A xylanase produced by the rumen anaerobic protozoan Polyplastron multivesiculatum shows close sequence similarity to family 11 xylanases from gram-positive bacteria.

abstract：:The genes encoding transferrin-binding proteins (TBPs) 1 and 2 of Neisseria meningitidis and N. gonorrhoeae were used as model loci in a novel method of cloning (twin N-terminal polymerase chain reaction; TNT-PCR) involving amplification between the 5' ends of two genes. Primers were based on N-terminal amino-acid seq...

journal_title：FEMS microbiology letters

authors： Wilton J,Ala'Aldeen D,Palmer HM,Borriello SP

更新日期：1993-02-15 00:00:00

abstract：:The glycolytic pathway of the hyperthermophilic archaea that belong to the order Thermococcales (Pyrococcus, Thermococcus and Palaeococcus) differs significantly from the canonical Embden-Meyerhof pathway in bacteria and eukarya. This archaeal glycolysis variant consists of several novel enzymes, some of which catalyz...

journal_title：FEMS microbiology letters

authors： van de Werken HJ,Verhees CH,Akerboom J,de Vos WM,van der Oost J

更新日期：2006-07-01 00:00:00

abstract：:Antimicrobial resistance (AMR) has emerged as one of the most pressing threats to public health. AMR evolution occurs in the clinic but also in the environment, where antibiotics and heavy metals can select and co-select for AMR. While the selective potential of both antibiotics and metals is increasingly well-charact...

journal_title：FEMS microbiology letters

authors： Vos M,Sibleyras L,Lo LK,Hesse E,Gaze W,Klümper U

更新日期：2020-02-01 00:00:00

abstract：:The purpose of this study was to develop a novel strategy for the isolation and identification of Burkholderia cepacia complex bacteria from the home environment of cystic fibrosis (CF) patients. Water and soil samples were enriched in a broth containing 0.1% l-arabinose, 0.1% l-threonine, and a mixture of selective a...

journal_title：FEMS microbiology letters

authors： Vanlaere E,Coenye T,Samyn E,Van den Plas C,Govan J,De Baets F,De Boeck K,Knoop C,Vandamme P

更新日期：2005-08-15 00:00:00

abstract：:CRISPR-Cas systems provide adaptive immunity against mobile genetic elements, but employment of this resistance mechanism is often reported with a fitness cost for the host. Whether or not CRISPR-Cas systems are important barriers for the horizontal spread of conjugative plasmids, which play a crucial role in the spre...

journal_title：FEMS microbiology letters

authors： van Sluijs L,van Houte S,van der Oost J,Brouns SJ,Buckling A,Westra ER

更新日期：2019-03-01 00:00:00

abstract：:The expression of the rbp genes, which encode RNA-binding proteins with a single RNA-recognition motif and a glycine-rich sequence, is known to increase at low temperature in cyanobacteria. We previously showed that their regulation involved both transcription and mRNA stability. In the present study, various reporter...

journal_title：FEMS microbiology letters

authors： Ehira S,Ohmori M,Sato N

更新日期：2005-10-01 00:00:00

abstract：:Herbaspirillum seropedicae strains mutated in the nifX or orf1 genes showed 90% or 50% reduction in nitrogenase activity under low levels of iron or molybdenum respectively. Mutations in nifX or orf1 genes did not affect nif gene expression since a nifH::lacZ fusion was fully active in both mutants. nifX and the conti...

journal_title：FEMS microbiology letters

authors： Klassen G,de Oliveira Pedrosa F,de Souza EM,Yates MG,Rigo LU

更新日期：2003-07-29 00:00:00

abstract：:Paracoccus pantotrophus grown anaerobically under denitrifying conditions expressed similar levels of the periplasmic nitrate reductase (NAP) when cultured in molybdate- or tungstate-containing media. A native PAGE gel stained for nitrate reductase activity revealed that only NapA from molybdate-grown cells displayed ...

journal_title：FEMS microbiology letters

authors： Gates AJ,Hughes RO,Sharp SR,Millington PD,Nilavongse A,Cole JA,Leach ER,Jepson B,Richardson DJ,Butler CS

更新日期：2003-03-28 00:00:00

abstract：:Dextranase specifically hydrolyzes dextran and is used to produce functional isomalto-saccharide prebiotics. Moreover, dextranase is used as an additive in mouthwash to remove dental plaque. We cloned and expressed the dextranase gene of the marine bacterium Bacillus aquimaris S5. The length of the BaDex gene was 1788...

journal_title：FEMS microbiology letters

authors： Dong D,Wang X,Deng T,Ning Z,Tian X,Zu H,Ding Y,Wang C,Wang S,Lyu M

更新日期：2021-01-20 00:00:00

abstract：:In microbial corrinoid-dependent methyltransferase systems, adventitious Co(I)-corrinoid oxidation halts catalysis and necessitates repair by ATP-dependent reductive activases. RamA, an activase with a C-terminal ferredoxin domain with two [4Fe-4S] clusters from methanogenic archaea, has been far less studied than the...

