A genome map of Salmonella enterica serovar Agona: numerous insertions and deletions reflecting the evolutionary history of a human pathogen.

abstract：:Helicobacter pullorum and Campylobacter lari are rarely isolated from humans with acute enteritis. Hitherto the two species could only be identified by genotypic techniques. Gas liquid chromatography of whole cell fatty acid extracts is described as the first phenotypic method for discrimination of the two species. Ch...

journal_title：FEMS microbiology letters

authors： Steinbrueckner B,Haerter G,Pelz K,Burnens A,Kist M

更新日期：1998-11-15 00:00:00

abstract：:Corynebacterium diphtheriae strains express non-fimbrial surface proteins able to recognize and bind to specific host cells receptors. Protein extracts were obtained from bacterial cells by mechanical process and ammonium sulfate precipitation at 25 and 45% (w/v) saturation. SDS-PAGE analysis of the extracts detected ...

journal_title：FEMS microbiology letters

authors： Colombo AV,Hirata R Jr,de Souza CM,Monteiro-Leal LH,Previato JO,Formiga LC,Andrade AF,Mattos-Guaraldi AL

更新日期：2001-04-13 00:00:00

abstract：:Outer membrane-associated proteins of 18 kDa were expressed by enteroaggregative Escherichia coli (EAggEC) belonging to serotypes O126:H27 and O44:H18, which hybridized with a probe derived from a plasmid necessary for enteroaggregative adhesion. The 18 kDa proteins expressed by strains of E. coli, belonging to these ...

journal_title：FEMS microbiology letters

authors： Chart H,Smith HR,Rowe B

更新日期：1995-10-01 00:00:00

abstract：:The natural life cycle of Vibrio cholerae involves the transitioning of cells between different environmental surfaces such as the chitinous shell of Crustaceae and the epithelial layer of the human intestine. Previous studies using static biofilm systems showed a strict dependence of biofilm formation on the vps and ...

journal_title：FEMS microbiology letters

authors： Müller J,Miller MC,Nielsen AT,Schoolnik GK,Spormann AM

更新日期：2007-10-01 00:00:00

abstract：:Site-directed mutagenesis of the lamB gene was used to introduce individual cysteine substitutions at 20 sites in two regions (surface loops L7 and L8) of LamB protein significant in antibody recognition. Characterisation of cysteine mutants involved immunoblotting with three surface-specific monoclonal antibodies (mA...

journal_title：FEMS microbiology letters

authors： Notley L,Hillier C,Ferenci T

更新日期：1994-07-15 00:00:00

abstract：:In order to investigate whether the iron acquisition mechanisms of Staphylococcus aureus are induced by iron restriction in vitro, we examined S. aureus ATCC 6538 for production of siderophore and expression of transferrin-binding protein (SA-tbp) in normal or deferrated brain heart infusion broth (BHI). Siderophore p...

journal_title：FEMS microbiology letters

authors： Lim Y,Shin SH,Lee SI,Kim IS,Rhee JH

更新日期：1998-06-01 00:00:00

abstract：:We characterized Yersinia enterocolitica and Yersinia pseudotuberculosis insertion sequences related to insertion sequence 1541, recently identified in Yersinia pestis. For each of the two species, two insertion sequence copies were cloned and sequenced. Genetic elements from Y. pseudotuberculosis were almost identica...

journal_title：FEMS microbiology letters

authors： Devalckenaere A,Odaert M,Trieu-Cuot P,Simonet M

更新日期：1999-07-01 00:00:00

abstract：:Picrophilus torridus has been shown to degrade glucose via a nonphosphorylative Entner-Doudoroff (ED) pathway. Here we report the characterization of a key enzyme of this pathway, glycerate kinase (2-phosphoglycerate forming). The enzyme was purified 5,100-fold to homogeneity. The 95 kDa homodimeric protein catalyzed ...

journal_title：FEMS microbiology letters

authors： Reher M,Bott M,Schönheit P

更新日期：2006-06-01 00:00:00

abstract：:Molecular information suggests that there is a broad diversity of acetogens in the rumen, distinct from any currently isolated acetogens. We combined molecular analysis with enrichment culture techniques to investigate this diversity further. Methane-inhibited, hydrogenotrophic enrichment cultures produced acetate as ...

journal_title：FEMS microbiology letters

authors： Gagen EJ,Padmanabha J,Denman SE,McSweeney CS

更新日期：2015-07-01 00:00:00

abstract：:Bacillus subtilis cells must have cytochromes for growth and can synthesize cytochromes of a-, b-, c-, d-, and o-types. After a long lag, our knowledge of the structure, genetics and specific role for these cytochromes is now growing exponentially as the result of recent research. This progress is reviewed here and in...

