Kluyveromyces wickerhamii killer toxin: purification and activity towards Brettanomyces/Dekkera yeasts in grape must.

abstract：:Fire blight, caused by plant pathogenic bacterium Erwinia amylovora, is one of the most important diseases of Rosaceae plants. Due to the lack of effective control measures, fire blight infections pose a recurrent threat on agricultural production worldwide. Recently, bacterial viruses, or bacteriophages, have been pr...

journal_title：FEMS microbiology letters

authors： Besarab NV,Akhremchuk AE,Zlatohurska MA,Romaniuk LV,Valentovich LN,Tovkach FI,Lagonenko AL,Evtushenkov AN

更新日期：2020-05-01 00:00:00

abstract：:It is often claimed that Antoni van Leeuwenhoek was secretive about his methods. However, closer examination of his letters suggests that this reputation was not always deserved. Some letters were not published in the Royal Society Proceedings, and others were edited. This paper describes the repetition of his experim...

journal_title：FEMS microbiology letters

authors： Robertson LA

更新日期：2019-08-01 00:00:00

abstract：:The outer membrane protein (OMP) profiles of four different strains of Bacteroides fragilis, as determined by Coomassie blue stained polyacrylamide gels, were compared after growth in broth culture and in the mouse peritoneal cavity. There was no induction of the expression of large quantities of novel OMP after growt...

journal_title：FEMS microbiology letters

authors： Patrick S,Lutton DA

更新日期：1990-09-01 00:00:00

abstract：:E7 oncoprotein of human papillomavirus-16 (HPV-16) is constitutively produced in cervical cancer (CxCa) and is a good candidate for the design of therapeutic vaccines. In this work, the nisin-controlled expression system was used to display the E7 protein at the cell surface of the food-grade Gram-positive bacterium L...

journal_title：FEMS microbiology letters

authors： Cortes-Perez NG,Bermúdez-Humarán LG,Le Loir Y,Rodriguez-Padilla C,Gruss A,Saucedo-Cárdenas O,Langella P,Montes-de-Oca-Luna R

更新日期：2003-12-05 00:00:00

abstract：:The ADAMs are a family of integral membrane proteases involved in shedding and fusion events in animal tissues. Here, we report the identification of two ADAMs, ADM-A and ADM-B, in the pathogenic fungus Aspergillus fumigatus. The domain structure of metazoan ADAMs was seen in ADM-A and -B, although with some differenc...

journal_title：FEMS microbiology letters

authors： Lavens SE,Rovira-Graells N,Birch M,Tuckwell D

更新日期：2005-07-01 00:00:00

abstract：:Endpolygalacturonase I from Stereum purpureum has been identified as a causative substance for the silver-leaf disease in apples. It possesses a unique pro-sequence in the C-terminal region that lacks endpolygalacturonases from any other origin. In this study, we analyzed and compared enzymatic characteristics between...

journal_title：FEMS microbiology letters

authors： Hamada S,Toda K,Ogawa S,Kubota K,Miyairi K

更新日期：2015-09-01 00:00:00

abstract：:The beta-lactamase genes blaA and blaB were detected by PCR amplification in strains of Yersinia enterocolitica biovar 1A isolated from India, Germany, France and the USA. Both genes were detected in all strains. Polymerase chain reaction-restriction fragment length polymorphism revealed genetic heterogeneity in blaA ...

journal_title：FEMS microbiology letters

authors： Sharma S,Mittal S,Mallik S,Virdi JS

更新日期：2006-04-01 00:00:00

abstract：:The assessment of scientific research is essentially based on several metric parameters, the so-termed Impact Factor perhaps being the predominant one. Despite well-founded criticisms and the wide opposition of reputed scientists, this procedure has become a tool of scientific policy, and is applied in editorial proce...

journal_title：FEMS microbiology letters

authors： Argüelles JC,Argüelles-Prieto R

更新日期：2019-06-01 00:00:00

abstract：:Salicylate was found to uniquely induce a 27-kDa protein in Mycobacterium tuberculosis complex organisms but not in Mycobacterium smegmatis or Escherichia coli. The structural analogue antitubercular para-amino-salicylate also induced the 27-kDa protein but to a somewhat lower level than salicylate. Other structural a...

journal_title：FEMS microbiology letters

authors： Sun Z,Cheng SJ,Zhang H,Zhang Y

更新日期：2001-09-25 00:00:00

abstract：:How proteins reach their native conformation and location has been a major question of biology during the last 50 years. To counterbalance protein misfolding and the accumulation of aggregation products, a complex network of chaperones and proteases takes care of protein quality control in the cell. Such a chaperone n...

