Abstract:
:Salicylate was found to uniquely induce a 27-kDa protein in Mycobacterium tuberculosis complex organisms but not in Mycobacterium smegmatis or Escherichia coli. The structural analogue antitubercular para-amino-salicylate also induced the 27-kDa protein but to a somewhat lower level than salicylate. Other structural analogues such as benzoic acid and acetyl salicylic acid (aspirin) did not induce the 27-kDa protein. Western blot analysis indicated that the 27-kDa protein was localized mainly in the cytoplasm. The 27-kDa protein was not expressed at different growth phases in the absence of salicylate. The 27-kDa protein was identified as a putative benzoquinone methyltransferase (Rv0560c), which has several homologues in the M. tuberculosis genome. The cloned 27-kDa gene was found to express constitutively in E. coli, M. smegmatis and BCG with or without salicylate.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Sun Z,Cheng SJ,Zhang H,Zhang Ydoi
10.1111/j.1574-6968.2001.tb10843.xkeywords:
subject
Has Abstractpub_date
2001-09-25 00:00:00pages
211-6issue
2eissn
0378-1097issn
1574-6968pii
S037810970100355Xjournal_volume
203pub_type
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