Ca2+ stabilization of respiratory complex I from Escherichia coli.

Abstract:

:Stability of the membrane-bound and purified H+-translocating NADH:ubiquinone oxidoreductase, Complex I, was studied. The loss of the enzyme activity is strongly increased by alkaline pH and dilution of the sample. Complex I inactivation is prevented specifically by a low concentration of Ca2+ and/or an intracellular stabilization factor (ISF). The action of both, Ca2+ and ISF, on Complex I stability is interdependent. The data are discussed in terms of a release of structural Ca2+ as a reason for Complex I decay and an effect of ISF on the affinity and/or accessibility of Ca2+-binding site.

journal_name

FEMS Microbiol Lett

authors

Belevich G,Belevich N,Knuuti J,Verkhovskaya M

doi

10.1093/femsle/fny097

subject

Has Abstract

pub_date

2018-06-01 00:00:00

issue

12

eissn

0378-1097

issn

1574-6968

pii

4969679

journal_volume

365

pub_type

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