Molecular cloning and DNA sequence of dniR, a gene affecting anaerobic expression of the Escherichia coli hexaheme nitrite reductase.

abstract：:In Azotobacter chroococcum the hydrogenase structural genes (hupSL) cover about 2.8 kb of a 15-kb region associated with hydrogen-uptake (Hup) activity. Two other genes in this region, hupD and hupE, were located 8.9 kb downstream of hupL and were shown to be essential for hydrogenase activity by insertion mutagenesis...

journal_title：FEMS microbiology letters

authors： Du L,Stejskal F,Tibelius KH

更新日期：1992-09-01 00:00:00

abstract：:A CO-reactive hemoprotein was purified from the mitochondrial membrane fraction of Tetrahymena pyriformis. It showed absorption peaks at 615 and 455 nm in the reduced form and an alpha peak at 565 nm in the pyridine ferrohemochrome spectrum. Although the spectral properties were apparently similar to those of 'cytochr...

journal_title：FEMS microbiology letters

authors： Inokuchi A,Fukumori Y

更新日期：1993-08-15 00:00:00

abstract：:Coxiella is a genus of obligate intracellular bacteria engaged in a variety of interactions with eukaryotes. The type species, Coxiella burnetii, infects several vertebrate species, including humans, and is the causative agent of Q fever. Multiple copies of a specific transposable element, the insertion sequence IS111...

journal_title：FEMS microbiology letters

authors： Duron O

更新日期：2015-09-01 00:00:00

abstract：:Tn10 transposon mutagenesis of Escherichia coli producing K99 fimbriae was carried out to identify host factors involved in regulation of biosynthesis of fimbriae. Two chromosomal mutants were obtained that showed a strongly reduced cell surface expression of K99 fimbriae upon colony blotting and ELISA. Analysis by in...

journal_title：FEMS microbiology letters

authors： Pilipcinec E,Huisman TT,Willemsen PT,Appelmelk BJ,de Graaf FK,Oudega B

更新日期：1994-10-15 00:00:00

abstract：:Triclosan is the active ingredient in a multitude of health care and consumer products with germicidal properties, which have flooded the market in recent years in response to the public's fear of communicable bacteria. Although originally thought to kill bacteria by attacking multiple cellular targets, triclosan was ...

journal_title：FEMS microbiology letters

authors： Schweizer HP

更新日期：2001-08-07 00:00:00

abstract：:The glnAntrBC operon of Proteus vulgaris was cloned and heterologously expressed in Escherichia coli. The nucleotide sequence was determined. An open reading frame of 1407 bp was identified as the glnA gene and the deduced amino acid sequence showed 82% identity with the E. coli glutamine synthetase protein. Heterolog...

journal_title：FEMS microbiology letters

authors： Steglitz-Mörsdorf U,Mörsdorf G,Kaltwasser H

更新日期：1993-01-15 00:00:00

abstract：:Rhizobium leguminosarum bv. trifolii rosR gene encodes a transcriptional regulator involved in the positive regulation of exopolysaccharide synthesis. Transcription of rosR is directed by two promoters, distal P1 and proximal P2, of different strengths. We demonstrated that rosR P1 functions as the main promoter and, ...

journal_title：FEMS microbiology letters

authors： Janczarek M,Skorupska A

更新日期：2009-02-01 00:00:00

abstract：:Trehalase was studied in Schizosaccharomyces pombe cells growing vegetatively on minimal medium and in sporulating cultures. Acid trehalase activity, measured at pH 4.2, was absent in vegetative cells and occurred only in asci, indicating that this activity represented the sporulation-specific trehalase reported previ...

journal_title：FEMS microbiology letters

authors： De Virgilio C,Müller J,Boller T,Wiemken A

更新日期：1991-11-01 00:00:00

abstract：:Segmented filamentous bacteria (SFB) are inhabitants of the small intestine of various animals, where they can be detected microscopically due to their specific morphology and intimate association with the intestinal epithelium. SFB colonize the distal part of the small intestine in a host-specific manner and affects ...

journal_title：FEMS microbiology letters

authors： Jonsson H

更新日期：2013-05-01 00:00:00

abstract：:Both form I and II ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) genes were detected in Thiobacillus intermedius by heterologous hybridization using specific probes from Anacystis nidulans and Rhodobacter sphaeroides, respectively. However, only the previously reported form I enzyme could be demonstrated i...

journal_title：FEMS microbiology letters

authors： Stoner MT,Shively JM

更新日期：1993-03-01 00:00:00

abstract：:Atypical enteropathogenic Escherichia coli (EPEC) comprise an important group of paediatric pathogens. Atypical EPEC have reservoirs in farm and domestic animals where they can be either commensal or pathogenic; serogroup O26 is dominant in humans and animals. Central to intestinal colonization by EPEC is the transloc...

