Evidence for importance of the Staphylococcus hyicus lipase pro-peptide in lipase secretion, stability and activity.

Abstract:

:To investigate the function of the pro-peptide (PP) region of the Staphylococcus hyicus exolipase, restriction sites were created in the lipase gene to facilitate the construction of deletions in this region. Lipase gene expression was carried out in Staphylococcus carnosus. In the presence of the entire PP region, the 86-kDa pro-lipase was efficiently exported, had high lipolytic activity, and hardly any degradation products were seen in Western blot analysis. In addition to the 86-kDa pro-lipase, the membrane fraction contained a 106-kDa immunoreactive form. If the PP was completely or partially deleted, signal peptide processing, lipase secretion, lipase activity and/or lipase stability were impaired. The results obtained with lipase PP deletion mutants indicate that the PP region may have two functional domains. The N-terminal region of the lipase PP appears to be more important for lipase activity and the C-terminal portion for lipase secretion and proteolytic stability. In the presence of only the C-terminal part of the PP lipase, secretion was hardly affected. However, the activity of the extracellular lipase was markedly reduced. If only a small portion of the C-terminal part of the PP was present, lipase secretion was again markedly reduced and no lipase activity was detectable. In the presence of the N-terminal half of the PP region, lipase secretion was affected to a lesser extent. However, the resulting 60-kDa form, which showed comparably good specific lipase activity, suffered severe proteolytic degradation.

journal_name

FEMS Microbiol Lett

authors

Demleitner G,Götz F

doi

10.1111/j.1574-6968.1994.tb07098.x

subject

Has Abstract

pub_date

1994-08-15 00:00:00

pages

189-97

issue

2

eissn

0378-1097

issn

1574-6968

pii

0378-1097(94)90126-0

journal_volume

121

pub_type

杂志文章
  • Pivotal role of anthranilate dioxygenase genes in the adaptation of Burkholderia multivorans ATCC 17616 in soil.

    abstract::In our recent screen for soil-induced genes, the expression of andA operon (andAcAdAbAa) for anthranilate catabolism in Burkholderia multivorans ATCC 17616 was found to increase dramatically in a soil sample (Nishiyama et al., Environ Microbiol 12: 2539, 2010). The operon was preceded by andR encoding a putative trans...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2012.02532.x

    authors: Nishiyama E,Ohtsubo Y,Yamamoto Y,Nagata Y,Tsuda M

    更新日期:2012-05-01 00:00:00

  • Salicylate uniquely induces a 27-kDa protein in tubercle bacillus.

    abstract::Salicylate was found to uniquely induce a 27-kDa protein in Mycobacterium tuberculosis complex organisms but not in Mycobacterium smegmatis or Escherichia coli. The structural analogue antitubercular para-amino-salicylate also induced the 27-kDa protein but to a somewhat lower level than salicylate. Other structural a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10843.x

    authors: Sun Z,Cheng SJ,Zhang H,Zhang Y

    更新日期:2001-09-25 00:00:00

  • Genome-wide identification, characterization and expression analysis of lineage-specific genes within Hanseniaspora yeasts.

    abstract::Lineage-specific genes (LSGs) are defined as genes with sequences that are not significantly similar to those in any other lineage. LSGs have been proposed, and sometimes shown, to have significant effects in the evolution of biological function. In this study, two sets of Hanseniaspora spp. LSGs were identified by co...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnaa077

    authors: Chen K,Tian Z,Chen P,He H,Jiang F,Long CA

    更新日期:2020-06-01 00:00:00

  • Hydrogenase genes are uncommon and highly conserved in Rhizobium leguminosarum bv. viciae.

    abstract::A screening for hydrogen uptake (hup) genes in Rhizobium leguminosarum bv. viciae isolates from different locations within Spain identified no Hup+ strains, confirming the scarcity of the Hup trait in R. leguminosarum. However, five new Hup+ strains were isolated from Ni-rich soils from Italy and Germany. The hup gene...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.09.022

    authors: Fernández D,Toffanin A,Palacios JM,Ruiz-Argüeso T,Imperial J

    更新日期:2005-12-01 00:00:00

  • Experimental evidence of an acetate transporter protein and characterization of acetate activation in aceticlastic methanogenesis of Methanosarcina mazei.

    abstract::Aceticlastic methanogens metabolize acetate to methane and carbon dioxide. The central metabolism and the electron transport chains of these organisms have already been investigated. However, no particular attention has been paid to the mechanism by which acetate enters the archaeal cell. In our study we investigated ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12550

    authors: Welte C,Kröninger L,Deppenmeier U

    更新日期:2014-10-01 00:00:00

  • Is the fur gene of Rhizobium leguminosarum essential?

