Protein expression by Streptococcus uberis in co-culture with bovine mammary epithelial cells.

abstract：:The effect of Listeria monocytogenes infection on the cellular level of transcription factor NF-kappa B in the human epithelia-like cell line Caco-2 was investigated. Infection with L. monocytogenes or treatment with lipoteichoic acid induced the formation of three NF-kappa B-like DNA-protein complexes C1, C2, and C3,...

journal_title：FEMS microbiology letters

authors： Hauf N,Goebel W,Fiedler F,Kuhn M

更新日期：1999-09-01 00:00:00

abstract：:A total of 129 sRNA candidates were identified in Brucella abortus 2308 in our previous work, and one candidate with potential to regulate expression of hemH gene was further analyzed in this study. We found that the novel sRNA can inhibit the expression of hemH and called it BsrH (Brucella sRNA regulating HemH). The ...

journal_title：FEMS microbiology letters

authors： Peng X,Dong H,Wu Q

更新日期：2015-01-01 00:00:00

abstract：:Francisella tularensis is a highly virulent bacterial species that causes various forms of tularemia in humans. The urgency in understanding the pathogenesis of these diseases has stimulated unprecedented interest in this bacterial species over the past few years. Recent findings underscore a number of important disti...

journal_title：FEMS microbiology letters

authors： Parmely MJ,Fischer JL,Pinson DM

更新日期：2009-11-01 00:00:00

abstract：:To rapidly identify natural isolates of marine bioluminescent bacteria, we developed amplified ribosomal DNA restriction analysis (ARDRA) methods. ARDRA, which is based on the restriction patterns of 16S rRNA gene digested with five enzymes (EcoRI, DdeI, HhaI, HinfI, RsaI), clearly distinguished the 14 species of mari...

journal_title：FEMS microbiology letters

authors： Kita-Tsukamoto K,Wada M,Yao K,Kamiya A,Yoshizawa S,Uchiyama N,Kogure K

更新日期：2006-03-01 00:00:00

abstract：:The Saccharomyces sensu stricto genus contains many species that are industrially important for fermentation of wines, beers and ales. The molecular characterization of the genomes of yeasts involved in these processes reveals that the majority arose from interspecific hybridization between two and sometimes three yea...

journal_title：FEMS microbiology letters

authors： Querol A,Bond U

更新日期：2009-04-01 00:00:00

abstract：:The constitutive expression vector pHY300-F1gfp was constructed to test the function of a promoter, F1, cloned from the rice epiphyte Bacillus brevis strain DX01. The DX01 cells harboring the plasmid pHY300-F1gfp were shown to produce bright green fluorescence. The results were confirmed by Western blot analysis and f...

journal_title：FEMS microbiology letters

authors： Chen Y,Yan J,Yang M,Wang J,Shen D

更新日期：2003-12-05 00:00:00

abstract：:A species of Dechlorospirillum was isolated from an Fe(II)-oxidizing, opposing-gradient-culture enrichment using an inoculum from a circumneutral, freshwater creek that showed copious amounts of Fe(III) (hydr)oxide precipitation. In gradient cultures amended with a redox indicator to visualize the depth of oxygen pene...

journal_title：FEMS microbiology letters

authors： Picardal FW,Zaybak Z,Chakraborty A,Schieber J,Szewzyk U

更新日期：2011-06-01 00:00:00

abstract：:Saccharomyces cerevisiae was engineered for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum. The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C. ce...

journal_title：FEMS microbiology letters

authors： Hyeon JE,Yu KO,Suh DJ,Suh YW,Lee SE,Lee J,Han SO

更新日期：2010-09-01 00:00:00

abstract：:Proteus mirabilis strain MAG1, a clinical isolate that is resistant to broad-spectrum penicillins and co-amoxiclav, produces inhibitor-resistant TEM (IRT)-21, a novel mutant of TEM beta-lactamase. This enzyme has a pI of 5.2 and is derived from the bla(TEM-1a) gene ancestor. It contains two major amino acid substituti...

journal_title：FEMS microbiology letters

authors： Mammeri H,Gilly L,Laurans G,Vedel G,Eb F,Paul G

更新日期：2001-12-18 00:00:00

abstract：:Endpolygalacturonase I from Stereum purpureum has been identified as a causative substance for the silver-leaf disease in apples. It possesses a unique pro-sequence in the C-terminal region that lacks endpolygalacturonases from any other origin. In this study, we analyzed and compared enzymatic characteristics between...