journal_title：FEMS microbiology letters

authors： Huening KA,Jiang R,Krzycki JA

更新日期：2020-09-01 00:00:00

abstract：:A novel cry1A was cloned from Bacillus thuringiensis strain BT8 and expressed in the B. thuringiensis acrystalliferous mutant HD73(-). The gene, designated cry1Ah1, encoded a protein with a molecular weight of 134 kDa. Reverse transcriptase-PCR and Western blotting showed that Cry1Ah was expressed in the host strain B...

journal_title：FEMS microbiology letters

authors： Xue J,Liang G,Crickmore N,Li H,He K,Song F,Feng X,Huang D,Zhang J

更新日期：2008-03-01 00:00:00

abstract：:We have determined the genetic basis of minocycline resistance in a strain of Streptococcus infantis isolated from a healthy human oral cavity. We demonstrate that tet(S), identical to tet(S) found on the enterococcal conjugative transposon Tn6000, is responsible for the observed resistance. The gene is located on a s...

journal_title：FEMS microbiology letters

authors： Ciric L,Brouwer MS,Mullany P,Roberts AP

更新日期：2014-04-01 00:00:00

abstract：:The DnaK chaperone system is involved in various cellular processes such as the control of the folded and oligomeric state of proteins under stress and non-stress conditions. In this study we functionally characterised the homologues of the DnaK system from Clostridium acetobutylicum DnaK, DnaJ, GrpE and OrfA were het...

journal_title：FEMS microbiology letters

authors： Rüngeling E,Laufen T,Bahl H

更新日期：1999-01-01 00:00:00

abstract：:Lactobacilli occupy specific ecological niches, where they represent a major component of foods, human and animal microbial communities. Employing these bacteria in industrial fermentations or for human health benefits exposes them to certain life-threatening conditions where their ability to adapt plays a key role in...

journal_title：FEMS microbiology letters

authors： Hussain MA,Hosseini Nezhad M,Sheng Y,Amoafo O

更新日期：2013-12-01 00:00:00

abstract：:The enterotoxigenic Escherichia coli (ETEC) strain Ec2173, causing post weaning diarrhoea in swine, harbours six plasmids ranging from 13 to 200 kb in size. The heat stable toxin genes sta, stb and a tetracycline resistance gene were located on a self conjugative 120-kb plasmid, called pTC. In the cloned ColE1 type or...

journal_title：FEMS microbiology letters

authors： Olasz F,Fekete PZ,Blum-Oehler G,Boldogkoi Z,Nagy B

更新日期：2005-03-15 00:00:00

abstract：:Cytoplasmic precursors of the peptidoglycan biosynthetic pathway were purified from vancomycin-treated, glycopeptide-sensitive and -resistant strains of Enterococcus faecium. Resistance was due to production of a modified precursor, UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-lactate, where lactate was identified on the basi...

journal_title：FEMS microbiology letters

authors： Messer J,Reynolds PE

更新日期：1992-07-01 00:00:00

abstract：:The glycoside hydrolase (GH) 16 family of Coprinopsis cinerea includes 15 members distributed in four subgroups (A1, A2, B and D) by phylogenetic analysis. The expression patterns match well with the requirement of wall-softening in the germination of basidiospores, hyphal growth and branching, primordium formation, s...

journal_title：FEMS microbiology letters

authors： Kang L,Zhu Y,Bai Y,Yuan S

更新日期：2019-04-01 00:00:00

abstract：:This commentary describes an assessment exercise known as the TRIPSE (Tri-Partite Problem Solving Exercise) that mimics science in operation. Students frame hypotheses based on limited data, design experiments to test them, which they later revise with new information. It is emphasised that there are no single correct...

journal_title：FEMS microbiology letters

authors： Nastos S,Rangachari PK

更新日期：2016-08-01 00:00:00

abstract：:Legionella pneumophila is known as a facultative intracellular parasite of free-living soil and freshwater amoebae, of which several species have been shown to support the growth of the pathogenic bacteria. We report for the first time the behaviour of two strains (c2c and Z503) of the amoeba Willaertia magna towards ...

journal_title：FEMS microbiology letters

authors： Dey R,Bodennec J,Mameri MO,Pernin P

更新日期：2009-01-01 00:00:00

abstract：:The plant-growth promoting rhizobacterium Azospirillum lipoferum strain 4B generates in vitro a stable phase variant designated 4VI at frequencies of 10(-4) to 10(-3) per cell per generation. Variant 4VI displays pleitropic modifications, such as the loss of swimming motility and the inability to assimilate certain su...