journal_title：FEMS microbiology letters

authors： von Wachenfeldt C,Hederstedt L

更新日期：1992-12-15 00:00:00

abstract：:The presence of insertion sequence IS1 in 70 multiple-antibiotic resistant clinical strains was determined. This 70-strain collection comprised 46 Escherichia coli, 18 Salmonella and 6 Shigella strains. The presence of IS1 was detected in the chromosome and plasmids of 73% and 63% of the strains, respectively, and 51%...

journal_title：FEMS microbiology letters

authors： Ramirez-Santos J,Alvarez G,Cisneros E,Gomez-Eichelmann MC

更新日期：1992-06-01 00:00:00

abstract：:Attachment of soil bacteria to plant cells is supposedly the very early step required in plant-microbe interactions. Attachment also is an initial step for the formation of microbial biofilms on plant roots. For the rhizobia-legume symbiosis, various mechanisms and diverse surface molecules of both partners have been ...

journal_title：FEMS microbiology letters

authors： Rodríguez-Navarro DN,Dardanelli MS,Ruíz-Saínz JE

更新日期：2007-07-01 00:00:00

abstract：:The presence of diatoms in victim's internal organs has been regarded as a gold biological evidence of drowning. The idea becomes true at the advent of DNA metabarcoding. Unfortunately, the DNA barcode of diatoms are far from being applicable due to neither consensus on the barcode and nor reliable reference library.I...

journal_title：FEMS microbiology letters

authors： Liu M,Zhao Y,Sun Y,Wu P,Zhou S,Ren L

更新日期：2020-09-01 00:00:00

abstract：:A structural characterization of polysaccharides extracted from the aposymbiotically cultured photobiont of the lichen Ramalina gracilis was carried out in order to compare them with those previously found in the symbiotic thallus. The photobiont was isolated from thallus fragments, following the method of Yamamoto, a...

journal_title：FEMS microbiology letters

authors： Cordeiro LM,Carbonero ER,Sassaki GL,Reis RA,Stocker-Wörgötter E,Gorin PA,Iacomini M

更新日期：2005-03-01 00:00:00

abstract：:Competition assays measure differences between populations of bacteria after stress adaptation, populations of different bacteria and mutations in antibiotic resistance genes. We have developed a competition-based assay to evaluate if genes upregulated under starvation are important for bacterial survival. Stress resp...

journal_title：FEMS microbiology letters

authors： Havis S,Rangel J,Mali S,Bodunrin A,Housammy Z,Zimmerer R,Murphy J,Widger WR,Bark SJ

更新日期：2019-03-01 00:00:00

abstract：:Contrary to its effect on rich medium, D-cycloserine showed no bactericidal effect on Zymomonas mobilis cells cultured on mineral medium. Addition of a mixture of glycine and glutamic acid to the mineral medium restored its bactericidal action. However, mutant enrichments run in these conditions were biased, with most...

journal_title：FEMS microbiology letters

authors： Pencreac'h G,Aït-Abdelkader N,Joset F,Baratti JC

更新日期：1996-09-01 00:00:00

abstract：:We have isolated the abc1 gene from the fission yeast Schizosaccharomyces pombe. Sequence analysis suggests that the Abc1 protein is a member of the ABC superfamily of transporters and is composed of two structurally homologous halves, each consisting of a hydrophobic region of six transmembrane domains and a hydrophi...

journal_title：FEMS microbiology letters

authors： Christensen PU,Davis K,Nielsen O,Davey J

更新日期：1997-02-01 00:00:00

abstract：:The beta-toxin gene from Clostridium perfringens type C was cloned and expressed as a glutathione S-transferase fusion protein in Escherichia coli. The DNA sequence was determined and compared to the type B sequence. Two nucleotide differences were found in the protein coding sequence, resulting in one amino acid diff...

journal_title：FEMS microbiology letters

authors： Steinthorsdottir V,Fridriksdottir V,Gunnarsson E,Andrésson OS

更新日期：1995-08-01 00:00:00

abstract：:Lipoic acid is an essential cofactor for a variety of mitochondrial enzymes. We have characterised a gene from Saccharomyces cerevisiae which appears to encode a protein involved in the attachment of lipoic acid groups to the pyruvate dehydrogenase and glycine decarboxylase complexes. The predicted protein product of ...

journal_title：FEMS microbiology letters

authors： Marvin ME,Williams PH,Cashmore AM

更新日期：2001-05-15 00:00:00

abstract：:The restriction deficient mutant 8195 of Acholeplasma laidlawii strain JA1 was transformed by the promiscuous streptococcal plasmid vector pNZ18 at a frequency of 4 x 10(-4)/cfu. The plasmid was maintained without structural rearrangements but was lost in the absence of a selection pressure, i.e. kanamycin or neomycin...

