Modified peptidoglycan precursors produced by glycopeptide-resistant enterococci.

Abstract:

:Cytoplasmic precursors of the peptidoglycan biosynthetic pathway were purified from vancomycin-treated, glycopeptide-sensitive and -resistant strains of Enterococcus faecium. Resistance was due to production of a modified precursor, UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-lactate, where lactate was identified on the basis of mass of the precursor and on its ability to act as a substrate for D-lactate dehydrogenase after release from the precursor. The presence of the D-lactate residue instead of D-alanine in the terminal position would hinder formation of a vancomycin-precursor complex, without preventing incorporation of the precursor into mature peptidoglycan.

journal_name

FEMS Microbiol Lett

authors

Messer J,Reynolds PE

doi

10.1016/0378-1097(92)90608-q

keywords:

subject

Has Abstract

pub_date

1992-07-01 00:00:00

pages

195-200

issue

1-2

eissn

0378-1097

issn

1574-6968

journal_volume

73

pub_type

杂志文章
  • Production in vitro, on different solid culture media, of two distinct exopolysaccharides by a mucoid clinical strain of Burkholderia cepacia.

    abstract::The production of exopolysaccharides (EPSs) by a mucoid clinical isolate of Burkholderia cepacia involved in infections in cystic fibrosis patients, was studied. Depending on the growth conditions, this strain was able to produce two different EPS, namely PS-I and PS-II, either alone or together. PS-I is composed of e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10792.x

    authors: Cérantola S,Montrozier H

    更新日期:2001-08-07 00:00:00

  • An extra large insertion in the polyhydroxyalkanoate synthase from Delftia acidovorans DS-17: its deletion effects and relation to cellular proteolysis.

    abstract::The polyhydroxyalkanoate (PHA) synthase (PhaC(Da)) from Delftia acidovorans DS-17 (formerly Comamonas acidovorans) has a unique large insertion consisting of 40 amino acid residues in the alpha/beta hydrolase fold region. In order to examine whether this insertion is necessary for enzyme function, we generated a mutan...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00930-3

    authors: Tsuge T,Imazu S,Takase K,Taguchi S,Doi Y

    更新日期:2004-02-09 00:00:00

  • Current status of defensins and their role in innate and adaptive immunity.

    abstract::Naturally occurring antimicrobial cationic polypeptides play a major role in innate and adaptive immunity. These polypeptides are found to be either linear and unstructured or structured through disulfide bonds. Among the structured antimicrobial polypeptides, defensins comprise a family of cysteine-rich cationic poly...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.2002.tb10979.x

    authors: Raj PA,Dentino AR

    更新日期:2002-01-02 00:00:00

  • Evaluation of nutritional and physical stress conditions during vegetative growth on conidial production and germination in Ophiocordyceps sinensis.

    abstract::The influence of nutritional and physical stress on sporulation, conidial germination and vegetative biomass of Ophiocordyceps sinensis, one of the most important medicinal fungi in China and now globally, was evaluated using a two-stage culture method. All the treatments, except nutrient deprivation, enhanced conidia...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/1574-6968.12190

    authors: Ren SY,Yao YJ

    更新日期:2013-09-01 00:00:00

  • Gene encoding a replication initiator protein and replication origin of conjugative plasmid pSA1.1 of Streptomyces cyaneus ATCC 14921.

    abstract::pSA1.1 is a 9.1-kb multicopy plasmid originally isolated from Streptomyces cyaneus (formerly S. azureus) ATCC 14921. This plasmid accumulates single-stranded DNA in S. lividans and is therefore considered to replicate by a rolling-circle replication. In the present work, the rep gene encoding the replication initiator...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13305.x

    authors: Yokoyama E,Matsuzaki Y,Doi K,Ogata S

    更新日期:1998-12-01 00:00:00

  • Comparative analysis of gene expression in Streptococcus pneumoniae and Lactococcus lactis.

    abstract::The pFL10 plasmid vector for translational fusions was constructed. pFL10 is based in the promiscuous pLS1 replicon and contains the pC194 cat gene deprived of its transcriptional promoter and Shine-Dalgarno (SD) sequence. Three promoters (Pcit, PpolA and PtetL) from Gram-positive bacteria, inserted in pFL10, were tes...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07182.x

    authors: López de Felipe F,Corrales MA,López P

    更新日期:1994-10-01 00:00:00

  • Quantification of Agrobacterium tumefaciens C58 attachment to Arabidopsis thaliana roots.

