Abstract:
:Cytoplasmic precursors of the peptidoglycan biosynthetic pathway were purified from vancomycin-treated, glycopeptide-sensitive and -resistant strains of Enterococcus faecium. Resistance was due to production of a modified precursor, UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-lactate, where lactate was identified on the basis of mass of the precursor and on its ability to act as a substrate for D-lactate dehydrogenase after release from the precursor. The presence of the D-lactate residue instead of D-alanine in the terminal position would hinder formation of a vancomycin-precursor complex, without preventing incorporation of the precursor into mature peptidoglycan.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Messer J,Reynolds PEdoi
10.1016/0378-1097(92)90608-qkeywords:
subject
Has Abstractpub_date
1992-07-01 00:00:00pages
195-200issue
1-2eissn
0378-1097issn
1574-6968journal_volume
73pub_type
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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