Production of biobutanol from cellulose hydrolysate by the Escherichia coli co-culture system.

abstract：:Two fungal isolates, formerly classified as Verticillium alboatrum and proposed as forming the basis of a new sub-group ('Group 2') within the species, have been shown to be non-pathogenic to known hosts of V. alboatrum and, on the basis of molecular evidence, to be closely related to Verticillium psalliotae and Verti...

journal_title：FEMS microbiology letters

authors： Carder JH,Barbara DJ

更新日期：1999-01-01 00:00:00

abstract：:A genomic bank was constructed in Escherichia coli HB101, consisting of DNA fragments from Bacteroides thetaiotaomicron strain 489 inserted within the vector pBR322. By screening on complex medium containing blue dextran, 10 stable dextranase-positive (Dex+) clones were isolated. Seven groups of Dex+ inserts were iden...

journal_title：FEMS microbiology letters

authors： Joncquiert JC,Béchet M,Tierny Y,Courtois J,Dubourguier HC,Guillaume JB

更新日期：1991-12-01 00:00:00

abstract：:A complementary DNA of the Aspergillus nidulans chsB gene encoding chitin synthase, an essential gene for hyphal growth, was obtained by RT-PCR and expressed in Saccharomyces cerevisiae by using the GAL1 promoter in a multicopy plasmid. The biochemical characteristics of chitin synthase B (ChsB) expressed in S. cerevi...

journal_title：FEMS microbiology letters

authors： Tatsuno K,Yamada-Okabe H,Takagi M,Arisawa M,Sudoh M

更新日期：1997-04-15 00:00:00

abstract：:The mosquitocidal crystal protein, Cry19Aa, from Bacillus thuringiensis ssp. jegathesan, has high toxicity to Anopheles stephensi and Culex pipiens but is less toxic to Aedes aegypti. To study the functional role of putative domain II and surface residues in mosquito toxicity, 16 alanine substitution mutations were in...

journal_title：FEMS microbiology letters

authors： Roh JY,Nair MS,Liu XS,Dean DH

更新日期：2009-06-01 00:00:00

abstract：:We transformed a clinical Staphylococcus epidermidis isolate with the Enterococcus faecalis transposon Tn917-carrying plasmid pTV1. Loss of plasmid replication was observed at 47 degrees C. Tn917 transposes efficiently and apparently randomly. The transposition frequency could be stimulated by erythromycin. Transposon...

journal_title：FEMS microbiology letters

authors： Grueter L,Koenig O,Laufs R

更新日期：1991-08-01 00:00:00

abstract：:Stress-activated MAPK pathways are systems used to regulate the stress adaptation of most fungi. It has been shown that in Talaromyces marneffei (Penicillium marneffei), a pathogenic dimorphic fungus, the sakA gene is involved, not only in tolerance against oxidative and heat stresses, but also in playing a role in as...

journal_title：FEMS microbiology letters

authors： Nimmanee P,Tam EWT,Woo PCY,Vanittanakom P,Vanittanakom N

更新日期：2017-04-01 00:00:00

abstract：:Growth of Pseudomonas aeruginosa on acyclic terpene alcohols (citronellol) and on other methyl-branched compounds such as leucine or isovalerate requires a functional leucine/isovalerate utilization (Liu) pathway. In this study, we investigated the liuABCDE gene cluster by insertion mutant analysis, heterologous expre...

journal_title：FEMS microbiology letters

authors： Förster-Fromme K,Jendrossek D

更新日期：2008-09-01 00:00:00

abstract：:The phylogenetic position of an acidophilic chemo-organotrophic menaquinone-containing bacterium, Acidobacterium capsulatum, was studied on the basis of 16S rRNA gene sequence information. A. capsulatum showed the highest level of sequence similarity to Heliobacterium chlorum, a member of the Gram-positive group, yet ...

journal_title：FEMS microbiology letters

authors： Hiraishi A,Kishimoto N,Kosako Y,Wakao N,Tano T

更新日期：1995-10-01 00:00:00

abstract：:The devR-devS two-component system of Mycobacterium tuberculosis was identified earlier and partially characterized in our laboratory. A devR::kan mutant of M. tuberculosis was constructed by allelic exchange. The devR mutant strain showed reduced cell-to-cell adherence in comparison to the parental strain in laborato...

journal_title：FEMS microbiology letters

authors： Malhotra V,Sharma D,Ramanathan VD,Shakila H,Saini DK,Chakravorty S,Das TK,Li Q,Silver RF,Narayanan PR,Tyagi JS

更新日期：2004-02-16 00:00:00

abstract：:The emergence of antibiotic resistance has necessitated new therapeutic approaches for combating persistent bacterial infection. An alternative approach is regulation of bacterial virulence instead of growth suppression, which can readily lead to drug resistance. The virulence of the opportunistic human pathogen Pseud...

