Characterization of a novel vip3-type gene from Bacillus thuringiensis and evidence of its presence on a large plasmid.

Abstract:

:A novel vip3-type gene named vip3LB has been isolated from Bacillus thuringiensis strain BUPM95. The corresponding secreted vegetative insecticidal protein was active against the lepidopteran insect Ephestia kuehniella. The vip3LB gene was shown, for the first time, to be carried by the large plasmid containing the cry1Ia genes of B. thuringiensis. The nucleotide sequence predicted a protein of 789 amino acids residues with a calculated molecular mass of 88.5kDa. Both nucleotide and amino acid sequences similarity analysis revealed that vip3LB is a new vip3-type gene, presenting several differences with the other vip3-type genes. Heterologous expression of the vip3LB under the control of the strong promoter P(BAD) was performed in Escherichia coli and the produced protein conferred insecticidal activity against Ephestia kuehniella. This novel vegetative insecticidal protein Vip3LB could be a very useful biological control agent.

journal_name

FEMS Microbiol Lett

authors

Mesrati LA,Tounsi S,Jaoua S

doi

10.1016/j.femsle.2005.02.007

keywords:

subject

Has Abstract

pub_date

2005-03-15 00:00:00

pages

353-8

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(05)00090-X

journal_volume

244

pub_type

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