A plasmid-encoded class 1 integron carrying sat, a putative phosphoserine phosphatase gene and aadA2 from enterotoxigenic Escherichia coli O159 isolated in Japan.

abstract：:Trinitrotoluene (TNT) is an explosive chemical generally used for military, civil and industrial purposes. Therefore, TNT residue can be found in soil and ground water as an environmental pollutant. The environmental control of TNT pollution has become a critical issue because of its potential toxicity and carcinogeni...

journal_title：FEMS microbiology letters

authors： Kao CM,Wei SF,Chen SC,Yao CL,Ma C,Chien CC

更新日期：2018-01-01 00:00:00

abstract：:We have investigated the iron-dependent regulation of the psbA gene, encoding the enzyme L-ornithine N(5)-oxygenase in the rhizobacterium Pseudomonas B10. We have cloned and characterized a Pseudomonas B10 gene, designated psbS, required for psbA expression. PsbS is endowed with structural and functional features of e...

journal_title：FEMS microbiology letters

authors： Leoni L,Ambrosi C,Petrucca A,Visca P

更新日期：2002-03-05 00:00:00

abstract：:A peptide from human parotid secretion which inhibited hemagglutination of Bacteroides gingivalis 381 was purified by ultrafiltration followed by DEAE-Sephadex A-25 column chromatography and by gel filtration on Sephadex G-25, and then by reversed-phase HPLC. The complete amino acid sequence of the peptide, determined...

journal_title：FEMS microbiology letters

authors： Murakami Y,Amano A,Takagaki M,Shizukuishi S,Tsunemitsu A,Aimoto S

更新日期：1990-11-01 00:00:00

abstract：:Strains of Salmonella enteritidis expressed a novel porin with a subunit size of 35.5 kDa as shown by SDS-PAGE. This protein was expressed as a major outer membrane protein (MOMP) when grown on nutrient agar, McConkey agar or blood agar, or in Tris-succinate medium; but was only produced in trace amounts when strains ...

journal_title：FEMS microbiology letters

authors： Chart H,Frost JA,Rowe B

更新日期：1993-05-15 00:00:00

abstract：:The gacA gene of the biocontrol strain Pseudomonas fluorescens CHA0 codes for a response regulator which, together with the sensor kinase GacS (=LemA), is required for the production of exoenzymes and secondary metabolites involved in biocontrol, including hydrogen cyanide (HCN). A gacA multicopy suppressor was isolat...

journal_title：FEMS microbiology letters

authors： Blumer C,Haas D

更新日期：2000-06-01 00:00:00

abstract：:Streptomyces netropsis SD-07, the producer of novel polyene macrolide antifungal antibiotics, was isolated from soil. For the investigation of the functions of its biosynthesis genes and regulation mechanisms, a genetic operating system is necessary. In this study, we successfully transferred the plasmid DNA of pSET15...

journal_title：FEMS microbiology letters

authors： Wang XK,Jin JL

更新日期：2014-08-01 00:00:00

abstract：:Chromobacterium violaceum is a Gram-negative opportunistic human pathogen and an inhabitant of tropical soils and waterways. Although known primarily for the synthesis of the pigment violacein, and more recently as a reporter strain for quorum sensing, clinical reports of chromobacteriosis comprise the largest block o...

journal_title：FEMS microbiology letters

authors： Brumbach KC,Eason BD,Anderson LK

更新日期：2007-02-01 00:00:00

abstract：:Site-directed mutagenesis of the lamB gene was used to introduce individual cysteine substitutions at 20 sites in two regions (surface loops L7 and L8) of LamB protein significant in antibody recognition. Characterisation of cysteine mutants involved immunoblotting with three surface-specific monoclonal antibodies (mA...

journal_title：FEMS microbiology letters

authors： Notley L,Hillier C,Ferenci T

更新日期：1994-07-15 00:00:00

abstract：:Binding of meningitis-causing Escherichia coli K1 to human brain microvascular endothelial cells (HBMEC) contributes to traversal of the blood-brain barrier, which occurs in part by the mannose-sensitive binding of FimH. In this study, we showed that FimH also binds to HBMEC, independent of mannose, and identified ATP...

journal_title：FEMS microbiology letters

authors： Shin S,Kim KS

更新日期：2010-02-01 00:00:00

abstract：:Five mutations in ccpA of Bacillus megaterium with impaired functions were analysed for carbon catabolite repression. The phenotypes support the hypothesis that CcpA assumes a PurR/LacI fold. The completely inactive mutants CcpA119GE and CcpA326am cause alterations which are incompatible with that fold. A mutation wit...

journal_title：FEMS microbiology letters

authors： Kraus A,Hillen W

更新日期：1997-08-01 00:00:00

abstract：:Tolerance of the foodborne pathogen Listeria monocytogenes to sublethal concentrations of disinfectants has been frequently reported. Particularly, quaternary ammonium compounds (QACs) such as benzalkonium chloride (BC) are often used in disinfectants and also as antiseptics in food industry and hospitals. Recently, w...