    abstract::Using primers corresponding to conserved regions of the bacterial regulatory gene fur, a homologue of this gene from the genome of Rhizobium leguminosarum biovar viciae, the nitrogen-fixing symbiont of peas, was isolated and sequenced. The fur gene is normally expressed constitutively, independent of the presence of F...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/s0378-1097(98)00454-6

    authors: de Luca NG,Wexler M,Pereira MJ,Yeoman KH,Johnston AW

    更新日期:1998-11-15 00:00:00

  • Characterization of a periplasmic peptidyl-prolyl cis-trans isomerase in Erwinia chrysanthemi.

    abstract::The main determinant of the plant pathogen Erwinia chrysanthemi virulence is the production of extracellular enzymes, mainly pectate lyases. Adjacent to a pectate lyase encoding locus, we identified the gene rotA supposed to encode a folding catalyst. Overproduction of the protein and assay of activity using a synthet...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb12753.x

    authors: Pissavin C,Hugouvieux-Cotte-Pattat N

    更新日期:1997-12-01 00:00:00

  • Sigma(s)-dependent regulation of yehZYXW, which encodes a putative osmoprotectant ABC transporter of Escherichia coli.

    abstract::The Escherichia coli yehZYXW operon encodes a putative osmoprotectant uptake system of the ABC transporter family. yehZ is identical to osmF, an osmotically inducible gene identified previously. Construction and analysis of a yehZ-lacZ transcriptional fusion demonstrated that yehZ is inducible not only by osmolarity, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.05.046

    authors: Checroun C,Gutierrez C

    更新日期:2004-07-15 00:00:00

  • ATP/ADP alteration as a sign of the oxidative stress development in Escherichia coli cells under antibiotic treatment.

    abstract::The extensively discussed idea of oxidative stress development under antibiotic treatment was confirmed using an antioxidant gene expression (soxRS-, oxyR-regulon) approach, including microaerobic cultivation conditions. The killing action of antibiotics and their ability to cause peroxide oxidative stress in Escheric...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12405

    authors: Akhova AV,Tkachenko AG

    更新日期:2014-04-01 00:00:00

  • Altered gene expression in the transition phase by disruption of a Na+/H+ antiporter gene (shaA) in Bacillus subtilis.

    abstract::The shaA gene (sodium-hydrogen antiporter gene A, identical to mrpA) is largely responsible for Na+ extrusion in Bacillus subtilis. The disruption of shaA combined with a low concentration of NaCl completely abolishes sporulation but allows normal growth. To investigate the role of shaA and shaA-mediated sodium ion ho...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(04)00037-0

    authors: Kosono S,Asai K,Sadaie Y,Kudo T

    更新日期:2004-03-12 00:00:00

  • The phosphotransferase system gene ptsI in the endophytic bacterium Bacillus cereus is required for biofilm formation, colonization, and biocontrol against wheat sharp eyespot.

    abstract::Natural resistance of wheat plants to wheat sharp eyespot is inadequate, and new strategies for controlling the disease are required. Biological control is an alternative and attractive way of reducing the use of chemicals in agriculture. In this study, we investigated the biocontrol properties of endophytic bacterium...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12438

    authors: Xu YB,Chen M,Zhang Y,Wang M,Wang Y,Huang QB,Wang X,Wang G

    更新日期:2014-05-01 00:00:00

  • Identification of the glycosyl transferase required for synthesis of the principal glycolipid characteristic of heterocysts of Anabaena sp. strain PCC 7120.

    abstract::Nitrogenase is oxygen-labile. Cyanobacterial heterocysts can fix N(2) in an oxic milieu because their interior is micro-oxic, for which the glycolipid layer of the heterocyst envelope is required. ORF all5341 of the Anabaena sp. genome predicts a glycosyl transferase. An insertional mutant of all5341 synthesized only ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00512.x

    authors: Awai K,Wolk CP

    更新日期:2007-01-01 00:00:00

  • Probiotic Enterococcus faecalis AG5 effectively assimilates cholesterol and produces fatty acids including propionate.

    abstract::Probiotics are known for their health-promoting abilities through the production of various metabolites, and also benefits are membrane associated. Probiotic Enterococcus faecalis AG5 has been previously assessed for its probiotic properties and this study further strengthens that claim. Strain AG5 was inoculated with...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz039

    authors: Mishra AK,Kumar SS,Ghosh AR

    更新日期:2019-02-01 00:00:00

  • Protein expression by Streptococcus uberis in co-culture with bovine mammary epithelial cells.

