05-15 00:00:00

  • Simple method for cryopreservation of an anaerobic rumen fungus using ethylene glycol and rumen fluid.

    abstract::The cryopreservation of an anaerobic rumen fungus, Piromyces communis OTS1, was examined at -84 degrees C using dimethyl sulfoxide, propylene glycol or ethylene glycol as cryoprotectants. Ethylene glycol was the most effective agent, combining high survival and low toxicity, followed by dimethyl sulfoxide and propylen...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sakurada M,Tsuzuki Y,Morgavi DP,Tomita Y,Onodera R

    更新日期:1995-04-01 00:00:00

  • Characterisation of the bovine enteric calici-like virus, Newbury agent 1.

    abstract::The bovine enteric calici-like virus, Newbury agent 1 (NA1) was characterised to determine if it is a member of the Caliciviridae and to establish its antigenic relationship to the established bovine enteric calicivirus Newbury agent 2 (NA2). Solid phase immune electron microscopy (SPIEM) allowed quantification of NA1...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dastjerdi AM,Snodgrass DR,Bridger JC

    更新日期:2000-11-01 00:00:00

  • Evidence that OmcB and OmpB of Geobacter sulfurreducens are outer membrane surface proteins.

    abstract::The c-type cytochrome (OmcB) and the multicopper protein (OmpB) required for Fe(III) oxide reduction by Geobacter sulfurreducens were predicted previously to be outer membrane proteins, but it is not clear whether they are positioned in a manner that permits the interaction with Fe(III). Treatment of whole cells with ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Qian X,Reguera G,Mester T,Lovley DR

    更新日期:2007-12-01 00:00:00

  • Evidence for inter- and intraspecies biofilm formation variability among a small group of coagulase-negative staphylococci.

    abstract::Coagulase-negative staphylococci (CoNS) are common bacterial colonizers of the human skin. They are often involved in nosocomial infections due to biofilm formation in indwelling medical devices. While biofilm formation has been extensively studied in Staphylococcus epidermidis, little is known regarding other CoNS sp...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Oliveira F,Lima CA,Brás S,França Â,Cerca N

    更新日期:2015-10-01 00:00:00

  • The effect of buthionine sulfoximine on the growth of Leishmania donovani in culture.

    abstract::Changes in composition of the principal low molecular mass thiols of Leishmania donovani were monitored during the transformation of promastigotes, first to stationary phase metacyclic forms and then to amastigotes. No consistent variation in the thiol composition of the parasite which could account for the known incr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Weldrick DP,Chodacka B,Vogt R,Steenkamp DJ

    更新日期:1999-04-01 00:00:00

  • New genus-specific primers for the PCR identification of novel isolates of the genus Streptomonospora.

    abstract::The halophilic actinomycete genus Streptomonospora, forming a distinct branch in the 16S rRNA gene phylogenetic tree adjacent to the genera Nocardiopsis and Thermobifida, was first proposed by Cui et al. to accommodate the species type Streptomonospora salina. During a biodiversity and taxonomic study on halophilic fi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Zhi XY,Tang SK,Li WJ,Xu LH,Jiang CL

    更新日期:2006-10-01 00:00:00

  • Sequence and characterization of an Ehrlichia chaffeensis gene encoding 314 amino acids highly homologous to the NAD A enzyme.

    abstract::DNA sequence analysis of the nadA gene of Ehrlichia chaffeensis revealed a 942 bp open reading frame with the capacity to encode 314 amino acids. The amino acid sequence of the E. chaffeensis quinolinate synthetase A (NAD/A) has 53.6% identity and 82% similarity to the NAD A of the cyanelle of Cyanophora paradoxa. Por...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Yu XJ,Walker DH

    更新日期:1997-09-01 00:00:00

  • Pili of Pasteurella multocida of porcine origin.

    abstract::Using electron microscopy, pili with at least two distinct morphologies were observed on strains of Pasteurella multocida isolated from pigs with atrophic rhinitis. Rigid pili were found on 60-80% of all cells observed. These pili had a strong tendency to lie flat along the side of the outer cell membrane of P. multoc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Isaacson RE,Trigo E

    更新日期:1995-10-15 00:00:00

  • SshAI restriction endonuclease from Salmonella shikmonah.

    abstract::A new type II restriction endonuclease, SshAI, was purified from Salmonella shikmonah TK139 of kangaroo origin. The recognition and cleavage specificity of Ssh AI was determined to be 5'-CC/TNAGG-3', identical to that of SauI from Streptomyces aureofaciens and Bsu36I from Bacillus subtilis. Based on closely related an...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Miyahara M,Nakajima K,Kawanishi T,Mise K

    更新日期:1990-01-01 00:00:00

  • The complex and dynamic genomes of industrial yeasts.

    abstract::The Saccharomyces sensu stricto genus contains many species that are industrially important for fermentation of wines, beers and ales. The molecular characterization of the genomes of yeasts involved in these processes reveals that the majority arose from interspecific hybridization between two and sometimes three yea...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Querol A,Bond U

    更新日期:2009-04-01 00:00:00

  • Internal size variations in Tn1546-like elements due to the presence of IS1216V.

    abstract::In this study, internal size variations in the VanA gene cluster Tn1546, encoding resistance to glycopeptides, is described. Studies of previously uncharacterized size variations of an internal region, encoding the vanX and vanY genes of Tn1546, revealed that these variations were due to the presence of the IS sequenc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jensen LB

    更新日期:1998-12-15 00:00:00

  • Acanthamoeba sp. promotes the survival and growth of Acinetobacter baumanii.

    abstract::Acinetobacter baumanii, which may be found in water, is an important emerging hospital-acquired pathogen. Free-living amoebae can be recovered from the same water networks, and it has been shown that these protozoa may support the growth of other bacteria. In this paper, we have studied potential relationships between...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cateau E,Verdon J,Fernandez B,Hechard Y,Rodier MH

    更新日期:2011-06-01 00:00:00

  • Antibacterial action of acriflavine hydrochloride for eradication of the gastric pathogen Helicobacter pylori.

    abstract::Helicobacter pylori, a type 1 carcinogen, accounts for numerous gastric cancer-related deaths worldwide. Repurposing existing drugs or developing new ones for a combinatorial approach against increasing antimicrobial resistance is the need of the hour. This study highlights the efficacy of acriflavine hydrochloride (A...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Tehlan A,Karmakar BC,Paul S,Kumar R,Kaur I,Ghosh A,Mukhopadhyay AK,Dhar SK

    更新日期:2020-11-23 00:00:00

  • Genetic investigation within Lactococcus garvieae revealed two genomic lineages.

    abstract::The diversity of a collection of 49 Lactococcus garvieae strains, including isolates of dairy, fish, meat, vegetable and cereal origin, was explored using a molecular polyphasic approach comprising PCR-ribotyping, REP and RAPD-PCR analyses and a multilocus restriction typing (MLRT) carried out on six partial genes (at...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Ferrario C,Ricci G,Borgo F,Rollando A,Fortina MG

    更新日期:2012-07-01 00:00:00

  • Unlocking the black box: teaching mathematical modeling with popular culture.

    abstract::Mathematical modeling is an important tool in biological research, allowing for the synthesis of results from many studies into an understanding of a system. Despite this, the need for extensive subject matter knowledge and complex mathematics often leaves modeling as an esoteric subspecialty. A 2-fold approach can be...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lofgren ET

    更新日期:2016-10-01 00:00:00