Abstract:
:Listeria monocytogenes is a facultative intracellular pathogen responsible for both invasive and non-invasive food-borne illness in animals and humans. In this study, macrorestriction analysis following pulsed-field gel electrophoresis was used to show that Listeria monocytogenes serovar 1/2a strain EGD has a single chromosome containing eight NotI fragments of 1100, 850, 365, 320, 275, 40, 30 and 20 kb in size and 11 AscI fragments of 860, 470, 410, 360, 320, 250, 110, 80, 50, 30 and 20 kb. The total genome therefore comprises 3000 +/- 50 kb. The creation of a physical and genetic map of the Listeria genome was achieved by generating NotI linking clones and their use in subsequent hybridisation analysis. Using isogenic mutants harbouring additional artificial NotI restriction sites, we were able to precisely map the positions of all currently known virulence genes on the chromosome.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
von Both U,Otten S,Darbouche A,Domann E,Chakraborty Tdoi
10.1111/j.1574-6968.1999.tb13632.xkeywords:
subject
Has Abstractpub_date
1999-06-15 00:00:00pages
281-9issue
2eissn
0378-1097issn
1574-6968pii
S0378-1097(99)00207-4journal_volume
175pub_type
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