    abstract::Three strains of Streptococcus uberis isolated from dairy cows with mastitis were co-cultured with a bovine mammary epithelial cell line (MAC-T) in Dulbecco's modified Eagle's medium without fetal bovine serum. Protein profiles from culture supernatants and bacterial pellets among different treatments were compared by...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13896.x

    authors: Fang W,Luther DA,Oliver SP

    更新日期:1998-09-15 00:00:00

  • Cloning and sequencing of a cyclodextrin glycosyltransferase gene from Brevibacillus brevis CD162 and its expression in Escherichia coli.

    abstract::A cyclodextrin glycosyltransferase (CGTase) gene of Brevibacillus brevis CD162 was cloned into Escherichia coli using pUC19 as a vector. Determination of the nucleotide sequence showed the presence of an open reading frame of 2079 bp encoding a polypeptide of 693 amino acid residues, composed of a 20-amino acid signal...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13117.x

    authors: Kim MH,Sohn CB,Oh TK

    更新日期:1998-07-15 00:00:00

  • In vivo proteome analysis of Xanthomonas campestris pv. campestris in the interaction with the host plant Brassica oleracea.

    abstract::The genus Xanthomonas is composed of several species that cause severe crop losses around the world. In Latin America, one of the most relevant species is Xanthomonas campestris pv. campestris, which is responsible for black rot in cruciferous plants. This pathogen causes yield losses in several cultures, including ca...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01090.x

    authors: Andrade AE,Silva LP,Pereira JL,Noronha EF,Reis FB Jr,Bloch C Jr,dos Santos MF,Domont GB,Franco OL,Mehta A

    更新日期:2008-04-01 00:00:00

  • The impact factor: implications for research policy, editorial rules and scholarly reputation.

    abstract::The assessment of scientific research is essentially based on several metric parameters, the so-termed Impact Factor perhaps being the predominant one. Despite well-founded criticisms and the wide opposition of reputed scientists, this procedure has become a tool of scientific policy, and is applied in editorial proce...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz132

    authors: Argüelles JC,Argüelles-Prieto R

    更新日期:2019-06-01 00:00:00

  • Cloning and sequencing of a dextranase-encoding cDNA from Penicillium minioluteum.

    abstract::A cDNA from Penicillium minioluteum HI-4 encoding a dextranase (1,6-alpha-glucan hydrolase, EC 3.2.1.11) was isolated and characterized. cDNA clones corresponding to genes expressed in dextran-induced cultures were identified by differential hybridization. Southern hybridization and restriction mapping analysis of sel...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08477.x

    authors: Garcia B,Margolles E,Roca H,Mateu D,Raices M,Gonzales ME,Herrera L,Delgado J

    更新日期:1996-10-01 00:00:00

  • The pWW0 plasmid imposes a stochastic expression regime to the chromosomal ortho pathway for benzoate metabolism in Pseudomonas putida.

    abstract::Environmental plasmids often expand the metabolic repertoire of bacteria that carry them, but they also interfere with the biochemical and genetic network of the host. The pWW0 plasmid born by Pseudomonas putida mt-2 encodes the TOL pathway for degradation of toluene/m-xylene through production of intermediate compoun...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12400

    authors: Silva-Rocha R,de Lorenzo V

    更新日期:2014-07-01 00:00:00

  • Suppression of an Escherichia coli secA(ts) mutant by a gene cloned from Staphylococcus carnosus.

    abstract::Escherichia coli mutant MM52 (secA(ts)) was transformed with a cosmid library from Staphylococcus carnosus, and a recombinant cosmid (pBO23) allowing growth at the non-permissive temperature (42 degrees C) was isolated. pBO23 also restored the growth defects of E. coli mutants IQ85 (secY(ts)) and IT41 (lep(ts)). Nucle...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(91)90118-t

    authors: Overhoff-Freundlieb B,Freudl R

    更新日期:1991-11-15 00:00:00

  • Biogenic amine-forming microbial communities in cheese.

    abstract::The aim of this study was to screen two cheese starter cultures and cheese-borne microbial communities with the potential to produce biogenic amines in cheese during ripening. Bacteria of the genera Enterococcus and Lactobacillus and coliform bacteria were isolated from Dutch-type semi-hard cheese at the beginning of ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00922.x

    authors: Burdychova R,Komprda T

    更新日期:2007-11-01 00:00:00

  • Low-moisture food matrices as probiotic carriers.

