An improved aminoglycoside resistance gene cassette for use in gram-negative bacteria and Streptomyces.


:A cloning cassette, carrying a modified amino-glycoside resistance gene (neo) from transposon Tn5 was constructed. Three restriction sites internal to the neo gene were eliminated by in vitro mutagenesis, allowing their use in designing new cloning vectors. The original, suboptimal transcription promoter was replaced with a synthetic sequence corresponding to the consensus for E. coli and Streptomyces promoters. The cassette has numerous restriction sites for easy subcloning of the promoter, the coding sequence or the whole gene.


FEMS Microbiol Lett


Denis F,Brzezinski R





Has Abstract


1991-07-01 00:00:00












  • Bioluminescence-based system for rapid detection of natural transformation.

    abstract::Horizontal gene transfer plays a significant role in bacterial evolution and has major clinical importance. Thus, it is vital to understand the mechanisms and kinetics of genetic transformations. Natural transformation is the driving mechanism for horizontal gene transfer in diverse genera of bacteria. Our study intro...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Santala V,Karp M,Santala S

    更新日期:2016-07-01 00:00:00

  • The Serratia-type hemolysin of Chromobacterium violaceum.

    abstract::Chromobacterium violaceum is a Gram-negative opportunistic human pathogen and an inhabitant of tropical soils and waterways. Although known primarily for the synthesis of the pigment violacein, and more recently as a reporter strain for quorum sensing, clinical reports of chromobacteriosis comprise the largest block o...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Brumbach KC,Eason BD,Anderson LK

    更新日期:2007-02-01 00:00:00

  • Mycoplasma gallisepticum rpoA gene cluster.

    abstract::Two conservative gene clusters, the S10 ribosomal protein region and one (of the two) set of rRNA genes, were split in a genome crossover rearrangement event in Mycoplasma gallisepticum. As a result of the rearrangement the major part of the S10 ribosomal protein cluster is located upstream of genes for 23S-5S rRNA (r...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Skamrov A,Feoktistova E,Goldman M,Beabealashvilli R

    更新日期:2002-03-05 00:00:00

  • Use of a microarray to assess the distribution of plasmid and chromosomal virulence genes in strains of enteroaggregative Escherichia coli.

    abstract::A DNA microarray was used to analyze the distribution of plasmid and chromosomal genes among strains of enteroaggregative Escherichia coli (EAEC) isolated from a prospective diarrhoea surveillance study in the United Kingdom. Target genes were extracted from existing databases and from the genome sequence of prototype...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jenkins C,van Ijperen C,Dudley EG,Chart H,Willshaw GA,Cheasty T,Smith HR,Nataro JP

    更新日期:2005-12-01 00:00:00

  • Description of two new species of Aliinostoc and one new species of Desmonostoc from India based on the Polyphasic Approach and reclassification of Nostoc punensis to Desmonostoc punense comb. nov.

    abstract::Three heterocytous cyanobacterial strains were isolated from different habitats of Central India, and initial morphological studies indicated them to be members of the genus Nostoc or other closely related genera. Subsequent studies using morphological, ecological, molecular and phylogenetic methods indicated the thre...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Saraf A,Dawda HG,Suradkar A,Behere I,Kotulkar M,Shaikh ZM,Kumat A,Batule P,Mishra D,Singh P

    更新日期:2018-12-01 00:00:00

  • Free-living freshwater amoebae differ in their susceptibility to the pathogenic bacterium Legionella pneumophila.

    abstract::Legionella pneumophila is known as a facultative intracellular parasite of free-living soil and freshwater amoebae, of which several species have been shown to support the growth of the pathogenic bacteria. We report for the first time the behaviour of two strains (c2c and Z503) of the amoeba Willaertia magna towards ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dey R,Bodennec J,Mameri MO,Pernin P

    更新日期:2009-01-01 00:00:00

  • Neisseria meningitidis transferrin-binding protein 1 expressed in Escherichia coli is surface exposed and binds human transferrin.