journal_title：FEMS microbiology letters

authors： Mas G,Hiller S

更新日期：2018-07-01 00:00:00

abstract：:In Mycobacterium tuberculosis (MTB), rifampicin resistance is almost invariably due to mutations in the rpoB gene, whose function is critical for cell viability. Most of these mutations, at least initially, impair the fitness of the bacteria but confer a selective advantage when antibiotic pressure is exerted. Subsequ...

journal_title：FEMS microbiology letters

authors： Bergval IL,Klatser PR,Schuitema AR,Oskam L,Anthony RM

更新日期：2007-10-01 00:00:00

abstract：:Antibiotic therapies are known to disrupt gastrointestinal (GI) bacterial communities. HIV and pathogenic simian immunodeficiency virus (SIV) infections have also been associated with disrupted GI bacterial communities. We administered a combination antibiotic therapy to six SIV-infected rhesus macaques and collected ...

journal_title：FEMS microbiology letters

authors： Zevin AS,Hensley-McBain T,Miller C,Smith E,Langevin S,Klatt NR

更新日期：2017-12-15 00:00:00

abstract：:Cholera epidemics caused by Vibrio cholerae 01 continue to represent a major public health concern in many developing countries. A rapid and simple test kit for the detection of V. cholerae 01 has been developed. The kit, CholeraScreen is a monoclonal antibody-based, co-agglutination test and is used directly with sto...

journal_title：FEMS microbiology letters

authors： Colwell RR,Hasan JA,Huq A,Loomis L,Siebeling RJ,Torres M,Galvez S,Islam S,Tamplin MT,Bernstein D

更新日期：1992-10-15 00:00:00

abstract：:Xylose reductase catalyzes the NAD(P)H-dependent reduction of xylose to xylitol and is essential for growth on xylose by yeasts. To understand the nature of coenzyme binding to the Pichia stipitis xylose reductase, we investigated the role of the strictly conserved Lys270 in the putative IPKS coenzyme binding motif by...

journal_title：FEMS microbiology letters

authors： Kostrzynska M,Sopher CR,Lee H

更新日期：1998-02-01 00:00:00

abstract：:The aim of this study was the production of the homopolyester poly(4-hydroxybutyric acid) (poly(4HB)) with recombinant strains of Escherichia coli. Wild-type strains and other widely used non-recombinant strains of E. coli are not able to produce polyhydroxyalkanoic acids (PHA) as storage compounds and cannot utilize ...

journal_title：FEMS microbiology letters

authors： Hein S,Söhling B,Gottschalk G,Steinbüchel A

更新日期：1997-08-15 00:00:00

abstract：:The presence and distribution of isoprenoid glycerol dialkyl glycerol tetraethers (GDGTs), lipids that constitute the membranes of Archaea, have been investigated in a 50-cm long core from a Swedish peat bog. In the acrotelm, the periodically water saturated and thus oxic upper layer of the peat bog, only minor amount...

journal_title：FEMS microbiology letters

authors： Weijers JW,Schouten S,van der Linden M,van Geel B,Damsté JS

更新日期：2004-10-01 00:00:00

abstract：:Escherichia coli mutant MM52 (secA(ts)) was transformed with a cosmid library from Staphylococcus carnosus, and a recombinant cosmid (pBO23) allowing growth at the non-permissive temperature (42 degrees C) was isolated. pBO23 also restored the growth defects of E. coli mutants IQ85 (secY(ts)) and IT41 (lep(ts)). Nucle...

journal_title：FEMS microbiology letters

authors： Overhoff-Freundlieb B,Freudl R

更新日期：1991-11-15 00:00:00

abstract：:The replication region of plasmid pRA2 from Pseudomonas alcaligenes NCIB 9867 (strain P25X) was localized within a 5.9-kbp DNA fragment and its sequence was determined. An interesting feature of the sequence is the presence of a 1.3-kbp region containing seven, highly conserved, direct repeats of 72 bp in length. The ...

journal_title：FEMS microbiology letters

authors： Kwong SM,Yeo CC,Chuah D,Poh CL

更新日期：1998-01-15 00:00:00

abstract：:Loop-mediated isothermal amplification (LAMP) assay detected Salmonella within 60 min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella. The sensitivity of the LAMP assay w...

journal_title：FEMS microbiology letters

authors： Hara-Kudo Y,Yoshino M,Kojima T,Ikedo M

更新日期：2005-12-01 00:00:00

abstract：:We present draft genome sequences of three Holospora species, hosted by the ciliate Paramecium caudatum; that is, the macronucleus-specific H. obtusa and the micronucleus-specific H. undulata and H. elegans. We investigate functions of orthologous core genes conserved across the three Holospora species, which may be e...

