An improved aminoglycoside resistance gene cassette for use in gram-negative bacteria and Streptomyces.


:A cloning cassette, carrying a modified amino-glycoside resistance gene (neo) from transposon Tn5 was constructed. Three restriction sites internal to the neo gene were eliminated by in vitro mutagenesis, allowing their use in designing new cloning vectors. The original, suboptimal transcription promoter was replaced with a synthetic sequence corresponding to the consensus for E. coli and Streptomyces promoters. The cassette has numerous restriction sites for easy subcloning of the promoter, the coding sequence or the whole gene.


FEMS Microbiol Lett


Denis F,Brzezinski R





Has Abstract


1991-07-01 00:00:00












  • Induction of baroresistance by hydrogen peroxide, ethanol and cold-shock in Saccharomyces cerevisiae.

    abstract::The acquisition of tolerance to high hydrostatic pressure of 220 MPa (HHP) in response to a 0.4 mM hydrogen peroxide, 6% ethanol and cold-shock (10 degrees C) pretreatment for different lengths of times was studied in the yeast Saccharomyces cerevisiae. The protection conferred by these different treatments was simila...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Palhano FL,Orlando MT,Fernandes PM

    更新日期:2004-04-01 00:00:00

  • Anaerobic degradation of protocatechuate (3,4-dihydroxybenzoate) by Thauera aromatica strain AR-1.

    abstract::The denitrifying bacterium Thauera aromatica strain AR-1 grows anaerobically with protocatechuate (3,4-dihydroxybenzoate (DHB)) as sole energy and carbon source. This bacterium harbors two distinct pathways for degradation of aromatic compounds, the benzoyl-coenzyme A (CoA) pathway for benzoate degradation and the hyd...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Philipp B,Kemmler D,Hellstern J,Gorny N,Caballero A,Schink B

    更新日期:2002-06-18 00:00:00

  • Enhancement and repression of cyanide-insensitive respiration in Aspergillus niger.

    abstract::The biosynthesis of the alternative oxidase protein consisting of cyanide-insensitive and salicylhydroxamic acid-sensitive respiration in Aspergillus niger was investigated. Cyanide-insensitive respiration was enhanced by addition of antimycin A to the incubation mixture, but repressed by the addition of cycloheximide...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kirimura K,Matsui T,Sugano S,Usami S

    更新日期:1996-08-01 00:00:00

  • Isolation of the dxr gene of Zymomonas mobilis and characterization of the 1-deoxy-D-xylulose 5-phosphate reductoisomerase.

    abstract::The gene encoding the second enzyme of the 2C-methyl-D-erythritol 4-phosphate (MEP) pathway for isopentenyl diphosphate biosynthesis, 1-deoxy-D-xylulose 5-phosphate (DXP) reductoisomerase, was cloned and sequenced from Zymomonas mobilis. The deduced amino acid sequence showed the highest identity (48.2%) to the DXP re...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Grolle S,Bringer-Meyer S,Sahm H

    更新日期:2000-10-01 00:00:00

  • Disruption of response regulator gene, devR, leads to attenuation in virulence of Mycobacterium tuberculosis.

    abstract::The devR-devS two-component system of Mycobacterium tuberculosis was identified earlier and partially characterized in our laboratory. A devR::kan mutant of M. tuberculosis was constructed by allelic exchange. The devR mutant strain showed reduced cell-to-cell adherence in comparison to the parental strain in laborato...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Malhotra V,Sharma D,Ramanathan VD,Shakila H,Saini DK,Chakravorty S,Das TK,Li Q,Silver RF,Narayanan PR,Tyagi JS

    更新日期:2004-02-16 00:00:00

  • A PCR fingerprinting technique to distinguish isolates of Lactococcus lactis.

    abstract::A fingerprinting technique similar to repetitive extragenic palindromic PCR was developed to identify strains of Lactococcus lactis. The method distinguishes closely related strains and discriminates among some with identical ldh sequences. The fingerprinting primer LL-Rep1 complements a moderately repeated sequence f...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Urbach E,Schindler C,Giovannoni SJ