journal_title：FEMS microbiology letters

authors： Sundström TK,Wieslander A

更新日期：1990-10-01 00:00:00

abstract：:The aim of this study was the production of the homopolyester poly(4-hydroxybutyric acid) (poly(4HB)) with recombinant strains of Escherichia coli. Wild-type strains and other widely used non-recombinant strains of E. coli are not able to produce polyhydroxyalkanoic acids (PHA) as storage compounds and cannot utilize ...

journal_title：FEMS microbiology letters

authors： Hein S,Söhling B,Gottschalk G,Steinbüchel A

更新日期：1997-08-15 00:00:00

abstract：:Clostridium difficile is the causative agent for pseudomembranous colitis in humans. Toxic strains of C. difficile produce two toxins, toxin A and toxin B. A reliable and definitive method of typing the toxic strains of C. difficile is needed since nosocomial cross infection is a primary concern in hospitals and other...

journal_title：FEMS microbiology letters

authors： McMillin DE,Muldrow LL

更新日期：1992-04-01 00:00:00

abstract：:The lipoate acetyltransferase (E2p) subunits of the pyruvate dehydrogenase (PDH) complex of Escherichia coli have three tandemly repeated lipoyl domains, although net deletions of one or two has no apparent effect on the activity of the purified complexes. Plasmids containing IPTG-inducible aceEF-lpd operons, which en...

journal_title：FEMS microbiology letters

authors： Machado RS,Clark DP,Guest JR

更新日期：1992-12-15 00:00:00

abstract：:The genus Pycnoporus forms a group of four species known especially for producing high redox potential laccases suitable for white biotechnology. A sample of 36 Pycnoporus strains originating from different geographical areas was studied to seek informative molecular markers for the typing of new strains in laboratory...

journal_title：FEMS microbiology letters

authors： Lesage-Meessen L,Haon M,Uzan E,Levasseur A,Piumi F,Navarro D,Taussac S,Favel A,Lomascolo A

更新日期：2011-12-01 00:00:00

abstract：:A synthetic version of the ligA gene encoding the NAD-dependent DNA ligase from the Arctic bacterium Pseudoalteromonas haloplanktis was substituted for its homolog in the chromosome of Mycobacterium smegmatis. The resulting recombinant strain grew identically to the parent strain at permissive temperatures but failed ...

journal_title：FEMS microbiology letters

authors： Pinto CT,Nano FE

更新日期：2015-09-01 00:00:00

abstract：:The amino acid composition and structure of the peptidoglycan from Heliobacillus mobilis was determined by one- and two-dimensional thin-layer chromatography of completely and partially hydrolysed cell wall preparations. The structure was found to be of the A3 gamma type, with L,L-diaminopimelate in position 3, D-alan...

journal_title：FEMS microbiology letters

authors： Pickett MW,Weiss N,Kelly DJ

更新日期：1994-09-15 00:00:00

abstract：:Bradyrhizobium japonicum has two types of flagella. One has thin filaments consisting of the 33-kDa flagellins FliCI and FliCII (FliCI-II) and the other has thick filaments consisting of the 65-kDa flagellins FliC1, FliC2, FliC3, and FliC4 (FliC1-4). To investigate the roles of each flagellum in competition for nodula...

journal_title：FEMS microbiology letters

authors： Althabegoiti MJ,Covelli JM,Pérez-Giménez J,Quelas JI,Mongiardini EJ,López MF,López-García SL,Lodeiro AR

更新日期：2011-06-01 00:00:00

abstract：:Loop-mediated isothermal amplification (LAMP) assay detected Salmonella within 60 min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella. The sensitivity of the LAMP assay w...

journal_title：FEMS microbiology letters

authors： Hara-Kudo Y,Yoshino M,Kojima T,Ikedo M

更新日期：2005-12-01 00:00:00

abstract：:The lipid modification and processing of truncated mutants of the colicin A lysis protein were observed after overproduction by Escherichia coli bacteria, but at a rate far slower than that of the wild-type. The unmodified precursor form of the mutants was stable over hour(s). The truncated mutants provoked lethality,...

journal_title：FEMS microbiology letters

authors： Cavard D

更新日期：1994-04-01 00:00:00

abstract：:Two L-methionine-enriched mutants, SN-78 and SE-57, were isolated in a sulphur-deficient medium from the methylotrophic yeast Candida boidinii ICCF26. No significant differences were detected between the L-cysteine pools of the mutants and the wild-type. In mutant strain SE-57, S-adenosylmethionine and ergosterol leve...

journal_title：FEMS microbiology letters

authors： Avram D,Stan R

更新日期：1992-11-01 00:00:00