Abstract:
:Using primers corresponding to conserved regions of the bacterial regulatory gene fur, a homologue of this gene from the genome of Rhizobium leguminosarum biovar viciae, the nitrogen-fixing symbiont of peas, was isolated and sequenced. The fur gene is normally expressed constitutively, independent of the presence of Fe in the medium, but in one Rhizobium strain it was transcribed at a low level. Attempts to isolate a fur knockout mutant failed, suggesting that the gene is essential for free-living growth. In other bacteria, certain fur mutations confer manganese resistance; however, none of the manganese-resistant mutants of R. leguminosarum which we isolated was corrected by the cloned fur gene. When the cloned R. leguminosarum fur gene was introduced into a fur mutant of Escherichia coli, it caused some Fe-dependent reduction in the amount of siderophore, indicating that it can function heterologously.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
de Luca NG,Wexler M,Pereira MJ,Yeoman KH,Johnston AWdoi
10.1016/s0378-1097(98)00454-6subject
Has Abstractpub_date
1998-11-15 00:00:00pages
289-95issue
2eissn
0378-1097issn
1574-6968pii
S0378-1097(98)00454-6journal_volume
168pub_type
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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