journal_title：FEMS microbiology letters

authors： Ooka T,Vieira MA,Ogura Y,Beutin L,La Ragione R,van Diemen PM,Stevens MP,Aktan I,Cawthraw S,Best A,Hernandes RT,Krause G,Gomes TA,Hayashi T,Frankel G

更新日期：2007-06-01 00:00:00

abstract：:The symbiotic plasmid (pSym) DNA present in bacteroids of strain RCR1001 of Rhizobium leguminosarum biovar viceae has been compared qualitatively and quantitatively with that present in free living bacteria by hybridization experiments with appropriate probes. A decrease in the relative amount of pSym DNA was observed...

journal_title：FEMS microbiology letters

authors： Vierny C,Iaccarino M

更新日期：1989-07-01 00:00:00

abstract：:In addition to core Cas proteins, CRISPR-Cas loci often encode ancillary proteins that modulate the activity of the respective effectors in interference. Subtype VI-B1 CRISPR-Cas systems encode the Csx27 protein that down-regulates the activity of Cas13b when the type VI-B locus is expressed in Escherichia coli. We sh...

journal_title：FEMS microbiology letters

authors： Makarova KS,Gao L,Zhang F,Koonin EV

更新日期：2019-04-01 00:00:00

abstract：:We have developed a novel type of a positive screen for the discovery of antibacterial compounds that target the Escherichia coli replication initiator protein DnaA. DnaA is an essential replication protein, conserved in (almost) all bacteria--including all human pathogens--and no existing antibiotics target the main ...

journal_title：FEMS microbiology letters

authors： Fossum S,De Pascale G,Weigel C,Messer W,Donadio S,Skarstad K

更新日期：2008-04-01 00:00:00

abstract：:Eighty-six strains of S. thermophilus were examined for their plasmid content. Thirteen strains were found to contain one or two plasmids ranging in size from 2.1 to 7.4 kb. DNA-DNA hybridization analysis revealed the presence of five distinct groups of DNA homology. The complete nucleotide sequence of plasmid pST1 (A...

journal_title：FEMS microbiology letters

authors： Janzen T,Kleinschmidt J,Neve H,Geis A

更新日期：1992-08-15 00:00:00

abstract：:The purpose of this study was to investigate the feasibility of cultivating the biotechnologically important bacterium Streptomyces griseus in single-species and mixed-species biofilms using a tubular biofilm reactor (TBR). Streptomyces griseus biofilm development was found to be cyclical, starting with the initial ad...

journal_title：FEMS microbiology letters

authors： Winn M,Casey E,Habimana O,Murphy CD

更新日期：2014-03-01 00:00:00

abstract：:The SbmA protein is involved in the transport of MccB17-, MccJ25-, bleomycin- and proline-rich peptides into the Escherichia coli cytoplasm. sbmA gene homologues were found in a variety of bacteria. However, the physiological role of this protein still remains unknown. Previously, we found that a combination of sbmA a...

journal_title：FEMS microbiology letters

authors： Corbalán NS,Adler C,de Cristóbal RE,Pomares MF,Delgado MA,Vincent PA

更新日期：2010-10-01 00:00:00

abstract：:Mycobacterium smegmatis was used to study the relationship between DNA repair processes involving RecA and nonhomologous end joining (NHEJ). The effect of gene deletions in recA and/or in two genes involved in NHEJ (ku and ligD) was tested on the ability of bacteria to join breaks in plasmids transformed into them and...

journal_title：FEMS microbiology letters

authors： Korycka-Machala M,Brzostek A,Rozalska S,Rumijowska-Galewicz A,Dziedzic R,Bowater R,Dziadek J

更新日期：2006-05-01 00:00:00

abstract：:We tested the possibility of nonconjugative lateral DNA transfer in a colony biofilm of mixed Escherichia coli strains. By simply coculturing a plasmid-free F(-) strain and another F(-) strain harboring a nonconjugative plasmid in a colony biofilm on antibiotic-free agar media, transformed cells were produced within 2...

journal_title：FEMS microbiology letters

authors： Maeda S,Ito M,Ando T,Ishimoto Y,Fujisawa Y,Takahashi H,Matsuda A,Sawamura A,Kato S

更新日期：2006-02-01 00:00:00

abstract：:An 18-kDa protein that occurs in Vibrio cholerae has been described as an in vivo and low-iron regulated outer membrane antigen. Monoclonal antibodies which recognized this antigen were protective as passive vaccines in the infant rabbit model of cholera disease. In this study, those monoclonal antibodies were used in...

