Expression of the cyanide hydratase enzyme from Fusarium lateritium in Escherichia coli and identification of an essential cysteine residue.


:The filamentous fungus Fusarium lateritium is cyanide tolerant, due partly to the induction of the enzyme cyanide hydratase in the presence of cyanide. This enzyme catalyses the hydration of cyanide to formamide. The expression in Escherichia coli of a cDNA clone encoding cyanide hydratase is described. The cDNA cloned was expressed as a transcriptional fusion in the expression vector pKK233-2 and a high level of activity of cyanide hydratase was detected in E. coli. Site-directed mutagenesis of the cys-163 residue inactivated the enzyme.


FEMS Microbiol Lett


Brown DT,Turner PD,O'Reilly C




Has Abstract


1995-12-15 00:00:00












  • Estimate of the diversity of viral and bacterial assemblage in the coastal water of Lake Baikal.

    abstract::In this study, we analysed the diversity and composition of double-stranded DNA viral and bacterial communities within the sample of surface coastal water of Southern Baikal through metagenomics and deep sequencing of the 16S ribosomal RNA gene, respectively. The 16S rRNA gene analysis has revealed 14 phyla and domina...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Butina TV,Bukin YS,Krasnopeev AS,Belykh OI,Tupikin AE,Kabilov MR,Sakirko МV,Belikov SI

    更新日期:2019-05-01 00:00:00

  • A xylanase produced by the rumen anaerobic protozoan Polyplastron multivesiculatum shows close sequence similarity to family 11 xylanases from gram-positive bacteria.

    abstract::We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Devillard E,Newbold CJ,Scott KP,Forano E,Wallace RJ,Jouany JP,Flint HJ

    更新日期:1999-12-01 00:00:00

  • MHYT, a new integral membrane sensor domain.

    abstract::MHYT, a new conserved protein domain with a likely signaling function, is described. This domain consists of six transmembrane segments, three of which contain conserved methionine, histidine, and tyrosine residues that are projected to lie near the outer face of the cytoplasmic membrane. In Synechocystis sp. PCC6803,...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Galperin MY,Gaidenko TA,Mulkidjanian AY,Nakano M,Price CW

    更新日期:2001-11-27 00:00:00

  • Mechanisms of action of ostrich beta-defensins against Escherichia coli.

    abstract::To understand their mechanism of antimicrobial activity against Gram-negative bacteria, ostrich beta-defensins, ostricacins-1 and 2 (Osp-1 and Osp-2), were compared with those of sheep myeloid antimicrobial peptide (SMAP)-29 and human neutrophil peptide (HNP)-1, well-characterized sheep alpha-helical and human alpha-d...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sugiarto H,Yu PL

    更新日期:2007-05-01 00:00:00

  • Identification and characterization of a cross-reactive and a unique B-cell epitope on the hsp60 homologue from Neisseria gonorrhoeae.

    abstract::Two monoclonal antibodies (G6 and 7B), generated against a 63-kDa stress protein (GSP63) from Neisseria gonorrhoeae strain VP1, were used to investigate the antigenic heterogeneity of GSP63 among the Neisseriaceae and its antigenic relationship with the Hsp60 heat-shock protein family. Immunoblotting experiments demon...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Pannekoek Y,Dankert J,van Putten JP

    更新日期:1992-11-15 00:00:00

  • Comparison of the inhibitory action on Saccharomyces cerevisiae of weak-acid preservatives, uncouplers, and medium-chain fatty acids.

    abstract::This study was initiated to establish whether inhibition of growth of yeasts by medium-chain fatty acids resembled that caused by weak-acid preservatives or uncouplers. Unlike sorbic acid and 2,4-dinitrophenol, decanoic acid caused rapid cell death at its inhibitory concentration. This suggested a mode of action by me...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Stratford M,Anslow PA

    更新日期:1996-08-15 00:00:00

  • Genetic analysis of CTX prophages with special reference to ctxB and rstR alleles of Vibrio cholerae O139 strains isolated from Kolkata over a decade.

