Construction of a family of lactococcal vectors for gene cloning and translational fusion.

Abstract:

:A family of stable lactococcal vectors have been constructed based on the pFX1 replicon using either the alpha fragment or the complete Escherichia coli lacZ gene as a selective marker. These vectors also incorporate multiple cloning sites and examples are given of their use for gene cloning and translational fusion studies in lactococci.

journal_name

FEMS Microbiol Lett

authors

Xu FF,Pearce LE,Yu PL

doi

10.1111/j.1574-6968.1991.tb04321.x

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

55-9

issue

1

eissn

0378-1097

issn

1574-6968

journal_volume

61

pub_type

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