journal_title：FEMS microbiology letters

authors： Sciortino CV Jr

更新日期：1993-07-15 00:00:00

abstract：:A DNA segment located immediately upstream of the eaeA gene of enterohemorrhagic Escherichia coli O157:H7 strain HA1 was cloned and sequenced. This segment contained an open reading frame encoding a predicted protein of 156 amino acids. A database search identified similar open reading frames upstream of the eaeA gene...

journal_title：FEMS microbiology letters

authors： Zhao S,Mitchell SE,Meng J,Doyle MP,Kresovich S

更新日期：1995-11-01 00:00:00

abstract：:Amanita exitialis Zhu L. Yang and T.H. Li is a lethal mushroom species recently isolated in Guangdong Province, China. In this report, a pure culture of this species was obtained for the first time. To confirm the identity of the pure culture, the internal transcribed spacer regions of the nuclear ribosomal DNA of the...

journal_title：FEMS microbiology letters

authors： Zhang P,Chen Z,Hu J,Wei B,Zhang Z,Hu W

更新日期：2005-11-15 00:00:00

abstract：:Strains of the filamentous gliding bacterium Vitreoscilla, LB13 and C1, are shown to be highly sensitive to UV-A (320-400 nm), with an LD50 of less than 20 kJ m(-2). Vitreoscilla LB13 can be protected from UV-A by including superoxide dismutase and catalase, separately or in combination, during the exposure, indicatin...

journal_title：FEMS microbiology letters

authors： Biggs LF,Moody AJ

更新日期：2003-07-15 00:00:00

abstract：:A number of hybrids obtained by fusion of protoplasts of complementary auxotrophic strains of Candida albicans were analysed for growth rates, cell volume, DNA content, stability and adherence to human buccal epithelial cells. SDS-PAGE analysis of the cell wall proteins indicated that the hybrid cell walls contained m...

journal_title：FEMS microbiology letters

authors： Law C,Kavanagh K,Whittaker P

更新日期：1994-01-01 00:00:00

abstract：:Tn4560 (8.6 kb) was derived from Tn4556, a Tn3-like element from Streptomyces fradiae. It contains a viomycin resistance gene that has not been used previously for selection in mycobacteria. Tn4560, cloned in a Streptomyces plasmid, was introduced by electroporation into Mycobacterium smegmatis mc(2)155. Tn4560 transp...

journal_title：FEMS microbiology letters

authors： Bhatt A,Stewart GR,Kieser T

更新日期：2002-01-10 00:00:00

abstract：:The plant antimicrobial peptide MiAMP1 from Macadamia integrifolia and the yeast killer toxin peptide WmKT from Williopsis mrakii are structural homologues. Comparative studies of yeast mutants were performed to test their sensitivity to these two antimicrobial peptides. No differences in susceptibility to MiAMP1 were...

journal_title：FEMS microbiology letters

authors： Stephens C,Kazan K,Goulter KC,Maclean DJ,Manners JM

更新日期：2005-02-01 00:00:00

abstract：:The existence in our laboratory of over 10000 Salmonella typhimurium LT2 cultures sealed in agar stab vials for 33-46 years offers an opportunity for evolutionary and mutational studies. In each of 77 vials examined, 10(3)-10(5) colony forming units per vial were recovered (less than 0.01% of the original population) ...

journal_title：FEMS microbiology letters

authors： Edwards K,Linetsky I,Hueser C,Eisenstark A

更新日期：2001-05-30 00:00:00

abstract：:Cytoplasmic precursors of the peptidoglycan biosynthetic pathway were purified from vancomycin-treated, glycopeptide-sensitive and -resistant strains of Enterococcus faecium. Resistance was due to production of a modified precursor, UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-lactate, where lactate was identified on the basi...

journal_title：FEMS microbiology letters

authors： Messer J,Reynolds PE

更新日期：1992-07-01 00:00:00

abstract：:In microbial corrinoid-dependent methyltransferase systems, adventitious Co(I)-corrinoid oxidation halts catalysis and necessitates repair by ATP-dependent reductive activases. RamA, an activase with a C-terminal ferredoxin domain with two [4Fe-4S] clusters from methanogenic archaea, has been far less studied than the...

journal_title：FEMS microbiology letters

authors： Huening KA,Jiang R,Krzycki JA

更新日期：2020-09-01 00:00:00

abstract：:To tackle antimicrobial resistance (AMR) is of outmost importance for the general population to understand the severity and the relevance of different routes of transmission. Respondents of different age groups, educational and occupational backgrounds, area of living, diet and household composition participated in an...

journal_title：FEMS microbiology letters

authors： Alexa Oniciuc EA,Likotrafiti E,Garre A,Ruiz L,Prieto M,Alvarez-Ordóñez A

更新日期：2019-09-01 00:00:00