journal_title：FEMS microbiology letters

authors： Müller A,Rychli K,Zaiser A,Wieser C,Wagner M,Schmitz-Esser S

更新日期：2014-12-01 00:00:00

abstract：:Competition assays measure differences between populations of bacteria after stress adaptation, populations of different bacteria and mutations in antibiotic resistance genes. We have developed a competition-based assay to evaluate if genes upregulated under starvation are important for bacterial survival. Stress resp...

journal_title：FEMS microbiology letters

authors： Havis S,Rangel J,Mali S,Bodunrin A,Housammy Z,Zimmerer R,Murphy J,Widger WR,Bark SJ

更新日期：2019-03-01 00:00:00

abstract：:We have studied the intraperoxisomal location of catalase in peroxisomes of methanol-grown Hansenula polymorpha by (immuno)cytochemical means. In completely crystalline peroxisomes, in which the crystalline matrix is composed of octameric alcohol oxidase (AO) molecules, most of the catalase protein is located in a nar...

journal_title：FEMS microbiology letters

authors： Keizer I,Roggenkamp R,Harder W,Veenhuis M

更新日期：1992-05-15 00:00:00

abstract：:We identified the minimal locus of 163-kb plasmid pSV1 of Streptomyces violaceoruber for the replication in S. lividans. This locus comprised a repA gene and an upstream 407-bp sequence containing two inverted repeats (IR-III and IR-IV) within an iteron, an AT-rich region and a 300-bp noncoding sequence (NCS). RepA pr...

journal_title：FEMS microbiology letters

authors： Chen Y,Tan H,Qin Z

更新日期：2013-12-01 00:00:00

abstract：:The purpose of this study was to develop a novel strategy for the isolation and identification of Burkholderia cepacia complex bacteria from the home environment of cystic fibrosis (CF) patients. Water and soil samples were enriched in a broth containing 0.1% l-arabinose, 0.1% l-threonine, and a mixture of selective a...

journal_title：FEMS microbiology letters

authors： Vanlaere E,Coenye T,Samyn E,Van den Plas C,Govan J,De Baets F,De Boeck K,Knoop C,Vandamme P

更新日期：2005-08-15 00:00:00

abstract：:An autonomous impala transposon trapped in Fusarium oxysporum by insertion within the niaD gene encoding nitrate reductase was introduced in the genome of the fungus Penicillium griseoroseum, a producer of pectinase enzymes. Through a phenotypic assay, we demonstrate that this element is able to excise from the niaD g...

journal_title：FEMS microbiology letters

authors： de Queiroz MV,Daboussi MJ

更新日期：2003-01-28 00:00:00

abstract：:This study reports the development and characterization of 151 sequence characterized amplified region (SCAR) markers for the seven Eimeria species that infect the domestic fowl. From this set, 84 markers are species-specific and 67 present partial specificity. The complete nucleotide sequence was derived for all mark...

journal_title：FEMS microbiology letters

authors： Fernandez S,Katsuyama AM,Kashiwabara AY,Madeira AM,Durham AM,Gruber A

更新日期：2004-09-01 00:00:00

abstract：:The primary structure of a bacteriocin produced by Enterococcus hirae DCH5 was determined by combined amino acid and DNA sequencing. Nucleotide analysis of a 2838-bp DNA fragment of E. hirae DCH5 revealed five putative ORFs. The first orf (hirJM79) encodes a 74-amino-acid peptide containing an N-terminal signal peptid...

journal_title：FEMS microbiology letters

authors： Sánchez J,Diep DB,Herranz C,Nes IF,Cintas LM,Hernández PE

更新日期：2007-05-01 00:00:00

abstract：:We have developed a novel type of a positive screen for the discovery of antibacterial compounds that target the Escherichia coli replication initiator protein DnaA. DnaA is an essential replication protein, conserved in (almost) all bacteria--including all human pathogens--and no existing antibiotics target the main ...

journal_title：FEMS microbiology letters

authors： Fossum S,De Pascale G,Weigel C,Messer W,Donadio S,Skarstad K

更新日期：2008-04-01 00:00:00

abstract：:Members of the genus Actinoplanes are considered to be representative of motile actinomycetes. To infer the flagellar diversity of Actinoplanes species, novel degenerate primers were designed for the flagellin (fliC) gene. The fliC gene of 21 Actinoplanes strains was successfully amplified and classified into two grou...

journal_title：FEMS microbiology letters

authors： Yamamura H,Hanawa K,Kusunoki M,Nakamura K,Nakagawa Y,Ishida Y,Hamada M,Otoguro M,Tamura T,Fujita N,Hayakawa M