    更新日期:1998-05-01 00:00:00

  • PCR-based detection of composite transposons and translocatable units from oral metagenomic DNA.

    abstract::A composite transposon is a mobile genetic element consisting of two insertion sequences (ISs) flanking a segment of cargo DNA often containing antibiotic resistance (AR) genes. Composite transposons can move as a discreet unit. There have been recently several reports on a novel mechanism of movement of an IS26-based...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Tansirichaiya S,Mullany P,Roberts AP

    更新日期:2016-09-01 00:00:00

  • Fluorescence in situ hybridisation (FISH)--the next generation.

    abstract::Fluorescence in situ hybridisation (FISH) has become one of the major techniques in environmental microbiology. The original version of this technique often suffered from limited sensitivity due to low target copy number or target inaccessibility. In recent years there have been several developments to amend this prob...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Zwirglmaier K

    更新日期:2005-05-15 00:00:00

  • Complete genome sequence of BS49 and draft genome sequence of BS34A, Bacillus subtilis strains carrying Tn916.

    abstract::Bacillus subtilis strains BS49 and BS34A, both derived from a common ancestor, carry one or more copies of Tn916, an extremely common mobile genetic element capable of transfer to and from a broad range of microorganisms. Here, we report the complete genome sequence of BS49 and the draft genome sequence of BS34A, whic...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Browne HP,Anvar SY,Frank J,Lawley TD,Roberts AP,Smits WK

    更新日期:2015-01-01 00:00:00

  • Twitching motility in Legionella pneumophila.

    abstract::Twitching motility is a form of bacterial translocation over solid or semi-solid surfaces mediated by the extension, tethering, and subsequent retraction of type IV pili. These pili are also known to be involved in virulence, biofilm formation, formation of fruiting bodies, horizontal gene transfer, and protein secret...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Coil DA,Anné J

    更新日期:2009-04-01 00:00:00

  • Draft genome sequence of an aromatic compound-degrading bacterium, Desulfobacula sp. TS, belonging to the Deltaproteobacteria.

    abstract::Herein, we report a high-quality draft genome sequence of an uncultivated aromatic compound-degrading bacterium, obtained by the stable isotope probing method from a sulfate-reducing microcosm from an oil-contaminated tidal flat. The obtained genome was closely related with that of Desulfobacula toluolica Tol2. Abunda...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kim SJ,Park SJ,Jung MY,Kim JG,Min UG,Hong HJ,Rhee SK

    更新日期:2014-11-01 00:00:00

  • Conserved and divergent functions of RcrRPQ in Streptococcus gordonii and S. mutans.

    abstract::In the dental caries pathogen Streptococcus mutans, an MarR-like transcriptional regulator (RcrR), two ABC efflux pumps (RcrPQ) and two effector peptides encoded in the rcrRPQ operon provide molecular connections between stress tolerance, (p)ppGpp metabolism and genetic competence. Here, we examined the role of RcrRPQ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shields RC,Burne RA

    更新日期:2015-08-01 00:00:00

  • Test parameters and cell chain length of Streptococcus thermophilus affect the microbial adhesion to hydrocarbons assay: a methodical approach.

    abstract::The microbial adhesion to hydrocarbons (MATH) test is one of the most common method to determine the hydrophobicity of cell surfaces. Despite its prevalence, no standard test parameters are used in literature, making a direct comparison of data almost impossible. Criticism also focuses on test parameters that may mask...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Nachtigall C,Weber C,Rothenburger S,Jaros D,Rohm H

    更新日期:2019-06-01 00:00:00

  • A murine IgG1 monoclonal antibody that binds specifically to outer surface protein A of Lyme disease spirochete Borrelia burgdorferi.

    abstract::A murine monoclonal antibody, designated MA-2G9, directed against outer surface protein A (OspA) of the Lyme disease spirochete, Borrelia burgdorferi, has been produced. Antibody MA-2G9, IgG1 subclass, was purified by affinity chromatography on protein G Sepharose column and used for purification of OspA antigen from ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Abolhassani M,Pavia CS,Dunn JJ,Chiao JW

