Allelic exchange and site-directed mutagenesis probe the contribution of ActA amino-acid variability to phosphorylation and virulence-associated phenotypes among Listeria monocytogenes strains.

abstract：:We have cloned and sequenced the rfaH gene from Salmonella enterica serovar Typhi strain Ty2. The gene showed a high degree of similarity to the rfaH genes from Escherichia coli K-12 and S. enterica serovar Typhimurium. A rfaH mutant was constructed by site-directed mutagenesis. This mutant produced a rough lipopolysa...

journal_title：FEMS microbiology letters

authors： Rojas G,Saldías S,Bittner M,Zaldívar M,Contreras I

更新日期：2001-10-16 00:00:00

abstract：:Using natural rubber latex as the sole source of carbon and energy 50 rubber-degrading bacteria were isolated. Out of those 50 isolates, 33 were identified as Streptomyces species and 8 as Micromonospora species. Screening of 1220 bacteria obtained from different culture collections revealed 46 additional rubber-degra...

journal_title：FEMS microbiology letters

authors： Jendrossek D,Tomasi G,Kroppenstedt RM

更新日期：1997-05-15 00:00:00

abstract：:An identification method by PCR, specific to the Carnobacterium genus, was optimised by testing it on 28 bacterial strains. Primers from the literature were tested to differentiate Carnobacterium strains present among various bacterial species. The DNA of Carnobacterium species (C. alterfunditum, C. divergens, C. fund...

journal_title：FEMS microbiology letters

authors： Cailliez-Grimal C,Miguindou-Mabiala R,Leseine M,Revol-Junelles AM,Millière JB

更新日期：2005-09-01 00:00:00

abstract：:In the transition to the post-petroleum economy, there is a growing demand for novel enzymes with high process performances to replace traditional chemistry with a more 'green' approach. To date, microorganisms encompass the richest source of industrial biocatalysts, but the Earth-living microbiota remains largely unt...

journal_title：FEMS microbiology letters

authors： Berini F,Casciello C,Marcone GL,Marinelli F

更新日期：2017-11-15 00:00:00

abstract：:DNA hybridization using the Synechocystis PCC6803 glucose transporter gene, glcP, revealed a single homologous region in two facultative photoautotrophic strains out of three tested, and none in three obligate autotrophs. In one of the latter, Synechococcus PCC7942, integration of glcP into the chromosome resulted in ...

journal_title：FEMS microbiology letters

authors： Zhang CC,Jeanjean R,Joset F

更新日期：1998-04-15 00:00:00

abstract：:It has been shown that efficient functioning of photosynthesis and respiration in the cyanobacterium Synechocystis PCC 6803 requires the presence of either cytochrome c6 or plastocyanin. In order to check whether the blue copper protein plastocyanin can act as electron donor to cytochrome c oxidase, we investigated th...

journal_title：FEMS microbiology letters

authors： Paumann M,Bernroitner M,Lubura B,Peer M,Jakopitsch C,Furtmüller PG,Peschek GA,Obinger C

更新日期：2004-10-15 00:00:00

abstract：:The filamentous fungi Monascus spp., which have been used in traditional fermented food in Asia for centuries, are well-known producers of a group of bioactive metabolites that are widely used as food additives and nutraceutical supplements worldwide. However, its potential to produce the mycotoxin citrinin poses a th...

journal_title：FEMS microbiology letters

authors： Li L,Shao Y,Li Q,Yang S,Chen F

更新日期：2010-07-01 00:00:00

abstract：:The enzymatic activity of salivary amylase bound to the surface of several species of oral streptococci was determined by the production of acid from starch and by the degradation of maltotetraose to glucose in a coupled, spectrophotometric assay. Most strains able to bind amylase exhibited functional enzyme on their ...

journal_title：FEMS microbiology letters

authors： Douglas CW,Heath J,Gwynn JP

更新日期：1992-04-15 00:00:00

abstract：:Helicobacter pylori genomes contain about 30 hop genes that encode outer membrane proteins. Helicobacter pylori hopQ alleles exhibit a high level of genetic diversity, and two families of hopQ alleles have been described. Type I hopQ alleles are found more commonly in cag-positive H. pylori strains from patients with ...

journal_title：FEMS microbiology letters

authors： Loh JT,Torres VJ,Algood HM,McClain MS,Cover TL

更新日期：2008-12-01 00:00:00

abstract：:The role of the flagellum and chemotactic motility of Vibrio anguillarum for phagocytosis by and intracellular survival in fish macrophages was determined using a wild-type strain, a mutant without the flagellum, a mutant with a truncated flagellum and a non-chemotactic mutant. For all strains, the numbers of intracel...

journal_title：FEMS microbiology letters

authors： Larsen MH,Boesen HT

更新日期：2001-09-25 00:00:00

abstract：:A Ca(2+)/calmodulin-dependent protein kinase (CaMK) gene was cloned and characterized from Arthrobotrys dactyloides, a nematode-trapping fungus. The resulting 373-amino-acid protein, FCaMK, has significant homology to mammalian CaMKs. FCaMK contains a serine/threonine kinase domain followed by a calmodulin-binding dom...

journal_title：FEMS microbiology letters

authors： Tsai PJ,Tu J,Chen TH

更新日期：2002-06-18 00:00:00

abstract：:A 3.5-kb native plasmid (pND103) was identified in Streptococcus thermophilus ST2-1. Preliminary sequence analysis indicated that pND103 belongs to group I S. thermophilus plasmids. A region of approximately 2 kb appears to contain three components: a plus origin of replication (ori) typical of plasmids that replicate...