    abstract::A gene library of Neisseria meningitidis B15 P1.16 DNA was established in lambda Zap II and clones containing DNA encoding transferrin binding protein 1 (TBP-1) identified following hybridisation with a 63-bp DNA probe based on the codon assignment for the first 21 N-terminal amino acids of TBP-1. Sequencing of the cl...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Palmer HM,Powell NB,Ala'Aldeen DA,Wilton J,Borriello SP

    更新日期:1993-06-15 00:00:00

  • An endophytic/pathogenic Phoma sp. from creosote bush producing biologically active volatile compounds having fuel potential.

    abstract::A Phoma sp. was isolated and characterized as endophytic and as a pathogen of Larrea tridentata (creosote bush) growing in the desert region of southern Utah, USA. This fungus produces a unique mixture of volatile organic compounds (VOCs), including a series of sesquiterpenoids, some alcohols and several reduced napht...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Strobel G,Singh SK,Riyaz-Ul-Hassan S,Mitchell AM,Geary B,Sears J

    更新日期:2011-07-01 00:00:00

  • Analysis of the Spirochaeta aurantia flaA gene and transcript.

    abstract::The flaA gene, which codes for the Spirochaeta aurantia flagellar filament outer layer polypeptide, FlaA, was cloned, sequenced and analysed. The gene appears to be transcribed into a monocistronic mRNA from a sigma 70-like promoter. The translational start is 31 base pairs after the start of the transcript, the open ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Parales J Jr,Greenberg EP

    更新日期:1993-02-01 00:00:00

  • Purification and partial characterization of an antigen specific to Lactobacillus brevis strains with beer spoilage activity.

    abstract::Certain Lactobacillus brevis strains are resistant to hop-derived compounds such as isohumulone and are able to grow in beer. In this study, we raised an antiserum against our beer spoilage laboratory strain L. brevis 578 which reacted with 23 of 24 beer spoilers and two of 13 non-spoilers in precipitation reactions u...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Yasui T,Yoda K

    更新日期:1997-06-15 00:00:00

  • Agricultural production systems can serve as reservoir for human pathogens.

    abstract::Food-borne diseases are a threat to human health and can cause severe economic losses. Nowadays, in a growing and increasingly interconnected world, food-borne diseases need to be dealt with in a global manner. In order to tackle this issue, it is essential to consider all possible entry routes of human pathogens into...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Schierstaedt J,Grosch R,Schikora A

    更新日期:2019-12-01 00:00:00

  • Specific interaction of the protein-D2 porin of Pseudomonas aeruginosa with antibiotics.

    abstract::The protein-D2 porin of Pseudomonas aeruginosa is lacking in carbapenem or fluoroquinolone-resistant strains and hence was thought to facilitate the diffusion of these antibiotics. We examined the effect of several antibiotics on the single channel conductivity of protein-D2 in planar lipid bilayers and found that flu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ishii J,Nakae T

    更新日期:1996-02-01 00:00:00

  • Acetaldehyde dehydrogenase activity of the AdhE protein of Escherichia coli is inhibited by intermediates in ubiquinone synthesis.

    abstract::Defects in the acd gene (which may be allelic to ubiH) result in the inactivation of the coenzyme A-linked acetaldehyde dehydrogenase activity of the multifunctional AdhE protein of Escherichia coli. This activity is restored by addition of ubiquinone-0 to cell extracts. However, the alcohol dehydrogenase activity of ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gupta S,Mat-Jan F,Latifi M,Clark DP

    更新日期:2000-01-01 00:00:00

  • In vitro assay for the Bacillus subtilis signal peptidase SipS: systems for efficient in vitro transcription-translation and processing of precursors of secreted proteins.

    abstract::The signal peptidase (SPase) SipS of Bacillus subtilis is responsible for the processing of precursors of secreted proteins. It differs from the SPases of Gram-negative bacteria in structure and specificity. To assay the activity of SipS in vitro, two efficient transcription-translation systems for the synthesis of ra...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Vehmaanperä J,Görner A,Venema G,Bron S,van Dijl JM

    更新日期:1993-12-01 00:00:00

  • Microorganisms form exocellular structures, trophosomes, to facilitate biodegradation of oil in aqueous media.