    abstract::To exert a beneficial effect on the host, adequate doses of probiotics must be administered and maintaining their viability until consumption is thus essential. Dehydrated probiotics exhibit enhanced long-term viability and can be incorporated into low-moisture food matrices, which also possess high stability at refri...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fnz006

    authors: Marcial-Coba MS,Knøchel S,Nielsen DS

    更新日期:2019-01-01 00:00:00

  • GeneChip expression analysis of the VqsR regulon of Pseudomonas aeruginosa TB.

    abstract::Two interlinked quorum sensing circuits, las and rhl, which control pathogenesis of Pseudomonas aeruginosa are further modulated by numerous regulators, including VqsR (virulence and quorum sensing regulator). High-density oligonucleotide microarrays were used to compare the global expression profile of a wild-type an...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.11.020

    authors: Juhas M,Wiehlmann L,Salunkhe P,Lauber J,Buer J,Tümmler B

    更新日期:2005-01-15 00:00:00

  • DNA probes for identification of the ectomycorrhizal fungus Tuber magnatum Pico.

    abstract::The random amplified polymorphic DNA (RAPD) technique was used to develop DNA probes for the identification of ectomycorrhizal fungi belonging to the genus Tuber. RAPD fingerprinting revealed a high degree of interspecific variability and a low degree of intraspecific variability. One band (approximately 1.5 kb), cons...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06581.x

    authors: Lanfranco L,Wyss P,Marzachí C,Bonfante P

    更新日期:1993-12-15 00:00:00

  • Microbiome characterization of poultry products based on the poultry part and production label.

    abstract::Poultry is one of the most consumed meats worldwide and there are many different types of poultry products available on the market for consumers. Many poultry producers provide a variety of labeled meats based on rearing system such as organic, free-range and antibiotic-free. However, these labels often can be mislead...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz092

    authors: Lee SI,Choi J,Daeschel DZ,Park SH

    更新日期:2019-05-01 00:00:00

  • Functional analysis of Borrelia burgdorferi uvrA in DNA damage protection.

    abstract::Bacterial pathogens face constant challenges from DNA-damaging agents generated by host phagocytes. Although Borrelia burgdorferi appears to have much fewer DNA repair enzymes than pathogens with larger genomes, it does contain homologues of uvrA and uvrB (subunits A and B of excinuclease ABC). As a first step to expl...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02226.x

    authors: Sambir M,Ivanova LB,Bryksin AV,Godfrey HP,Cabello FC

    更新日期:2011-04-01 00:00:00

  • Colony immunoblot assay for the detection of hemolysin BL enterotoxin producing Bacillus cereus.

    abstract::Bacillus cereus strains involved in food poisoning cases of the diarrheal type may produce two different enterotoxin complexes. To facilitate the identification of hemolysin BL-enterotoxin complex (HBL) and/or the nonhemolytic enterotoxin (NHE) producing colonies a colony immunoblot procedure was developed, which allo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.07.024

    authors: Moravek M,Wegscheider M,Schulz A,Dietrich R,Bürk C,Märtlbauer E

    更新日期:2004-09-01 00:00:00

  • Phospholipids of Clostridium perfringens: a reexamination.

    abstract::We have identified phosphatidylethanolamine as one of the major phospholipids of Clostridium perfringens by two dimensional thin layer chromatography of the intact lipids and of their deacylation products and by liquid chromatography followed by mass spectrometry of the intact neutral phospholipid fraction. The princi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.01.048

    authors: Johnston NC,Baker JK,Goldfine H

    更新日期:2004-04-01 00:00:00

  • Isolation and preliminary characterisation of twenty-five temperature-sensitive mutants of mouse cytomegalovirus.

    abstract::To study the pathogenicity of mouse cytomegalovirus (MCMV) and to identify virulence determinants, we have isolated and phenotypically characterised 25 temperature-sensitive (ts) mutants. Six of these (tsm9, tsm13, tsm20, tsm22, tsm28 and tsm30) failed to replicate in mice and were avirulent. Five mutants (tsm14, tsm1...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06523.x

    authors: Akel HM,Sweet C

    更新日期:1993-11-01 00:00:00

  • Genetic engineering of Streptococcus gordonii for the simultaneous display of two heterologous proteins at the bacterial surface.

    abstract::The Gram-positive bacterium Streptococcus gordonii has been genetically engineered to allow the simultaneous expression of two heterologous proteins at the cell surface. A family of recombinant streptococci displaying two different antigens was constructed. All the strains were genetically stable and expressed both pr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11172.x

    authors: Maggi T,Spinosa M,Ricci S,Medaglini D,Pozzi G,Oggioni MR

    更新日期:2002-04-23 00:00:00