Abstract:
:pSA1.1 is a 9.1-kb multicopy plasmid originally isolated from Streptomyces cyaneus (formerly S. azureus) ATCC 14921. This plasmid accumulates single-stranded DNA in S. lividans and is therefore considered to replicate by a rolling-circle replication. In the present work, the rep gene encoding the replication initiator protein and the replication origin ori of pSA1.1 were determined. The rep and ori are located on separate regions. The Rep protein of pSA1.1 belongs to superfamily I which includes A proteins of phages. Nucleotide sequence of the surrounding putative nicking site of pSA1.1 shows good agreement with those of the pC194 group plasmids and phages. The direction of replication was also determined.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Yokoyama E,Matsuzaki Y,Doi K,Ogata Sdoi
10.1111/j.1574-6968.1998.tb13305.xsubject
Has Abstractpub_date
1998-12-01 00:00:00pages
103-9issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(98)00469-8journal_volume
169pub_type
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