    abstract::Cytochemical staining and microscopy were used to study the trophic structures and cellular morphotypes that are produced during the colonization of oil-water interfaces by oil-degrading yeasts and bacteria. Among the microorganisms studied here, the yeasts (Schwanniomyces occidentalis, Torulopsis candida, Candida tro...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dmitriev VV,Crowley D,Rogachevsky VV,Negri CM,Rusakova TG,Kolesnikova SA,Akhmetov LI

    更新日期:2011-02-01 00:00:00

  • Construction of a fusion protein carrying antigenic determinants of enteric clostridial toxins.

    abstract::Clostridium difficile and Clostridium perfringens type A are infectious agents of enteric diseases. The main virulence factors of these microorganisms include toxins A and B of C. difficile (ToxA and ToxB) and enterotoxin of C. perfringens (Cpe). In this study genetic constructions have been created for the expression...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Belyi IF,Varfolomeeva NA

    更新日期:2003-08-29 00:00:00

  • A novel computerised image analysis method for the measurement of production of conidia from the aphid pathogenic fungus Erynia neoaphidis.

    abstract::A semi-automated method has been developed for the quantification and measurement of conidia discharged by the aphid pathogen Erynia neoaphidis. This was used to compare conidiation by E. neoaphidis-mycosed pea aphid cadavers, mycelial plugs cut from agar plates, mycelial pellets from shake flasks and by mycelial pell...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bonner TJ,Pell JK,Gray SN

    更新日期:2003-03-14 00:00:00

  • Has the prehistoric ice-man contributed to the preservation of living fungal spores?

    abstract::There are arguments against the conclusion drawn by Haselwandter and Ebner that fungal spores have survived for some 5300 years on hay padding in the leather boots of a frozen body discovered in the Austrian Alps. According to cryobiological experience, long-term survival of fungal spores is very unlikely at temperatu...

    journal_title:FEMS microbiology letters

    pub_type: 评论,杂志文章


    authors: Gams W,Stalpers JA

    更新日期:1994-07-01 00:00:00

  • A new biotype of Fusarium oxysporum f. sp. lycopersici race 2 emerged by a transposon-driven mutation of avirulence gene AVR1.

    abstract::Emergence of races in Fusarium oxysporum f. sp. lycopersici (Fol) is caused by loss or mutation of at least one avirulence (AVR) gene. The product of AVR1 is a small protein (Avr1) secreted by Fol in tomato xylem sap during infection. This protein triggers Fol race 1 specific resistance (I) in tomato, indicating that ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kashiwa T,Suzuki T,Sato A,Akai K,Teraoka T,Komatsu K,Arie T

    更新日期:2016-07-01 00:00:00

  • Use of intersimple sequence repeats markers to develop strain-specific SCAR markers for Lentinula edodes.

    abstract::Although Lentinula edodes is the second most important cultivated mushroom worldwide, most industrially cultivated strains have been identified only through traditional phenotypic analysis. Here, we report for the first time the use of sequence characterized amplified region (SCAR) markers for strain differentiation. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Qin LH,Tan Q,Chen MJ,Pan YJ

    更新日期:2006-04-01 00:00:00

  • Borrelia burgdorferi possesses a collagenolytic activity.

    abstract::Lyme disease is a multisystemic disorder caused by Borrelia burgdorferi, an invasive spirochete. B. burgdorferi has a predilection for collagenous tissue and one major clinical manifestation of the disease is arthritis. We have identified a collagenolytic activity in B. burgdorferi detergent lysates using iodinated ge...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Grab DJ,Kennedy R,Philipp MT

    更新日期:1996-10-15 00:00:00

  • Expression of the mphB gene for macrolide 2'-phosphotransferase II from Escherichia coli in Staphylococcus aureus.

    abstract::The genes mphA and mphB encode macrolide 2'-phosphotransferases I and II, respectively, and they confer resistance to macrolide antibiotics in Escherichia coli. To study the expression of these genes in Gram-positive bacteria, we constructed recombinant plasmids that consisted of an mph gene and the pUB110 vector in B...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Noguchi N,Tamura Y,Katayama J,Narui K