journal_title：FEMS microbiology letters

authors： Dohra H,Tanaka K,Suzuki T,Fujishima M,Suzuki H

更新日期：2014-10-01 00:00:00

abstract：:It is often taken for granted that all animals host and depend upon a microbiome, yet this has only been shown for a small proportion of species. We propose that animals span a continuum of reliance on microbial symbionts. At one end are the famously symbiont-dependent species such as aphids, humans, corals and cows, ...

journal_title：FEMS microbiology letters

authors： Hammer TJ,Sanders JG,Fierer N

更新日期：2019-05-01 00:00:00

abstract：:To investigate the regulatory mechanism governing antifungal metabolite biosynthesis, two kinds of global regulator genes in Pseudomonas sp. M18, an rpoS and an rsmA gene, were cloned and mutated by inserting with an aacC1 cassette, respectively. Two mutants showed the same regulatory mode with repression of phenazine...

journal_title：FEMS microbiology letters

authors： Ge Y,Yang S,Fang Y,Yang R,Mou D,Cui J,Wen L

更新日期：2007-03-01 00:00:00

abstract：:The sequence of 1383 nucleotides of the DNA encoding 16S rDNA was determined for strains of human intestinal spirochaetes, comprising an unnamed isolate and "Brachyspira aalborgi" NCTC 11492. A phylogenetic tree was inferred from aligned sequence comparisons between the intestinal spirochaetes, representatives of the ...

journal_title：FEMS microbiology letters

authors： Hookey JV,Barrett SP,Reed CS,Barber P

更新日期：1994-04-15 00:00:00

abstract：:In order to characterize the metabolites produced in vivo by biphenyl-2,3-dioxygenase and biphenyl-2,3-dihydrodiol-2,3-dehydrogenase, the first two enzymes of the (polychloro)biphenyl catabolic pathway encoded by the bph locus of Pseudomonas sp. LB400, recombinant E. coli strains expressing the respective genes were c...

journal_title：FEMS microbiology letters

authors： Seeger M,Timmis KN,Hofer B

更新日期：1995-11-15 00:00:00

abstract：:A unique genomic DNA fragment was isolated from Nitrosomonas europaea ATCC 19718. Based on the sequence of this fragment, oligonucleotide primers for polymerase chain reaction amplification were prepared which amplify sequences of 775 and 658 bp. The predicted DNA fragments were both amplified from the genome of N. eu...

journal_title：FEMS microbiology letters

authors： Nejidat A,Abeliovich A

更新日期：1994-07-01 00:00:00

abstract：:Rhodopirellula baltica (strain SH 1T) is a free-living marine representative of the phylogenetically independent and environmentally relevant phylum Planctomycetes. Little is known about the regulatory strategies of free-living bacteria with large (7.15 Mb) genomes. Therefore, a consistent, quantitative and qualitativ...

journal_title：FEMS microbiology letters

authors： Lombardot T,Bauer M,Teeling H,Amann R,Glöckner FO

更新日期：2005-01-01 00:00:00

abstract：:The bovine enteric calici-like virus, Newbury agent 1 (NA1) was characterised to determine if it is a member of the Caliciviridae and to establish its antigenic relationship to the established bovine enteric calicivirus Newbury agent 2 (NA2). Solid phase immune electron microscopy (SPIEM) allowed quantification of NA1...

journal_title：FEMS microbiology letters

authors： Dastjerdi AM,Snodgrass DR,Bridger JC

更新日期：2000-11-01 00:00:00

abstract：:Changes in composition of the principal low molecular mass thiols of Leishmania donovani were monitored during the transformation of promastigotes, first to stationary phase metacyclic forms and then to amastigotes. No consistent variation in the thiol composition of the parasite which could account for the known incr...

journal_title：FEMS microbiology letters

authors： Weldrick DP,Chodacka B,Vogt R,Steenkamp DJ

更新日期：1999-04-01 00:00:00

abstract：:Three strains of Streptococcus uberis isolated from dairy cows with mastitis were co-cultured with a bovine mammary epithelial cell line (MAC-T) in Dulbecco's modified Eagle's medium without fetal bovine serum. Protein profiles from culture supernatants and bacterial pellets among different treatments were compared by...

journal_title：FEMS microbiology letters

authors： Fang W,Luther DA,Oliver SP

更新日期：1998-09-15 00:00:00

abstract：:Isolated membranes of the extreme haloalkaliphilic archaeon Natronococcus occultus were able to hydrolyze ATP via an ATPase, which required the presence of Mg(2+), high concentrations of NaCl, and a pH value of 9. The native molecular mass of the purified ATPase was 130 kDa and was composed of 74- and 61-kDa subunits....

journal_title：FEMS microbiology letters

authors： Eddy ML,Jablonski PE

更新日期：2000-08-15 00:00:00