    更新日期:1993-05-15 00:00:00

  • Impact of the small RNA RyhB on growth, physiology and heterologous protein expression in Escherichia coli.

    abstract::The small noncoding RNA RyhB is a regulator of iron homeostasis in Escherichia coli. During iron limitation, it downregulates the expression of a number of iron-containing proteins, including enzymes of the tricarboxylic acid cycle and the respiratory chain. Because this infers a potential for RyhB to limit energy met...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bollinger CJ,Kallio PT

    更新日期:2007-10-01 00:00:00

  • Biogenic amines in wines: role of lactic acid bacteria.

    abstract::Biogenic amines have undesirable physiological effects when absorbed at too high a concentration. Several kinds of food and beverages contain biogenic amines. Lactic acid bacteria can decarboxylate amino acids. Since winemaking involves the growth of lactic acid bacteria for malolactic fermentation, biogenic amines ma...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Lonvaud-Funel A

    更新日期:2001-05-15 00:00:00

  • Virulence determinants in enteroaggregative Escherichia coli from North India and their interaction in in vitro organ culture system.

    abstract::Enteroaggregative Escherichia coli (EAEC) is an important diarrhoeal pathogen causing diseases in multiple epidemiological and clinical settings. In developing countries like India, diarrhoeal diseases are one of the major killers among paediatric population and oddly, few studies are available from Indian paediatric ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gupta D,Sharma M,Sarkar S,Thapa BR,Chakraborti A

    更新日期:2016-09-01 00:00:00

  • Bacterial gamma-glutamyltranspeptidases: comparison of Escherichia coli and Pseudomonas gamma-glutamyltranspeptidase.

    abstract::A high-level expression system for the Escherichia coli HB101 gamma-glutamyltranspeptidase (GGT) gene was constructed to obtain a sufficient amount of the enzyme for structural studies. About 300 mg of the enzyme was purified from 2 1 of culture broth. Both subunits were needed to reconstitute GGT activity. The E. col...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ishiye M,Niwa M

    更新日期:1992-10-15 00:00:00

  • Survival of nontypeable Haemophilus influenzae in macrophages.

    abstract::In this study we have investigated the ability of nonencapsulated, nontypeable Haemophilus influenzae, NT477 to survive in the J774 mouse macrophage-like cell line. Viable, intracellular nontypeable H. influenzae could still be recovered from macrophages 72 h after phagocytosis. In contrast, H. influenzae strain Rd, a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Craig JE,Cliffe A,Garnett K,High NJ

    更新日期:2001-09-11 00:00:00

  • Bacteria associated with arbuscular mycorrhizal fungi within roots of plants growing in a soil highly contaminated with aliphatic and aromatic petroleum hydrocarbons.

    abstract::Arbuscular mycorrhizal fungi (AMF) belong to phylum Glomeromycota, an early divergent fungal lineage forming symbiosis with plant roots. Many reports have documented that bacteria are intimately associated with AMF mycelia in the soil. However, the role of these bacteria remains unclear and their diversity within intr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Iffis B,St-Arnaud M,Hijri M

    更新日期:2014-09-01 00:00:00

  • Inactivation of the fis gene leads to reduced growth rate.

    abstract::The FIS protein (factor for inversion stimulation) is known to activate the transcription of rRNA and tRNA operons in Escherichia coli. In this report the growth behaviour of a fis bacterium was studied under steady-state conditions and in competition experiments with a wild-type bacterium in a chemostat. The growth r...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Nilsson L,Verbeek H,Hoffmann U,Haupt M,Bosch L

    更新日期:1992-11-15 00:00:00

  • Two types of ion channel formation of tolaasin, a Pseudomonas peptide toxin.

    abstract::Tolaasin is a peptide toxin produced by Pseudomonas tolaasii and causes brown blotch disease of the cultivated mushrooms. Two types of ion channels were identified by the incorporation of tolaasin into lipid bilayer. The slope conductance of type 1 channel measured in the buffer containing 100 mM KCl was 150 pS with a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cho KH,Kim YK