    更新日期:1998-02-15 00:00:00

  • Effect of Mg(2+) ion in protein secretion by magnesium-resistant strains of Pseudomonas aeruginosa and Vibrio parahaemolyticus isolated from the coastal water of Haldia port.

    abstract::A rapidly growing industrial complex including oil refineries and chemical industries has developed around the coastal area of Haldia port in the district of Midnapore, West Bengal, India. The coastal water is highly polluted with industrial wastes along with petroleum hydrocarbons. The bacteria isolated from the diff...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bhattacharya M,Roy SS,Biswas D,Kumar R

    更新日期:2000-04-15 00:00:00

  • Dark chocolate as a stable carrier of microencapsulated Akkermansia muciniphila and Lactobacillus casei.

    abstract::The viability of probiotics is affected by several factors during manufacturing, storage and gastrointestinal tract passage. Protecting the probiotics from harmful conditions is particularly critical for oxygen sensitive species like Akkermansia muciniphila, a bacterium which recently has been proposed as a next-gener...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Marcial-Coba MS,Saaby L,Knøchel S,Nielsen DS

    更新日期:2019-01-01 00:00:00

  • Community structure in a methanotroph biofilter as revealed by phospholipid fatty acid analysis.

    abstract::The microbial community structure of two biofilters used for the oxidation of methane and organic trace gases generated in landfills was analysed by phospholipid fatty acid composition. Community structure varied with biofilter depth, reflecting varying conditions of substrate supply as well as of organic carbon conte...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gebert J,Gröngröft A,Schloter M,Gattinger A

    更新日期:2004-11-01 00:00:00

  • In vitro conjugal transfer of tetracycline resistance from Lactobacillus isolates to other Gram-positive bacteria.

    abstract::The ability of 14 Lactobacillus strains, isolated from fermented dry sausages, to transfer tetracycline resistance encoded by tet(M) through conjugation was examined using filter mating experiments. Seven out of 14 tetracycline-resistant Lactobacillus isolates were able to transfer in vitro this resistance to Enteroco...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gevers D,Huys G,Swings J

    更新日期:2003-08-08 00:00:00

  • D-Cycloserine biases enrichment for auxotrophic mutants of Zymomonas mobilis.

    abstract::Contrary to its effect on rich medium, D-cycloserine showed no bactericidal effect on Zymomonas mobilis cells cultured on mineral medium. Addition of a mixture of glycine and glutamic acid to the mineral medium restored its bactericidal action. However, mutant enrichments run in these conditions were biased, with most...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Pencreac'h G,Aït-Abdelkader N,Joset F,Baratti JC

    更新日期:1996-09-01 00:00:00

  • Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar Typhimurium.

    abstract::The RcsCDB (Rcs) phosphorelay system regulates capsule synthesis, flagella production and other cellular activities in several enteric bacteria. This system consists of three proteins: the sensor RcsC, the cognate response regulator RcsB and the histidine-containing phosphotransfer protein RcsD (YojN), which is hypoth...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Pescaretti Mde L,Morero R,Delgado MA

    更新日期:2009-11-01 00:00:00

  • Mechanisms of protein kinase Sch9 regulating Bcy1 in Saccharomyces cerevisiae.

    abstract::In this study, we investigated the mechanisms of Sch9 regulating the localization and phosphorylation of Bcy1. Our research indicated that Sch9 regulated localization of Bcy1 via Zds1 for the following reasons: (1) deletions of SCH9 or ZDS1 both caused nuclear localization of Bcy1; (2) Sch9 and Zds1 interacted physica...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Zhang A,Gao W

    更新日期:2012-06-01 00:00:00

  • Molecular characterization of beta-lactamase genes blaA and blaB of Yersinia enterocolitica biovar 1A.

    abstract::The beta-lactamase genes blaA and blaB were detected by PCR amplification in strains of Yersinia enterocolitica biovar 1A isolated from India, Germany, France and the USA. Both genes were detected in all strains. Polymerase chain reaction-restriction fragment length polymorphism revealed genetic heterogeneity in blaA ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sharma S,Mittal S,Mallik S,Virdi JS

    更新日期:2006-04-01 00:00:00