journal_title：FEMS microbiology letters

authors： Vial L,Pothier JF,Normand P,Moënne-Loccoz Y,Bally R,Wisniewski-Dyé F

更新日期：2004-07-15 00:00:00

abstract：:Methods for in vivo monitoring of redox changes in different cellular compartments have been developed in recent years, and are mostly based on redox-sensitive variants of the green fluorescent protein (GFP). However, due to the thermodynamic stability of the introduced reactive disulfide bond, these sensors are limit...

journal_title：FEMS microbiology letters

authors： Delic M,Mattanovich D,Gasser B

更新日期：2010-05-01 00:00:00

abstract：:An investigation of mortality of piglets through clinical signs, post-mortem, histopathology and bacteriological analyses revealed the causal organism to be Escherichia coli, mainly O149:K91:K88 which belongs to the enterotoxigenic biotypes. Molecular characterization and epidemiologic analysis elucidated it as shiga-...

journal_title：FEMS microbiology letters

authors： Kanengoni AT,Thomas R,Gelaw AK,Madoroba E

更新日期：2017-02-01 00:00:00

abstract：:Agrobacterium tumefaciens is the causal agent of crown gall disease and is a vector for DNA transfer in transgenic plants. The transformation process by A. tumefaciens has been widely studied, but the attachment stage has not been well characterized. Most measurements of attachment have used microscopy and colony coun...

journal_title：FEMS microbiology letters

authors： Petrovicheva A,Joyner J,Muth TR

更新日期：2017-10-02 00:00:00

abstract：:We present evidence that glycine betaine (betaine) which was synthesized from choline was excreted and reaccumulated in osmoregulating cells of Escherichia coli. Choline which was accumulated in bet mutants defective in betaine synthesis was shown to be excreted in response to betaine uptake. Our data suggest that E. ...

journal_title：FEMS microbiology letters

authors： Lamark T,Styrvold OB,Strøm AR

更新日期：1992-09-15 00:00:00

abstract：:Mini-Tn10luxABcam/Ptac-ATS was constructed in order to develop a luciferase-transducing bacteriophage for detecting Escherichia coli O157:H7. The transposon was designed to deliver a 3.6-kb insertion that confers n-decanal-dependent bioluminescence and resistance to chloramphenicol and was constructed using mini-Tn10c...

journal_title：FEMS microbiology letters

authors： Waddell TE,Poppe C

更新日期：2000-01-15 00:00:00

abstract：:The anr gene of Pseudomonas aeruginosa encodes a transcriptional regulator of anaerobic gene expression, homologous to the Fnr protein of Escherichia coli. We report here that Anr has a role in regulating the activity of the aerobic respiratory chain of P. aeruginosa. Strains with internal deletions in their anr gene ...

journal_title：FEMS microbiology letters

authors： Ray A,Williams HD

更新日期：1997-11-15 00:00:00

abstract：:The major haem component in the black pigment of Porphyromonas gingivalis is the mu-oxo bishaem of iron protoporphyrin IX and formation and cell-surface binding of this haem species is proposed as an extracellular buffer against reactive oxidants [Smalley, J.W. et al. (1998) Biochem. J. 331, 681-685]. P. gingivalis ce...

journal_title：FEMS microbiology letters

authors： Smalley JW,Birss AJ,Silver J

更新日期：2000-02-01 00:00:00

abstract：:A structural characterization of polysaccharides extracted from the aposymbiotically cultured photobiont of the lichen Ramalina gracilis was carried out in order to compare them with those previously found in the symbiotic thallus. The photobiont was isolated from thallus fragments, following the method of Yamamoto, a...

journal_title：FEMS microbiology letters

authors： Cordeiro LM,Carbonero ER,Sassaki GL,Reis RA,Stocker-Wörgötter E,Gorin PA,Iacomini M

更新日期：2005-03-01 00:00:00

abstract：:The central carbon metabolism is well investigated in bacteria, but this is not the case for archaea. MJ0400-His(6) from Methanocaldococcus jannaschii catalyzes the cleavage of fructose-1,6-bisphosphate (FBP) to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate with a V(max) of 33 mU mg(-1) and a K(m) of 430 m...

journal_title：FEMS microbiology letters

authors： Samland AK,Wang M,Sprenger GA

更新日期：2008-04-01 00:00:00

abstract：:The chimeric plasmid pMY10 containing the origin of replication of pAL5000, the origin of replication of pBR322, the origin of transfer of pRK2 and a kanamycin resistance gene was constructed and successfully transferred by conjugation from Escherichia coli harbouring the helper plasmid pRK4.24 into Mycobacterium smeg...

journal_title：FEMS microbiology letters

authors： Lazraq R,Clavel-Sérès S,David HL,Roulland-Dussoix D

更新日期：1990-05-01 00:00:00