更新日期：2011-11-01 00:00:00

abstract：:A complementary DNA of the Aspergillus nidulans chsB gene encoding chitin synthase, an essential gene for hyphal growth, was obtained by RT-PCR and expressed in Saccharomyces cerevisiae by using the GAL1 promoter in a multicopy plasmid. The biochemical characteristics of chitin synthase B (ChsB) expressed in S. cerevi...

journal_title：FEMS microbiology letters

authors： Tatsuno K,Yamada-Okabe H,Takagi M,Arisawa M,Sudoh M

更新日期：1997-04-15 00:00:00

abstract：:An 18-kDa protein that occurs in Vibrio cholerae has been described as an in vivo and low-iron regulated outer membrane antigen. Monoclonal antibodies which recognized this antigen were protective as passive vaccines in the infant rabbit model of cholera disease. In this study, those monoclonal antibodies were used in...

journal_title：FEMS microbiology letters

authors： Sciortino CV Jr

更新日期：1993-07-15 00:00:00

abstract：:E7 oncoprotein of human papillomavirus-16 (HPV-16) is constitutively produced in cervical cancer (CxCa) and is a good candidate for the design of therapeutic vaccines. In this work, the nisin-controlled expression system was used to display the E7 protein at the cell surface of the food-grade Gram-positive bacterium L...

journal_title：FEMS microbiology letters

authors： Cortes-Perez NG,Bermúdez-Humarán LG,Le Loir Y,Rodriguez-Padilla C,Gruss A,Saucedo-Cárdenas O,Langella P,Montes-de-Oca-Luna R

更新日期：2003-12-05 00:00:00

abstract：:Interspecies bacterial communication is mediated by autoinducer-2, whose synthesis depends on luxS. Due to the apparent universality of luxS (present in more than 40 bacterial species), it may have an ancient origin; however, no direct evidence is currently available. We amplified luxS in bacteria isolated from 25- to...

journal_title：FEMS microbiology letters

authors： Santiago-Rodriguez TM,Patrício AR,Rivera JI,Coradin M,Gonzalez A,Tirado G,Cano RJ,Toranzos GA

更新日期：2014-01-01 00:00:00

abstract：:Escherichia coli heat stable enterotoxin (STa) binds to isolated rat intestinal epithelial cells and triggers a cascade reaction including increase of intracellular calcium levels ([Ca(2+)](i)) and membrane bound protein kinase C (PKC) activity. In response to STa, the cytosolic PKC activity falls from 110 to 35 nmol ...

journal_title：FEMS microbiology letters

authors： Ganguly U,Chaudhury AG,Basu A,Sen PC

更新日期：2001-10-16 00:00:00

abstract：:One of the commercial strains of Flammulina velutipes was analyzed for the presence of double-stranded RNA (dsRNA) elements to examine the underlying mechanism of strain degeneration. As a result, two dsRNA elements sized 1.9 and 1.8 kb were detected in mycelium derived from spontaneously brown-colored fruit body. The...

journal_title：FEMS microbiology letters

authors： Magae Y,Hayashi N

更新日期：1999-11-15 00:00:00

abstract：:The signal that triggers the expression of the ace operon and, in turn, the transition of central metabolism's architecture from acetogenic to gluconeogenic in Escherichia coli remains elusive despite extensive research both in vivo and in vitro. Here, with the aid of flux analysis together with measurements of the en...

journal_title：FEMS microbiology letters

authors： El-Mansi M,Phue JN,Shiloach J

更新日期：2021-01-08 00:00:00

abstract：:When Escherichia coli STII was applied to the serosa of the ileum at a concentration of 40 micrograms/ml, an acceleration of the spontaneous motility and a weak contraction were induced 2-3 min later. The induction was not affected by the addition of atropine (10(-6) M), but was abolished by the addition of papaverine...

journal_title：FEMS microbiology letters

authors： Hitotsubashi S,Akagi M,Saitou A,Yamanaka H,Fujii Y,Okamoto K

更新日期：1992-01-15 00:00:00

abstract：:The aim of this study was to evaluate the ability of a nested PCR system to detect Salmonella senftenberg in raw oysters. The specific primers of the PCR were derived from the invA gene sequence, essential for Salmonella invasiveness into epithelial cells. First, for the extraction of DNA, four methods (guanidine isot...

journal_title：FEMS microbiology letters

authors： Vázquez-Novelle MD,Pazos AJ,Abad M,Sánchez JL,Pérez-Parallé ML

更新日期：2005-02-01 00:00:00

abstract：:The enzymatic activity of salivary amylase bound to the surface of several species of oral streptococci was determined by the production of acid from starch and by the degradation of maltotetraose to glucose in a coupled, spectrophotometric assay. Most strains able to bind amylase exhibited functional enzyme on their ...

journal_title：FEMS microbiology letters

authors： Douglas CW,Heath J,Gwynn JP

更新日期：1992-04-15 00:00:00