journal_title：FEMS microbiology letters

authors： Lee JH,Kim YG,Cho MH,Kim JA,Lee J

更新日期：2012-04-01 00:00:00

abstract：:Strains of Salmonella enteritidis expressed a novel porin with a subunit size of 35.5 kDa as shown by SDS-PAGE. This protein was expressed as a major outer membrane protein (MOMP) when grown on nutrient agar, McConkey agar or blood agar, or in Tris-succinate medium; but was only produced in trace amounts when strains ...

journal_title：FEMS microbiology letters

authors： Chart H,Frost JA,Rowe B

更新日期：1993-05-15 00:00:00

abstract：:Many bacteria are capable of accumulating intracellular granules of polyhydroxyalkanoates (PHA). In this work, we developed confocal microscopy analysis of bacterial cells to study changes in the diameters of cells as well as PHA granules during growth and PHA accumulation in the bacterium Cupriavidus necator H16 (for...

journal_title：FEMS microbiology letters

authors： Mravec F,Obruca S,Krzyzanek V,Sedlacek P,Hrubanova K,Samek O,Kucera D,Benesova P,Nebesarova J

更新日期：2016-05-01 00:00:00

abstract：:The marine foodborne enteropathogen, Vibrio parahaemolyticus, has four putative catalase genes. Function of the katG-homologous genes, katG1(VPA0768) and katG2(VPA0453), was examined using gene deletion mutants, and compared with those of the katE-homologous genes, katE1(VPA1418) and katE2(VPA0305). Bacterial growth o...

journal_title：FEMS microbiology letters

authors： Yu SC,Fen SY,Chien CL,Wong HC

更新日期：2016-03-01 00:00:00

abstract：:Eighty-six strains of S. thermophilus were examined for their plasmid content. Thirteen strains were found to contain one or two plasmids ranging in size from 2.1 to 7.4 kb. DNA-DNA hybridization analysis revealed the presence of five distinct groups of DNA homology. The complete nucleotide sequence of plasmid pST1 (A...

journal_title：FEMS microbiology letters

authors： Janzen T,Kleinschmidt J,Neve H,Geis A

更新日期：1992-08-15 00:00:00

abstract：:Proteus mirabilis strain MAG1, a clinical isolate that is resistant to broad-spectrum penicillins and co-amoxiclav, produces inhibitor-resistant TEM (IRT)-21, a novel mutant of TEM beta-lactamase. This enzyme has a pI of 5.2 and is derived from the bla(TEM-1a) gene ancestor. It contains two major amino acid substituti...

journal_title：FEMS microbiology letters

authors： Mammeri H,Gilly L,Laurans G,Vedel G,Eb F,Paul G

更新日期：2001-12-18 00:00:00

abstract：:In our recent screen for soil-induced genes, the expression of andA operon (andAcAdAbAa) for anthranilate catabolism in Burkholderia multivorans ATCC 17616 was found to increase dramatically in a soil sample (Nishiyama et al., Environ Microbiol 12: 2539, 2010). The operon was preceded by andR encoding a putative trans...

journal_title：FEMS microbiology letters

authors： Nishiyama E,Ohtsubo Y,Yamamoto Y,Nagata Y,Tsuda M

更新日期：2012-05-01 00:00:00

abstract：:Fungi of the genus Fusarium are important plant pathogens and contaminants of cereal grains producing different types of mycotoxins. Enniatins are a group of mycotoxins with ionophoric properties frequently detected in North European grains. Within the Fusarium complex responsible for grain infection, Fusarium avenace...

journal_title：FEMS microbiology letters

authors： Kulik T,Jestoi M,Okorski A

更新日期：2011-01-01 00:00:00

abstract：:The influence of nutritional and physical stress on sporulation, conidial germination and vegetative biomass of Ophiocordyceps sinensis, one of the most important medicinal fungi in China and now globally, was evaluated using a two-stage culture method. All the treatments, except nutrient deprivation, enhanced conidia...

journal_title：FEMS microbiology letters

authors： Ren SY,Yao YJ

更新日期：2013-09-01 00:00:00

abstract：:High quality Fourier transform infrared (FTIR) spectra were acquired from living Micrasterias hardyi cells maintained in an IR transparent flow-through cell using a FTIR microscope coupled to a synchrotron light source. Spectral maps of living, nutrient-replete cells showed band intensities consistent with the known l...

journal_title：FEMS microbiology letters

authors： Heraud P,Wood BR,Tobin MJ,Beardall J,McNaughton D

更新日期：2005-08-15 00:00:00

abstract：:CRISPR-Cas adaptive immune systems protect bacteria from phage predation, and other foreign genetic elements such as plasmids. Significant advances have been made regarding how CRISPR-Cas systems elicit immunity; however, comparatively little is known about their regulation. To study CRISPR-Cas regulation, we describe...

