Abstract:
:The application of series of high intensity electric pulses to a yeast suspension provoked a considerable release of some cytoplasmic proteins, glutathione reductase, 3-phosphoglycerate kinase and alcohol dehydrogenase. A maximal yield was achieved 3-8 h after pulsation. The electro-induced protein efflux was accelerated by pretreatment with the reducing agent dithiothreitol and showed a strong dependence on the growth phase and the presence of monovalent ions in the post-pulse incubation medium. The results obtained for two strains of Saccharomyces cerevisiae, PV3 (diploid) and Y47 (wild haploid), showed that electropulsation can be used for the effective extraction of cytoplasmic proteins with a preserved functional activity.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Ganeva V,Galutzov Bdoi
10.1111/j.1574-6968.1999.tb13580.xkeywords:
subject
Has Abstractpub_date
1999-05-15 00:00:00pages
279-84issue
2eissn
0378-1097issn
1574-6968pii
S0378-1097(99)00158-5journal_volume
174pub_type
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