journal_title：FEMS microbiology letters

authors： Hamada S,Toda K,Ogawa S,Kubota K,Miyairi K

更新日期：2015-09-01 00:00:00

abstract：:PhoP was previously shown to be important for Yersinia pestis survival in macrophage and under macrophage-induced stresses. In this work, a phoP disruptant of Y. pestis 201 was generated using the Red cloning procedure. The transcription profile of the wild-type Y. pestis was then compared with that of the phoP mutant...

journal_title：FEMS microbiology letters

authors： Zhou D,Han Y,Qin L,Chen Z,Qiu J,Song Y,Li B,Wang J,Guo Z,Du Z,Wang X,Yang R

更新日期：2005-09-01 00:00:00

abstract：:The small noncoding RNA RyhB is a regulator of iron homeostasis in Escherichia coli. During iron limitation, it downregulates the expression of a number of iron-containing proteins, including enzymes of the tricarboxylic acid cycle and the respiratory chain. Because this infers a potential for RyhB to limit energy met...

journal_title：FEMS microbiology letters

authors： Bollinger CJ,Kallio PT

更新日期：2007-10-01 00:00:00

abstract：:An isolation procedure was developed to provide within one day microcystin-LR, a cyclic heptapeptide toxin from Microcystis aeruginosa PCC 7806. After ODS (octadecylsilyl) solid phase extraction, the crude toxin fraction was chromatographed using a strong anion exchange column. The toxin was eluted with 0.02 M ammoniu...

journal_title：FEMS microbiology letters

authors： Martin C,Sivonen K,Matern U,Dierstein R,Weckesser J

更新日期：1990-03-01 00:00:00

abstract：:Streptococcus equi ssp. equi is the causative agent of strangles, a highly contagious and serious disease in the upper respiratory tract of horses. The present study describes the characterization of IdeE, a homolog of the secreted IgG-specific protease IdeS/Mac of Streptococcus pyogenes. The activity of IdeE is compa...

journal_title：FEMS microbiology letters

authors： Lannergård J,Guss B

更新日期：2006-09-01 00:00:00

abstract：:Two actinomycete strains, CHR3 and CHR28, were isolated from metal-contaminated sediments from Baltimore Inner Harbor. The isolates were classified as Streptomyces spp. Agar diffusion assays showed both isolates to be resistant to mercuric chloride and phenylmercuric acetate. Hybridization experiments indicated that g...

journal_title：FEMS microbiology letters

authors： Ravel J,Amoroso MJ,Colwell RR,Hill RT

更新日期：1998-05-01 00:00:00

abstract：:Bjerkandera adusta produces many chlorometabolites including chlorinated anisyl metabolites (CAMs) and 1-arylpropane-1,2-diols (1, 2, 3, 4) as idiophasic metabolic products of L-phenylalanine. These diols are stereoselectively biosynthesized from a C7-unit (benzylic, from L-phenylalanine) and a C2-unit, of unknown ori...

journal_title：FEMS microbiology letters

authors： Silk PJ,Macaulay JB

更新日期：2003-11-07 00:00:00

abstract：:NadR is the repressor protein that controls the expression of genes for NAD synthesis. It is also believed to be involved in nucleotide transport. Point mutations conferring different phenotypes were localized to six different regions within the nadR gene. That mutations affecting repression and transport all mapped w...

journal_title：FEMS microbiology letters

authors： Foster JW,Penfound T

更新日期：1993-09-01 00:00:00

abstract：:The adhesion-promoting proteins (APP) (molecular mass approx. 300 kDa), which promote adhesion of Streptococcus mutans JBP (serotype c) to hydroxyapatite, were isolated from human submandibular-sublingual (SMSL) saliva by gel filtration on a Trisacryl GP2000 M column. The effects of hexoses, pentoses, methyl-pentoses,...

journal_title：FEMS microbiology letters

authors： Kishimoto E,Hay DI,Gibbons RJ

更新日期：1991-12-15 00:00:00

abstract：:Microcystins produced by cyanobacteria are potent and specific hepatotoxins; however, the mechanisms of microcystin-induced hepatotoxicity have not been fully elucidated. The induction of free radical formation and mitochondrial alterations are two major events found in microcystin-treated cultured rat hepatocytes. Th...

journal_title：FEMS microbiology letters

authors： Ding WX,Nam Ong C

更新日期：2003-03-14 00:00:00

abstract：:beta-Glucosidase in Aspergillus nidulans was found to be both intracellular and extracellular. The intracellular beta-glucosidase was synthesized after the exhaustion of carbon source in the medium. The extracellular enzyme appeared with autolysis of the mycelium. Biosynthesis of beta-glucosidase was not induced by va...