    abstract::Chronological analysis of 125 Vibrio cholerae O139 strains isolated during 1993-2005 in Kolkata revealed the prevalence of two new genotypes of cholera toxin (CT) and novel combinations of ctxB and rstR alleles resulting in variant CTX prophages. One of the new genotypes of ctxB, which first appeared in 1996 with the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Raychoudhuri A,Mukherjee P,Ramamurthy T,Nandy RK,Takeda Y,Nair GB,Mukhopadhyay AK

    更新日期:2010-02-01 00:00:00

  • Transcriptional repressor Rex is involved in regulation of oxidative stress response and biofilm formation by Streptococcus mutans.

    abstract::The transcriptional repressor Rex has been implicated in the regulation of energy metabolism and fermentative growth in response to redox potential. Streptococcus mutans, the primary causative agent of human dental caries, possesses a gene that encodes a protein with high similarity to members of the Rex family of pro...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bitoun JP,Nguyen AH,Fan Y,Burne RA,Wen ZT

    更新日期:2011-07-01 00:00:00

  • Biotransformation of trinitrotoluene by Citrobacter sp. YC4 and evaluation of its cyto-toxicological effects.

    abstract::Trinitrotoluene (TNT) is an explosive chemical generally used for military, civil and industrial purposes. Therefore, TNT residue can be found in soil and ground water as an environmental pollutant. The environmental control of TNT pollution has become a critical issue because of its potential toxicity and carcinogeni...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kao CM,Wei SF,Chen SC,Yao CL,Ma C,Chien CC

    更新日期:2018-01-01 00:00:00

  • The triazine hydrolase gene trzN from Nocardioides sp. strain C190: cloning and construction of gene-specific primers.

    abstract::Using oligonucleotides derived from the N-terminal sequence of a triazine hydrolase from Nocardioides sp. strain C190, two DNA fragments containing trzN were cloned into Escherichia coli and their nucleotide sequences were determined. The 456-amino acid polypeptide predicted from the 1356-bp trzN ORF displayed signifi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mulbry WW,Zhu H,Nour SM,Topp E

    更新日期:2002-01-02 00:00:00

  • Sequence of the gene encoding type F neurotoxin of Clostridium botulinum.

    abstract::Primers designed to conserved regions of botulinum and tetanus clostridial toxins were used to amplify DNA fragments from non-proteolytic Clostridium botulinum type F (202F) DNA using polymerase chain reaction technology. The fragments were cloned and the complete nucleotide sequence of the gene encoding type F toxin ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: East AK,Richardson PT,Allaway D,Collins MD,Roberts TA,Thompson DE

    更新日期:1992-09-15 00:00:00

  • Close relationship of RNase P RNA in Gemmata and anammox planctomycete bacteria.

    abstract::The relationship of RNase P RNA from anammox bacteria 'Candidatus Brocadia anammoxidans' and 'Candidatus Kuenenia stuttgartiensis' with that from other Planctomycetes was investigated. Newly identified rnpB gene sequences were aligned against existing planctomycete RNase P RNA sequences and secondary structures deduce...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Butler MK,Op den Camp HJ,Harhangi HR,Lafi FF,Strous M,Fuerst JA

    更新日期:2007-03-01 00:00:00

  • Production of unmarked mutations in mycobacteria using site-specific recombination.

    abstract::Gene disruption experiments play an important role in the functional characterization of genes in mycobacteria and rely mostly on the use of one or two antibiotic resistance markers. We have developed a system for mycobacteria which features both the advantages of the use of antibiotic resistance markers for gene disr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Malaga W,Perez E,Guilhot C

    更新日期:2003-02-28 00:00:00

  • Differential expression of proteolytic enzymes in endosymbiont-harboring Crithidia species.

    abstract::Crithidia oncopelti, Crithidia deanei and Crithidia desouzai are flagellates of the Trypanosomatidae family that present bacterium-like endosymbionts in their cytoplasm. Gelatin-SDS-PAGE analysis was used to characterize cell-associated and extracellular proteinases in these organisms. Our survey indicates that the pr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: d'Avila-Levy CM,Melo AC,Vermelho AB,Branquinha MH

    更新日期:2001-08-07 00:00:00

  • Evidence for 1,8-dihydroxynaphthalene melanin in three halophilic black yeasts grown under saline and non-saline conditions.