    更新日期:2003-04-25 00:00:00

  • Degradation of Aroclor 1221 in soil by a hybrid pseudomonad.

    abstract::The hybrid Pseudomonas cepacia strain JHR22 was tested for its ability to degrade Aroclor 1221 in soil. The influence of supplements--mineral salts and trace elements--on the degradation was investigated. Disappearance of Aroclor 1221 congeners, occurrence of metabolites, and release of chloride were measured under di...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Havel J,Reineke W

    更新日期:1993-04-01 00:00:00

  • Lactobacillus fermentum 3872 genome sequencing reveals plasmid and chromosomal genes potentially involved in a probiotic activity.

    abstract::In this report we describe a Lactobacillus fermentum 3872 plasmid (pLF3872) not previously found in any other strain of this species. The analysis of the complete sequence of this plasmid revealed the presence of a gene encoding a large collagen-binding protein (CBP), as well as the genes responsible for plasmid maint...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lehri B,Seddon AM,Karlyshev AV

    更新日期:2015-06-01 00:00:00

  • Comparative proteomic and genetic analyses reveal unidentified mutations in Escherichia coli XL1-Blue and DH5α.

    abstract::Escherichia coli has been used widely in laboratory and the biotech industry. However, the genetic and metabolic characteristics remain inadequately studied, particularly for those strains with extensive genetic manipulations that might have resulted in unknown mutations. Here, we demonstrate a comparative proteomics ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Xia XX,Qian ZG,Lee SY

    更新日期:2011-01-01 00:00:00

  • Laboratory-acquired lethal infections by potential bioweapons pathogens including Ebola in 2014.

    abstract::Researchers studying both cellular and viral disease agents in the laboratory have become infected since the early days of microbiology 150 years ago. However, in the early 21st century, new concerns about bioweapons being used to generate terror and also with a series of newly emerging or newly understood disease-cau...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Silver S

    更新日期:2015-01-01 00:00:00

  • A novel type-III staphylococcal cassette chromosome mec (SCCmec) variant among Indian isolates of methicillin-resistant Staphylococcus aureus.

    abstract::We identified a novel type-III staphylococcal cassette chromosome mec (SCCmec) element carried by eight methicillin-resistant Staphylococcus aureus (MRSA) strains from different wards and patients in an Indian hospital. Although the pulsed-field gel electrophoresis pattern and spa types of eight strains were identical...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Arakere G,Nadig S,Ito T,Ma XX,Hiramatsu K

    更新日期:2009-03-01 00:00:00

  • Incorporation of choline into Streptococcus pneumoniae cell wall antigens: evidence for choline kinase activity.

    abstract::The choline-containing teichoic and lipoteichoic acids play an important part in cell wall metabolism of Streptococcus pneumoniae. We propose that a choline kinase enzyme has a role in the synthesis of these antigens. The presence of this enzyme was demonstrated in cell free extracts of S. pneumoniae by measuring the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Whiting GC,Gillespie SH

    更新日期:1996-05-01 00:00:00

  • Light-activated adenyl cyclase from Trichoderma viride.

    abstract::The effect of light on adenyl cyclase (E.C. and 3':5'-cyclic-AMP-phosphodiesterase (E.C. activity of Trichoderma viride was investigated. Adenyl cyclase proved to be a membrane-associated enzyme, requiring Mn2+ and was activated by light. In contrast, 3':5'-cyclic-AMP-phosphodiesterase showed no lig...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kolarova N,Haplová J,Gresík M

    更新日期:1992-06-15 00:00:00

  • Examination of Serpulina pilosicoli for attachment and invasion determinants of Enterobacteria.

    abstract::The spirochaete, Serpulina pilosicoli, is the agent of intestinal spirochaetosis, a diarrhoeal disease of humans and other species. By mechanisms as yet unknown, large numbers of these spirochaetes intimately attach to the colonic mucosa by one cell end. In some infected individuals, the spirochaetes may invade the la...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hartland EL,Mikosza AS,Robins-Browne RM,Hampson DJ

    更新日期:1998-08-01 00:00:00