Abstract:
:The role in virulence of Actinobacillus pleuropneumoniae urease activity was investigated. A urease-negative mutant was isolated following transposon mutagenesis with a mini-Tn10 derivative. Both the parent strain and the urease-negative mutant exhibited identical LD50 values in a murine infection model. Pig challenge confirmed that the urease-negative mutant was fully virulent, since experimental inoculation with 5 x 10(7) colony forming units resulted in an acute disease indistinguishable from that produced by the wild-type strain at the same dose. Our results demonstrate that urease activity is not required for the development of acute pleuropneumonia.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Tascón Cabrero RI,Vázquez-Boland JA,Gutiérrez CB,Rodríguez-Barbosa JI,Rodríguez-Ferri EFdoi
10.1111/j.1574-6968.1997.tb10266.xsubject
Has Abstractpub_date
1997-03-01 00:00:00pages
53-7issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(97)00012-8journal_volume
148pub_type
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