    abstract::The ascomycetous black yeasts Hortaea werneckii, Phaeotheca triangularis, and Trimmatostroma salinum are halophilic fungi that inhabit hypersaline water of solar salterns. They are characterized by slow, meristematic growth and very thick, darkly pigmented cell walls. The dark pigment, generally thought to be melanin,...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kogej T,Wheeler MH,Lanisnik Rizner T,Gunde-Cimerman N

    更新日期:2004-03-19 00:00:00

  • Double-stranded RNA and virus-like particles in the edible basidiomycete Flammulina velutipes (Enokitake).

    abstract::One of the commercial strains of Flammulina velutipes was analyzed for the presence of double-stranded RNA (dsRNA) elements to examine the underlying mechanism of strain degeneration. As a result, two dsRNA elements sized 1.9 and 1.8 kb were detected in mycelium derived from spontaneously brown-colored fruit body. The...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Magae Y,Hayashi N

    更新日期:1999-11-15 00:00:00

  • Positively regulated expression of the Escherichia coli araBAD promoter in Corynebacterium glutamicum.

    abstract::In Corynebacterium glutamicum the promoter of the araBAD Escherichia coli gene is positively regulated by both arabinose and the araC gene product, as it is the case in the natural host. If the L-arabinose inducer and an active araC gene are present, significant amounts of araBAD promoter expression take place in the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ben-Samoun K,Leblon G,Reyes O

    更新日期:1999-05-01 00:00:00

  • Minocycline resistance in an oral Streptococcus infantis isolate is encoded by tet(S) on a novel small, low copy number plasmid.

    abstract::We have determined the genetic basis of minocycline resistance in a strain of Streptococcus infantis isolated from a healthy human oral cavity. We demonstrate that tet(S), identical to tet(S) found on the enterococcal conjugative transposon Tn6000, is responsible for the observed resistance. The gene is located on a s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ciric L,Brouwer MS,Mullany P,Roberts AP

    更新日期:2014-04-01 00:00:00

  • Impact of oleic acid (cis-9-octadecenoic acid) on bacterial viability and biofilm production in Staphylococcus aureus.

    abstract::Staphylococcus aureus is responsible for a broad variety of chronic infections. Most S. aureus clinical isolates show the capacity to adhere to abiotic surfaces and to develop biofilms. Because S. aureus growing in a biofilm is highly refractory to treatment, inhibition of biofilm formation represents a major therapeu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Stenz L,François P,Fischer A,Huyghe A,Tangomo M,Hernandez D,Cassat J,Linder P,Schrenzel J

    更新日期:2008-10-01 00:00:00

  • Bacillus cereus immune escape: a journey within macrophages.

    abstract::During bacterial infection, professional phagocytes are attracted to the site of infection, where they constitute a first line of host cell defense. Their function is to engulf and destroy the pathogens. Thus, bacteria must withstand the bactericidal activity of professional phagocytes, including macrophages to counte...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Tran SL,Ramarao N

    更新日期:2013-10-01 00:00:00

  • Apparent growth phase-dependent phosphorylation of malonyl coenzyme A:acyl carrier protein transacylase (MCAT), a major fatty acid synthase II component in Mycobacterium bovis BCG.

    abstract::Probing protein extracts from exponentially growing and stationary phase cultures of Mycobacterium bovis BCG with anti-phospho amino acid antibodies revealed a 31-kDa anti-phospho threonine antibody-reactive protein specific to growing culture. The corresponding protein was purified via two-dimensional gel electrophor...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sinha I,Boon C,Dick T

    更新日期:2003-10-10 00:00:00

  • Simplified characterization through site-specific protease-mediated release of affinity proteins selected by staphylococcal display.

    abstract::The production of candidate affinity proteins in a soluble form, for downstream characterization, is often a time-consuming step in combinatorial protein engineering methods. Here, a novel approach for efficient production of candidate clones is described based on direct cleavage of the affinity protein from the surfa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kronqvist N,Löfblom J,Severa D,Ståhl S,Wernérus H

    更新日期:2008-01-01 00:00:00

  • The Aspergillus niger acuA and acuB genes correspond to the facA and facB genes in Aspergillus nidulans.