journal_title：FEMS microbiology letters

authors： Hampton HG,Patterson AG,Chang JT,Taylor C,Fineran PC

更新日期：2019-06-01 00:00:00

abstract：:Clostridium difficile and Clostridium perfringens type A are infectious agents of enteric diseases. The main virulence factors of these microorganisms include toxins A and B of C. difficile (ToxA and ToxB) and enterotoxin of C. perfringens (Cpe). In this study genetic constructions have been created for the expression...

journal_title：FEMS microbiology letters

authors： Belyi IF,Varfolomeeva NA

更新日期：2003-08-29 00:00:00

abstract：:A murine monoclonal antibody, designated MA-2G9, directed against outer surface protein A (OspA) of the Lyme disease spirochete, Borrelia burgdorferi, has been produced. Antibody MA-2G9, IgG1 subclass, was purified by affinity chromatography on protein G Sepharose column and used for purification of OspA antigen from ...

journal_title：FEMS microbiology letters

authors： Abolhassani M,Pavia CS,Dunn JJ,Chiao JW

更新日期：1993-05-15 00:00:00

abstract：:An Escherichia coli mutant defective in high-affinity D-ribose transport is able to utilize the sugar as a sole carbon source, suggesting that other transport systems for D-ribose exist. In order to search for such transporters, transposon mutagenesis was carried out in an rbsB-negative strain containing ribokinase (r...

journal_title：FEMS microbiology letters

authors： Song S,Park C

更新日期：1998-06-15 00:00:00

abstract：:Lactococcus lactis MG5267 is a plasmid-free strain in which the lactose operon is integrated in the bacterial chromosome. The chromosomal lacG gene which encodes phospho-beta-galactosidase was inactivated by a double cross-over integration event. Unexpectedly, the resultant mutant was shown to retain a Lac-positive ph...

journal_title：FEMS microbiology letters

authors： Payne J,MacCormick CA,Griffin HG,Gasson MJ

更新日期：1996-02-01 00:00:00

abstract：:The use of plasmids possessing a regulatable gene coding for a site-specific recombinase together with its recognition sequences significantly facilitates genome manipulations since it allows self-excision of the portion of the genetic construct integrated into the host genome. Stable maintenance of such plasmids in E...

journal_title：FEMS microbiology letters

authors： Agaphonov MO

更新日期：2017-12-01 00:00:00

abstract：:The presence of cholesteryl glucosides and high levels of lysophospholipids are elements making the cell wall of Helicobacter pylori unique. In this study, we have investigated the relationship between lysophospholipid content and cholesteryl glucoside composition of variants of 6 clinical isolates. The samples were c...

journal_title：FEMS microbiology letters

authors： Tannaes T,Bukholm G

更新日期：2005-03-01 00:00:00

abstract：:The presence and distribution of isoprenoid glycerol dialkyl glycerol tetraethers (GDGTs), lipids that constitute the membranes of Archaea, have been investigated in a 50-cm long core from a Swedish peat bog. In the acrotelm, the periodically water saturated and thus oxic upper layer of the peat bog, only minor amount...

journal_title：FEMS microbiology letters

authors： Weijers JW,Schouten S,van der Linden M,van Geel B,Damsté JS

更新日期：2004-10-01 00:00:00

abstract：:In Azotobacter chroococcum the hydrogenase structural genes (hupSL) cover about 2.8 kb of a 15-kb region associated with hydrogen-uptake (Hup) activity. Two other genes in this region, hupD and hupE, were located 8.9 kb downstream of hupL and were shown to be essential for hydrogenase activity by insertion mutagenesis...

journal_title：FEMS microbiology letters

authors： Du L,Stejskal F,Tibelius KH

更新日期：1992-09-01 00:00:00

abstract：:The signal that triggers the expression of the ace operon and, in turn, the transition of central metabolism's architecture from acetogenic to gluconeogenic in Escherichia coli remains elusive despite extensive research both in vivo and in vitro. Here, with the aid of flux analysis together with measurements of the en...

journal_title：FEMS microbiology letters

authors： El-Mansi M,Phue JN,Shiloach J

更新日期：2021-01-08 00:00:00

abstract：:The ascomycetous black yeasts Hortaea werneckii, Phaeotheca triangularis, and Trimmatostroma salinum are halophilic fungi that inhabit hypersaline water of solar salterns. They are characterized by slow, meristematic growth and very thick, darkly pigmented cell walls. The dark pigment, generally thought to be melanin,...

journal_title：FEMS microbiology letters

authors： Kogej T,Wheeler MH,Lanisnik Rizner T,Gunde-Cimerman N

更新日期：2004-03-19 00:00:00