Abstract:
:Cell wall LPS of Escherichia coli are organized as particles which are visible in the electron microscope, after treatment of the wall with alkali. We now describe alkali treated walls of three E. coli strains with differences in susceptibility to the T4 phage infection. Strain CR63, a usual host for the T4 phage, shows the LPS particles on the murein layer. These particles are absent in alkali treated cell walls of the strain W. Walls of this strain are broken during T4 infection and phages can be seen bearing pieces of membrane attached to their long as well as their short tail fibers. Strain AS19 which is hypersensitive to the lysis from without caused by T4 shows murein layers with no LPS particles on their surface, and networks of LPS particles with bacterial shape. This suggested that LPS are organized in a network of particles which may serve as the skeleton of the cell wall.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Zorzopulos J,de Long S,Chapman V,Kozloff LMdoi
10.1016/0378-1097(89)90163-8subject
Has Abstractpub_date
1989-10-01 00:00:00pages
23-6issue
1-2eissn
0378-1097issn
1574-6968journal_volume
52pub_type
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