    abstract::Mutants in Aspergillus niger unable to grow on acetate as a sole carbon source were previously isolated by resistance to 1.2% propionate medium containing 0.1% glucose. AcuA mutants lacked acetyl-CoA synthetase (ACS) activity and acuB mutants lacked both ACS and isocitrate lyase activity. An acuA mutant was transforme...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Papadopoulou S,Sealy-Lewis HM

    更新日期:1999-09-01 00:00:00

  • Metabolic engineering of Escherichia coli for the production of isoprenoids.

    abstract::Metabolic engineering is the practice of using directed genetic manipulations to rewire cellular metabolism primarily with the aim to transform the organism into a single-celled chemical factory. Using biological processes, we can produce more complex chemicals in a more sustainable way. This is particularly important...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Ward VCA,Chatzivasileiou AO,Stephanopoulos G

    更新日期:2018-05-01 00:00:00

  • Assembly of the CotSA coat protein into spores requires CotS in Bacillus subtilis.

    abstract::The CotSA protein, encoded by cotSA (ytxN) of Bacillus subtilis, was detected from the cells at 5 h after the onset of sporulation (T5) and in the spore coat of wild-type cells, but not in cotE, cotS, gerE, or cotSA mutant spores. CotSA was also detected in the sporangium at T5 to T7 but not in the sporangium at T18 o...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Takamatsu H,Kodama T,Watabe K

    更新日期:1999-05-01 00:00:00

  • High correlation between Chagas' disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area.

    abstract::The detection of Trypanosoma cruzi kinetoplast DNA by polymerase chain reaction (PCR) amplification is a potentially powerful tool for the parasitological diagnosis of Chagas' disease. We have applied this technique in a field situation in Bolivia, where 45 children from a primary school were subjected to serological ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wincker P,Bosseno MF,Britto C,Yaksic N,Cardoso MA,Morel CM,Brenière SF

    更新日期:1994-12-15 00:00:00

  • Luminometry and PCR-based monitoring of gene-tagged cyanobacteria in Baltic Sea microcosms.

    abstract::The cyanobacterium Synechocystis 6803 was tagged by chromosomal integration of the firefly luciferase gene, luc, resulting in the modified strain Synechocystis 6803-luc. The tagged cells were monitored in Baltic Sea microcosms both by detection of the luc gene by PCR amplification and by measurement of luc gene expres...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Möller A,Norrby AM,Gustafsson K,Jansson J

    更新日期:1995-06-01 00:00:00

  • Divergicin 750, a novel bacteriocin produced by Carnobacterium divergens 750.

    abstract::Divergicin 750, a bacteriocin produced by Carnobacterium divergens 750, preferentially inhibited the growth of strains of Carnobacterium and Enterococcus. Selected strains of Listeria monocytogenes and Clostridium perfringens were also inhibited. The bacteriocin was purified to homogeneity by ammonium sulfate precipit...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Holck A,Axelsson L,Schillinger U

    更新日期:1996-02-15 00:00:00

  • Specific interaction of the protein-D2 porin of Pseudomonas aeruginosa with antibiotics.

    abstract::The protein-D2 porin of Pseudomonas aeruginosa is lacking in carbapenem or fluoroquinolone-resistant strains and hence was thought to facilitate the diffusion of these antibiotics. We examined the effect of several antibiotics on the single channel conductivity of protein-D2 in planar lipid bilayers and found that flu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ishii J,Nakae T

    更新日期:1996-02-01 00:00:00

  • Ceramide glycosylation and fatty acid hydroxylation influence serological reactivity in Trypanosoma cruzi glycosphingolipids.

    abstract::Ceramide mono (CMH) or dihexoside (CDH) fractions from Trypanosoma cruzi (Dm28c clone) were identified as glucosyl and lactosylceramides containing non-hydroxylated fatty acids. The di-glycosylated form was much more efficiently recognized by sera from T. cruzi-immunized rabbits, indicating that glycosylation influenc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Villas-Boas MH,Wait R,Silva RB,Rodrigues ML,Barreto-Bergter E

    更新日期:2005-03-01 00:00:00