    abstract::Agrobacterium tumefaciens is the causal agent of crown gall disease and is a vector for DNA transfer in transgenic plants. The transformation process by A. tumefaciens has been widely studied, but the attachment stage has not been well characterized. Most measurements of attachment have used microscopy and colony coun...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx158

    authors: Petrovicheva A,Joyner J,Muth TR

    更新日期:2017-10-02 00:00:00

  • Molecular characterisation of rough strains of Vibrio cholerae isolated from diarrhoeal cases in India and their comparison to smooth strains.

    abstract::Sixteen of the 18 Vibrio cholerae rough strains isolated from hospitalised diarrhoea patients were found to contain O1 serotype-specific (wbe) genes and all currently known virulence genes. Expression of the regulatory element ToxR was evident in these strains. Cholera toxin production ability of the rough strains was...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(04)00013-8

    authors: De K,Ramamurthy T,Faruque SM,Yamasaki S,Takeda Y,Nair GB,Nandy RK

    更新日期:2004-03-12 00:00:00

  • Subinhibitory concentrations of punicalagin reduces expression of virulence-related exoproteins by Staphylococcus aureus.

    abstract::Staphylococcus aureus produces a number of virulence factors. The major virulence factors exhibited by S aureus include various antigens, enzymes, cytotoxins and exotoxins (e.g. hemolysins, enterotoxins and toxic shock syndrome toxin). In this report, we show the influence of punicalagin on the secretion of exoprotein...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw253

    authors: Mun SH,Kong R,Seo YS,Zhou T,Kang OH,Shin DW,Kwon DY

    更新日期:2016-11-01 00:00:00

  • Evidence for inter- and intraspecies biofilm formation variability among a small group of coagulase-negative staphylococci.

    abstract::Coagulase-negative staphylococci (CoNS) are common bacterial colonizers of the human skin. They are often involved in nosocomial infections due to biofilm formation in indwelling medical devices. While biofilm formation has been extensively studied in Staphylococcus epidermidis, little is known regarding other CoNS sp...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv175

    authors: Oliveira F,Lima CA,Brás S,França Â,Cerca N

    更新日期:2015-10-01 00:00:00

  • A yeast-based genetic screen for identification of pathogenic Salmonella proteins.

    abstract::Salmonella uses type III secretion systems (TTSS) to deliver pathogenic proteins into the host cells. These translocated effectors induce bacterial internalization and intracellular proliferation by targeting important cellular processes that are conserved among eukaryotes. Here, we assessed the feasibility of perform...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01630.x

    authors: Alemán A,Fernández-Piñar P,Pérez-Núñez D,Rotger R,Martín H,Molina M

    更新日期:2009-06-01 00:00:00

  • Electropulsation as an alternative method for protein extraction from yeast.

    abstract::The application of series of high intensity electric pulses to a yeast suspension provoked a considerable release of some cytoplasmic proteins, glutathione reductase, 3-phosphoglycerate kinase and alcohol dehydrogenase. A maximal yield was achieved 3-8 h after pulsation. The electro-induced protein efflux was accelera...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13580.x

    authors: Ganeva V,Galutzov B

    更新日期:1999-05-15 00:00:00

  • Cloning and characterization of the gene encoding penicillin-binding protein A of Streptomyces griseus.

    abstract::An internal segment of the penicillin-binding protein gene, pbpA, of Streptomyces griseus was amplified from genomic DNA using the polymerase chain reaction and used as a hybridization probe to isolate the complete gene from a cosmid library. pbpA encodes a 485 amino acid sequence that conserves three motifs of PBPs, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09403.x

    authors: Jiang H,Kendrick KE

    更新日期:2000-12-01 00:00:00

  • Doc-mediated cell killing in Shigella flexneri using a C1/LacI controlled expression system.

    abstract::In this report we describe the development of a highly stringent and dually regulated promoter system for Shigella flexneri. Dual regulation was provided by utilizing a promoter susceptible to control by the bacteriophage P1 temperature-sensitive C1 repressor that in turn was under the transcriptional control of LacI....

