An improved aminoglycoside resistance gene cassette for use in gram-negative bacteria and Streptomyces.

abstract：:We describe a new procedure allowing the generation and detection of immunogenic antigens from Helicobacter pylori via the hemolysin secretion apparatus of Escherichia coli. The gene (or gene fragment) encoding the H. pylori protein (or protein domain) is inserted in-frame into a residual portion of the hemolysin gene...

journal_title：FEMS microbiology letters

authors： Spreng S,Gentschev I,Goebel W,Mollenkopf H,Eck M,Müller-Hermelink HK,Schmausser B

更新日期：2000-05-15 00:00:00

abstract：:The emergence and dissemination of colistin resistance among Gram-negative bacteria is a global problem. We initiated a surveillance of colistin-resistant and -susceptible Escherichia coli in raw meats from chicken in Nepal. A total of 180 meat samples were collected; from these, 60 E. coli strains were isolated (33.3...

journal_title：FEMS microbiology letters

authors： Joshi PR,Thummeepak R,Leungtongkam U,Pooarlai R,Paudel S,Acharya M,Dhital S,Sitthisak S

更新日期：2019-10-01 00:00:00

abstract：:The identification of virulence factors in Rhodococcus equi has been severely hampered by the lack of a method for in vivo random insertion mutagenesis. This study reports the use of transposomes to generate random insertions of a gene conferring kanamycin resistance into the genome of R. equi ATCC 33701. Southern hyb...

journal_title：FEMS microbiology letters

authors： Mangan MW,Meijer WG

更新日期：2001-12-18 00:00:00

abstract：:Escherichia coli has been used widely in laboratory and the biotech industry. However, the genetic and metabolic characteristics remain inadequately studied, particularly for those strains with extensive genetic manipulations that might have resulted in unknown mutations. Here, we demonstrate a comparative proteomics ...

journal_title：FEMS microbiology letters

authors： Xia XX,Qian ZG,Lee SY

更新日期：2011-01-01 00:00:00

abstract：:Bacterial swarming motility is a flagella-dependent translocation on the surface environment. It has received extensive attention as a population behavior involving numerous genes. Here, we report that Citrobacter freundii, an opportunistic pathogen, exhibits swarming movement on a solid medium surface with appropriat...

journal_title：FEMS microbiology letters

authors： Cong Y,Wang J,Chen Z,Xiong K,Xu Q,Hu F

更新日期：2011-04-01 00:00:00

abstract：:A family of stable lactococcal vectors have been constructed based on the pFX1 replicon using either the alpha fragment or the complete Escherichia coli lacZ gene as a selective marker. These vectors also incorporate multiple cloning sites and examples are given of their use for gene cloning and translational fusion s...

journal_title：FEMS microbiology letters

authors： Xu FF,Pearce LE,Yu PL

更新日期：1991-01-01 00:00:00

abstract：:Saccharomyces cerevisiae was engineered for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum. The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C. ce...

journal_title：FEMS microbiology letters

authors： Hyeon JE,Yu KO,Suh DJ,Suh YW,Lee SE,Lee J,Han SO

更新日期：2010-09-01 00:00:00

abstract：:Five transposon mutants of Francisella novicida were isolated that are compromised in their ability to grow in mouse macrophages in vitro. Sequence analysis of the DNA flanking the transposon insertions identified the genes that were interrupted in these mutants. One of the inactivated loci corresponds to the Francise...

journal_title：FEMS microbiology letters

authors： Gray CG,Cowley SC,Cheung KK,Nano FE

更新日期：2002-09-24 00:00:00

abstract：:Xylose reductase catalyzes the NAD(P)H-dependent reduction of xylose to xylitol and is essential for growth on xylose by yeasts. To understand the nature of coenzyme binding to the Pichia stipitis xylose reductase, we investigated the role of the strictly conserved Lys270 in the putative IPKS coenzyme binding motif by...

journal_title：FEMS microbiology letters

authors： Kostrzynska M,Sopher CR,Lee H

更新日期：1998-02-01 00:00:00

abstract：:Alkyl ether-degrading Rhodococcus sp. strain DEE5151, isolated from activated sewage sludge, has an activity for the oxidation of a variety of alkyl ethers, aralkyl ethers and dibenzyl ether. The whole cell activity for diethyl ether oxidation was effectively inhibited by 2,3-dihydrofurane, ethyl vinyl ether and gluta...

journal_title：FEMS microbiology letters

authors： Kim YH,Engesser KH

更新日期：2005-02-15 00:00:00

abstract：:Immunoassays are increasingly used to investigate the production, properties and fates of the cyanobacterial hepatotoxic microcystins in vitro and in vivo. Responses of an ELISA immunoassay to microcystins have been determined using the authentic toxin antigen, microcystin-LR, and conjugation products between the toxi...