journal_title：FEMS microbiology letters

authors： Lee J,Kwon KS,Hah YC

更新日期：1996-01-01 00:00:00

abstract：:The purpose of this study was to generate highly specific serological reagents for the quantitative identification of Actinomyces naeslundii in clinical samples, in particular dental plaque. Balb/c mice were immunized with pasteurized human A. naeslundii strains representing different genospecies and serotypes. Ten hy...

journal_title：FEMS microbiology letters

authors： Thurnheer T,Guggenheim B,Gmür R

更新日期：1997-05-15 00:00:00

abstract：:The lipid modification and processing of truncated mutants of the colicin A lysis protein were observed after overproduction by Escherichia coli bacteria, but at a rate far slower than that of the wild-type. The unmodified precursor form of the mutants was stable over hour(s). The truncated mutants provoked lethality,...

journal_title：FEMS microbiology letters

authors： Cavard D

更新日期：1994-04-01 00:00:00

abstract：:An Escherichia coli mutant defective in high-affinity D-ribose transport is able to utilize the sugar as a sole carbon source, suggesting that other transport systems for D-ribose exist. In order to search for such transporters, transposon mutagenesis was carried out in an rbsB-negative strain containing ribokinase (r...

journal_title：FEMS microbiology letters

authors： Song S,Park C

更新日期：1998-06-15 00:00:00

abstract：:Acinetobacter baumannii is an important nosocomial pathogen that displays high antibiotic resistance. It causes a variety of infections including pneumonias and sepsis which may result in disseminated intravascular coagulation. In this work, we identify and characterize a novel secreted, zinc-dependent, metallo-endope...

journal_title：FEMS microbiology letters

authors： Tilley D,Law R,Warren S,Samis JA,Kumar A

更新日期：2014-07-01 00:00:00

abstract：:The physiology of the response in the methanotrophic bacterium Methylococcus capsulatus Bath towards thermal and solvent stress was studied. A systematic investigation of the toxic effects of organic compounds (chlorinated phenols and alkanols) on the growth of this bacterium was carried out. The sensitivity to the te...

journal_title：FEMS microbiology letters

authors： Löffler C,Eberlein C,Mäusezahl I,Kappelmeyer U,Heipieper HJ

更新日期：2010-07-01 00:00:00

abstract：:Two monoclonal antibodies (G6 and 7B), generated against a 63-kDa stress protein (GSP63) from Neisseria gonorrhoeae strain VP1, were used to investigate the antigenic heterogeneity of GSP63 among the Neisseriaceae and its antigenic relationship with the Hsp60 heat-shock protein family. Immunoblotting experiments demon...

journal_title：FEMS microbiology letters

authors： Pannekoek Y,Dankert J,van Putten JP

更新日期：1992-11-15 00:00:00

abstract：:We report on the specificity of a monoclonal antibody which reacts with autoclaved extracts of four species of enterococci but does not react to the same extent with similar extracts from two non-enterococcal group D streptococci. The monoclonal antibody also reacts specifically with purified lipoteichoic acid from St...

journal_title：FEMS microbiology letters

authors： Hammonds SJ,Easterbrook TJ,McIllmurray MB

更新日期：1991-07-15 00:00:00

abstract：:A gene expression system for both Bacillus subtilis and Escherichia coli was developed. The expression vector, pHASH102, produces any combination of promoter and open reading frame to be expressed based on the T-extended cloning method. Because the pHASH series vectors are designed to shuttle between the genome and a ...

journal_title：FEMS microbiology letters

authors： Ohashi Y,Ohshima H,Tsuge K,Itaya M

更新日期：2003-04-11 00:00:00

abstract：:Cronobacter sakazakii is associated with severe and often fatal cases of infant meningitis and necrotizing enterocolitis. The form of meningitis differs from that due to Neisseria meningitidis and Streptococcus spp., in that it is highly invasive and destructive towards human brain cells. However, there is relatively ...

journal_title：FEMS microbiology letters

authors： Alzahrani H,Winter J,Boocock D,De Girolamo L,Forsythe SJ

更新日期：2015-06-01 00:00:00

abstract：:Small colony variants (SCVs) of Pseudomonas aeruginosa NCTC 6750 (WT) were repeatedly isolated in an in vitro kinetic model after exposure to gentamicin (GM). There were minor differences biochemically and in phage and serotyping between the wild type (WT) strain and SCVs. Changes in outer membrane protein profiles we...

journal_title：FEMS microbiology letters

authors： Langford PR,Anwar H,Gonda I,Brown MR

更新日期：1989-10-01 00:00:00