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11396.x

    authors: Schofield DA,Westwater C,Dolan JW,Norris JS,Schmidt MG

    更新日期:2002-10-08 00:00:00

  • Efficacy of species-specific recA PCR tests in the identification of Burkholderia cepacia complex environmental isolates.

    abstract::In this study, we evaluated if recA species-specific PCR assays could be successfully applied to identify environmental isolates of the widespread Burkholderia cepacia complex (Bcc) species. A total of 729 Bcc rhizosphere isolates collected in different samplings were assigned to the species B. cepacia genomovar I (61...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.03.041

    authors: Dalmastri C,Pirone L,Tabacchioni S,Bevivino A,Chiarini L

    更新日期:2005-05-01 00:00:00

  • Amino acid and nucleotide sequence, adjacent genes, and heterologous expression of hiracin JM79, a sec-dependent bacteriocin produced by Enterococcus hirae DCH5, isolated from Mallard ducks (Anas platyrhynchos).

    abstract::The primary structure of a bacteriocin produced by Enterococcus hirae DCH5 was determined by combined amino acid and DNA sequencing. Nucleotide analysis of a 2838-bp DNA fragment of E. hirae DCH5 revealed five putative ORFs. The first orf (hirJM79) encodes a 74-amino-acid peptide containing an N-terminal signal peptid...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00673.x

    authors: Sánchez J,Diep DB,Herranz C,Nes IF,Cintas LM,Hernández PE

    更新日期:2007-05-01 00:00:00

  • ISPsa2, the first mobile genetic element to be described and characterized in the bacterial facultative intracellular pathogen Piscirickettsia salmonis.

    abstract::Piscirickettsia salmonis is a novel, aggressive, facultative Gram-negative bacterium that drastically affects salmon production at different latitudes, with particular impact in southern Chile. Initially, P. salmonis was described as a Rickettsia-like, obligate, intracellular Alphaproteobacteria, but it was reclassifi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02132.x

    authors: Marshall SH,Henríquez V,Gómez FA,Cárdenas C

    更新日期:2011-01-01 00:00:00

  • Esterase from the oil-degrading Acinetobacter lwoffii RAG-1: sequence analysis and over-expression in Escherichia coli.

    abstract::The est gene encoding an esterase from Acinetobacter lwoffii RAG-1 was cloned into E. coli under the control of the PL promoter of the phage lambda. The N-terminal sequence of the first 20 amino acids of the heterologous expressed esterase corresponded to that obtained from the nucleotide sequence. Antibodies prepared...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06462.x

    authors: Alon RN,Gutnick DL

    更新日期:1993-09-15 00:00:00

  • Versatile biosensor vectors for detection and quantification of mercury.

    abstract::Three different whole cell biosensor constructs were made by fusing the mercury inducible promoter, P(mer), and its regulatory gene, merR, from transposon Tn21 with reporter genes luxCDABE, lacZYA, or gfp. In Escherichia coli these biosensor constructs responded to low levels of mercury by producing light, beta-galact...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09413.x

    authors: Hansen LH,Sørensen SJ

    更新日期:2000-12-01 00:00:00

  • SfbII protein, a fibronectin binding surface protein of group A streptococci, is a serum opacity factor with high serotype-specific apolipoproteinase activity.

    abstract::Serum opacity factor (SOF) is produced by group A streptococci belonging to certain M types. SOF cleaves the apolipoprotein component of the high density lipoprotein fraction of serum rendering it insoluble which in turn leads to serum opacity. SfbII protein, a fibronectin binding surface protein cloned from group A s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb08692.x

    authors: Kreikemeyer B,Martin DR,Chhatwal GS

    更新日期:1999-09-15 00:00:00

  • Expression and activity of the Calvin-Benson-Bassham cycle transcriptional regulator CbbR from Acidithiobacillus ferrooxidans in Ralstonia eutropha.

    abstract::Autotrophic fixation of carbon dioxide into cellular carbon occurs via several pathways but quantitatively, the Calvin-Benson-Bassham cycle is the most important. CbbR regulates the expression of the cbb genes involved in CO2 fixation via the Calvin-Benson-Bassham cycle in a number of autotrophic bacteria. A gene pote...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv108

    authors: Esparza M,Jedlicki E,Dopson M,Holmes DS

    更新日期:2015-08-01 00:00:00

  • The expression of the flagellum of Legionella pneumophila is modulated by different environmental factors.

    abstract::Legionella pneumophila, the aetiologic agent of legionnaires' disease, contains a single, monopolar flagellum which is composed of one major subunit, the FlaA protein. Expression studies using a reporter gene fusion of the flaA promoter with the luxAB gene revealed that the flaA expression is not only temperature regu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13603.x

    authors: Heuner K,Brand BC,Hacker J

    更新日期:1999-06-01 00:00:00

  • Separation of Pneumocystis carinii from the lung of the steroid-suppressed rat.