journal_title：FEMS microbiology letters

authors： Metcalf JS,Beattie KA,Pflugmacher S,Codd GA

更新日期：2000-08-15 00:00:00

abstract：:Ninety-three Salmonella isolates recovered from commercial foods and exotic animals in Colombia were studied. The serotypes, resistance profiles and where applicable the quinolone resistance genes were determined. Salmonella Anatum (n=14), Uganda (19), Braenderup (10) and Newport (10) were the most prevalent serovars,...

journal_title：FEMS microbiology letters

authors： Karczmarczyk M,Martins M,McCusker M,Mattar S,Amaral L,Leonard N,Aarestrup FM,Fanning S

更新日期：2010-12-01 00:00:00

abstract：:Truffles are hypogeous Ascomycete fungi belonging to the genus Tuber and forming fruiting bodies highly prized for their taste and aroma. The identification of the genus Tuber and its species is important to investigate their ecology and avoid fraud in the food market. As genus-specific primers are not available, the ...

journal_title：FEMS microbiology letters

authors： Zampieri E,Mello A,Bonfante P,Murat C

更新日期：2009-08-01 00:00:00

abstract：:Biogenic amines have undesirable physiological effects when absorbed at too high a concentration. Several kinds of food and beverages contain biogenic amines. Lactic acid bacteria can decarboxylate amino acids. Since winemaking involves the growth of lactic acid bacteria for malolactic fermentation, biogenic amines ma...

journal_title：FEMS microbiology letters

authors： Lonvaud-Funel A

更新日期：2001-05-15 00:00:00

abstract：:The sepsis caused by Vibrio vulnificus is characterized by an average incubation period of 26 h and a high mortality rate exceeding 50%. The fast growth and dissemination of V. vulnificus in vivo lead to poor clinical outcomes in patients. Therefore, elucidation of the proliferation mechanisms of this organism in vivo...

journal_title：FEMS microbiology letters

authors： Kado T,Kashimoto T,Yamazaki K,Ueno S

更新日期：2017-01-01 00:00:00

abstract：:The effect of low concentrations of cyanide on dissimilatory perchlorate and chlorate reduction and aerobic respiration was examined using pure cultures of Azospira sp. KJ. Cyanide at a concentration of 38 microM inhibited cell growth on perchlorate, chlorate and molecular oxygen, but it did not inhibit the activity o...

journal_title：FEMS microbiology letters

authors： Song Y,Logan BE

更新日期：2004-10-15 00:00:00

abstract：:The effects of exogenously added L-phenylalanine (L-Phe) on the activities of L-phenylalanine ammonia-lyase (PAL) and 3, 4-dimethoxybenzyl alcohol (veratryl alcohol, VA) biosynthesis in ligninolytic cultures of Phanerochaete chrysosporium were investigated. Increasing PAL activity was detected in low nitrogen (LN) cul...

journal_title：FEMS microbiology letters

authors： Hattori T,Nishiyama A,Shimada M

更新日期：1999-10-15 00:00:00

abstract：:Little is known about the diversity and roles of Gram-positive and pigmented bacteria in Antarctic environments, especially those associated with marine macroorganisms. This work is the first study about the diversity and antimicrobial activity of culturable pigmented Gram-positive bacteria associated with marine Anta...

journal_title：FEMS microbiology letters

authors： Leiva S,Alvarado P,Huang Y,Wang J,Garrido I

更新日期：2015-12-01 00:00:00

abstract：:The gene nfxB is one of the genes which affect the cell membrane permeability of quinolones in Pseudomonas aeruginosa PAO. Both wild-type nfxB and a mutant nfxB (nfx13E) were cloned and the DNA sequences were determined. The wild-type gene was dominant in PAO strains. The nfxB mutation was a point mutation (cytosine--...

journal_title：FEMS microbiology letters

authors： Okazaki T,Hirai K

更新日期：1992-10-01 00:00:00

abstract：:Hydrophobin proteins originate from filamentous fungi, which are able to self-assemble at water-air interfaces. Hydrophobins have multiple functions in fungal growth and development. In this study, the function of the Trhfb3 gene encoding a class II hydrophobin was characterized in Trichoderma reesei. The null mutant ...

journal_title：FEMS microbiology letters

authors： He R,Li C,Feng J,Zhang D

更新日期：2017-04-01 00:00:00

abstract：:This commentary describes an assessment exercise known as the TRIPSE (Tri-Partite Problem Solving Exercise) that mimics science in operation. Students frame hypotheses based on limited data, design experiments to test them, which they later revise with new information. It is emphasised that there are no single correct...