    abstract::Pneumocystis carinii pneumonia was induced in rats by chronic corticosteroid immunosuppression. The parasite was separated from virtually all contaminating host cells using the technique of unit gravity sedimentation. Cellular contamination was routinely below 0.02%. The same technique allowed partial separation of th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90102-v

    authors: Taylor MB,Easmon CS

    更新日期:1990-06-15 00:00:00

  • The adaptation and resistance of Clostridium aminophilum F to the butyrivibriocin-like substance of Butyrivibrio fibrisolvens JL5 and monensin.

    abstract::When the amino acid-fermenting bacterium Clostridium aminophilum F was inoculated into media containing 1 microM monensin or a bacteriocin-like inhibitory substance (BLIS) from Butyrivibrio fibrisolvens JL5, the cultures lagged and growth was not observed for more than 12 h. The monensin- and BLIS-treated cultures eve...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11115.x

    authors: Rychlik JL,Russell JB

    更新日期:2002-03-19 00:00:00

  • The phosphotransferase system gene ptsI in the endophytic bacterium Bacillus cereus is required for biofilm formation, colonization, and biocontrol against wheat sharp eyespot.

    abstract::Natural resistance of wheat plants to wheat sharp eyespot is inadequate, and new strategies for controlling the disease are required. Biological control is an alternative and attractive way of reducing the use of chemicals in agriculture. In this study, we investigated the biocontrol properties of endophytic bacterium...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12438

    authors: Xu YB,Chen M,Zhang Y,Wang M,Wang Y,Huang QB,Wang X,Wang G

    更新日期:2014-05-01 00:00:00

  • Distinct DNA repair pathways involving RecA and nonhomologous end joining in Mycobacterium smegmatis.

    abstract::Mycobacterium smegmatis was used to study the relationship between DNA repair processes involving RecA and nonhomologous end joining (NHEJ). The effect of gene deletions in recA and/or in two genes involved in NHEJ (ku and ligD) was tested on the ability of bacteria to join breaks in plasmids transformed into them and...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00199.x

    authors: Korycka-Machala M,Brzostek A,Rozalska S,Rumijowska-Galewicz A,Dziedzic R,Bowater R,Dziadek J

    更新日期:2006-05-01 00:00:00

  • Dark chocolate as a stable carrier of microencapsulated Akkermansia muciniphila and Lactobacillus casei.

    abstract::The viability of probiotics is affected by several factors during manufacturing, storage and gastrointestinal tract passage. Protecting the probiotics from harmful conditions is particularly critical for oxygen sensitive species like Akkermansia muciniphila, a bacterium which recently has been proposed as a next-gener...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fny290

    authors: Marcial-Coba MS,Saaby L,Knøchel S,Nielsen DS

    更新日期:2019-01-01 00:00:00

  • Expression and purification of Clostridium perfringens beta-toxin glutathione S-transferase fusion protein.

    abstract::The beta-toxin gene from Clostridium perfringens type C was cloned and expressed as a glutathione S-transferase fusion protein in Escherichia coli. The DNA sequence was determined and compared to the type B sequence. Two nucleotide differences were found in the protein coding sequence, resulting in one amino acid diff...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07731.x

    authors: Steinthorsdottir V,Fridriksdottir V,Gunnarsson E,Andrésson OS

    更新日期:1995-08-01 00:00:00

  • Characterization of pRAS1-like plasmids from atypical North American psychrophilic Aeromonas salmonicida.

    abstract::Atypical psychrophilic Aeromonas salmonicida isolates were obtained from farmed and wild fish in Northeastern North America. These bacteria were isolated between 1992 and 2001 and carried tetracycline resistance (Tc(r)) plasmids of approximately 58 kb. The nine isolates had plasmids which could be divided into four gr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.10.039

    authors: Casas C,Anderson EC,Ojo KK,Keith I,Whelan D,Rainnie D,Roberts MC

    更新日期:2005-01-01 00:00:00

  • Experimental evidence of an acetate transporter protein and characterization of acetate activation in aceticlastic methanogenesis of Methanosarcina mazei.

    abstract::Aceticlastic methanogens metabolize acetate to methane and carbon dioxide. The central metabolism and the electron transport chains of these organisms have already been investigated. However, no particular attention has been paid to the mechanism by which acetate enters the archaeal cell. In our study we investigated ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12550

    authors: Welte C,Kröninger L,Deppenmeier U

    更新日期:2014-10-01 00:00:00