journal_title：FEMS microbiology letters

authors： Nastos S,Rangachari PK

更新日期：2016-08-01 00:00:00

abstract：:In the purple sulphur bacterium Allochromatium vinosum, the prosthetic group of dissimilatory sulphite reductase (DsrAB) was identified as siroamide, an amidated form of the classical sirohaem. The genes dsrAB are the first two of a large cluster of genes necessary for the oxidation of sulphur globules stored intracel...

journal_title：FEMS microbiology letters

authors： Lübbe YJ,Youn HS,Timkovich R,Dahl C

更新日期：2006-08-01 00:00:00

abstract：:Two Pseudomonas aeruginosa genes, termed phaJ1(Pa) and phaJ2(Pa), homologous to the Aeromonas caviae (R)-specific enoyl-CoA hydratase gene (phaJ(Ac)) were cloned using a PCR technique to investigate the monomer-supplying ability for polyhydroxyalkanoate (PHA) synthesis from beta-oxidation cycle. Two expression plasmid...

journal_title：FEMS microbiology letters

authors： Tsuge T,Fukui T,Matsusaki H,Taguchi S,Kobayashi G,Ishizaki A,Doi Y

更新日期：2000-03-15 00:00:00

abstract：:Saccharomyces cerevisiae genes encoding functions necessary for inhibition by the Pseudomonas syringae pv. syringae cyclic lipodepsipeptide, syringomycin-E, were identified by mutant analyses. Syringomycin-E-resistant mutants were isolated, shown to contain single recessive mutations, and divided into eight gene compl...

journal_title：FEMS microbiology letters

authors： Takemoto JY,Yu Y,Stock SD,Miyakawa T

更新日期：1993-12-15 00:00:00

abstract：:The production of candidate affinity proteins in a soluble form, for downstream characterization, is often a time-consuming step in combinatorial protein engineering methods. Here, a novel approach for efficient production of candidate clones is described based on direct cleavage of the affinity protein from the surfa...

journal_title：FEMS microbiology letters

authors： Kronqvist N,Löfblom J,Severa D,Ståhl S,Wernérus H

更新日期：2008-01-01 00:00:00

abstract：:CheY, the response regulator of the chemotaxis system in Escherichia coli, can be regulated by two covalent modifications - phosphorylation and acetylation. Both covalent modifications are involved in chemotaxis, but the mechanism and role of the acetylation are still obscure. While acetylation was shown to repress th...

journal_title：FEMS microbiology letters

authors： Li R,Chen P,Gu J,Deng JY

更新日期：2013-10-01 00:00:00

abstract：:Beauveria bassiana is an insect pathogenic fungus widely used as a biocontrol agent to infect and control insect pests. The conidium initiates pathogenesis and mediates disease transmission, however, little is known about genetic elements that control conidiation. Here, the cloning and characterization of a regulatory...

journal_title：FEMS microbiology letters

authors： Fang W,Scully LR,Zhang L,Pei Y,Bidochka MJ

更新日期：2008-02-01 00:00:00

abstract：:Magnetotactic bacteria are microorganisms that respond to magnetic fields. We studied the surface ultrastructure of uncultured magnetotactic cocci collected from a marine environment by transmission electron microscopy using freeze-fracture and freeze-etching. All bacteria revealed a Gram-negative cell wall. Many bact...

journal_title：FEMS microbiology letters

authors： Freitas F,Keim CN,Kachar B,Farina M,Lins U

更新日期：2003-02-14 00:00:00

abstract：:Pseudomonas spp., in particular Pseudomonas fragi, are dominant in aerobically stored chilled meats. This work isolated P. fragi isolates from spoiled chicken and investigated the effect of modified atmosphere packaging (MAP, CO2/N2 30%/70%) on physicochemical properties of P. fragi isolates compared to the correspond...

journal_title：FEMS microbiology letters

authors： Wang GY,Li M,Ma F,Wang HH,Xu XL,Zhou GH

更新日期：2017-06-15 00:00:00

abstract：:A gene encoding a phosphomannose isomerase from Streptococcus mutans GS-5 was identified immediately downstream from the fructokinase gene, scrK. Nucleotide sequence analysis of this region revealed an open reading frame (ORF) specifying a putative protein of 316 amino acids. The gene cloned in Escherichia coli expres...

journal_title：FEMS microbiology letters

authors： Sato Y,Yamamoto Y,Kizaki H,Kuramitsu HK

更新日期：1993-11-15 00:00:00