journal_title：FEMS microbiology letters

authors： Su P,Jury K,Allison GE,Wong WY,Kim WS,Liu CQ,Vancov T,Dunn NW

更新日期：2002-10-29 00:00:00

abstract：:The discovery of nitrite-dependent anaerobic methane oxidation (n-damo) mediated by 'Candidatus Methylomirabilis oxyfera' with nitrite and methane as substrates has connected biogeochemical carbon and nitrogen cycles in a new way. The paddy fields often carry substantial methane and nitrate, thus may be a favorable ha...

journal_title：FEMS microbiology letters

authors： Zhou L,Wang Y,Long XE,Guo J,Zhu G

更新日期：2014-11-01 00:00:00

abstract：:Two interlinked quorum sensing circuits, las and rhl, which control pathogenesis of Pseudomonas aeruginosa are further modulated by numerous regulators, including VqsR (virulence and quorum sensing regulator). High-density oligonucleotide microarrays were used to compare the global expression profile of a wild-type an...

journal_title：FEMS microbiology letters

authors： Juhas M,Wiehlmann L,Salunkhe P,Lauber J,Buer J,Tümmler B

更新日期：2005-01-15 00:00:00

abstract：:In the yearly Internationally Genetically Engineered Machines (iGEM) competition, teams of Bachelor's and Master's students design and build an engineered biological system using DNA technologies. Advising an iGEM team poses unique challenges due to the inherent difficulties of mounting and completing a new biological...

journal_title：FEMS microbiology letters

authors： Tsui J,Meyer AS

更新日期：2016-07-01 00:00:00

abstract：:We report the effects of dissolved oxygen (DO) concentration and iron addition on gene expression of Magnetospirillum gryphiswaldense MSR-1 cells during fermentations, focusing on 0.25-24 h after iron addition. The DO was strictly controlled at 0.5% or 5% O2, and compared with aerobic condition. Uptake of iron (and fo...

journal_title：FEMS microbiology letters

authors： Zhuang S,Anyaogu DC,Kasama T,Workman M,Mortensen UH,Hobley TJ

更新日期：2017-06-15 00:00:00

abstract：:Glutamate uptake in the Gram-positive Corynebacterium glutamicum is mediated via a binding protein-dependent transport system, which is encoded by the gluABCD gene cluster. Cloning of these genes in an expression vector and subsequent transformation of the resulting plasmid allows different strains of the Gram-negativ...

journal_title：FEMS microbiology letters

authors： Burkovski A,Krämer R

更新日期：1995-04-01 00:00:00

abstract：:A bacterium strain, which is capable of degrading monocrotophos, was isolated from sludge collected from the bottom of a wastewater treatment system of a chemical factory, and named M-1. On the basis of the results of the cellular morphology, physiological and chemotaxonomic characteristics and phylogenetic similarity...

journal_title：FEMS microbiology letters

authors： Jia KZ,Cui ZL,He J,Guo P,Li SP

更新日期：2006-10-01 00:00:00

abstract：:The genes encoding the desulforubidin type of dissimilatory sulfite reductase (Dsr) from the sulfate-reducing bacteria Desulfobacter vibrioformis and Desulfobulbus rhabdoformis were cloned and sequenced. Similar to the genes for dissimilatory sulfite reductase from the genera Archaeoglobus, Desulfovibrio and Desulfoto...

journal_title：FEMS microbiology letters

authors： Larsen,Lien T,Birkeland NK

更新日期：2000-05-01 00:00:00

abstract：:The effects of specific inhibitors of glycoprotein trimming reactions on Junin virus (JV) replication were investigated. Bromoconduritol, an inhibitor of glucosidase II, significantly reduced infective virus production (DE50: 1.1 mM) and viral protein expression. Neither 1-deoxynojirimycin, an inhibitor of both glucos...

journal_title：FEMS microbiology letters

authors： Silber AM,Candurra NA,Damonte EB

更新日期：1993-05-01 00:00:00

abstract：:The purpose of this study was to generate highly specific serological reagents for the quantitative identification of Actinomyces naeslundii in clinical samples, in particular dental plaque. Balb/c mice were immunized with pasteurized human A. naeslundii strains representing different genospecies and serotypes. Ten hy...

journal_title：FEMS microbiology letters

authors： Thurnheer T,Guggenheim B,Gmür R

更新日期：1997-05-15 00:00:00

abstract：:Protein A from the Staphylococcus aureus strain V8 has a molecular mass about 8000 Da less than that of known proteins A. The corresponding gene was cloned and expressed in Escherichia coli. Sequence analysis of this structurally new protein A revealed that it lacked an IgG-binding domain (58 amino acids), and that it...

journal_title：FEMS microbiology letters

authors： Finck-Barbançon V,Prevost G,Mazurier I,Piemont Y

更新日期：1992-02-01 00:00:00

abstract：:Porphyromonas gingivalis produces a trypsin-like enzyme, Protease I, which is thought to be an important virulence determinant of the organism in adult periodontal disease. Protease I is transiently inhibited by physiological inhibitors of human thrombin. The aim of the present work was to establish whether Protease I...

journal_title：FEMS microbiology letters

authors： Curtis MA,Macey M,Slaney JM,Howells GL

更新日期：1993-06-15 00:00:00

abstract：:The alternative sigma factor sigmaH has been shown to play an important role in stress response and morphological differentiation in Streptomyces coelicolor. Its gene, sigH, is located in an operon with the gene encoding proposed anti-sigma factor UshX, and one of the promoters directing expression of the operon is de...

journal_title：FEMS microbiology letters

authors： Sevcikova B,Kormanec J

更新日期：2002-04-09 00:00:00

abstract：:The lipid modification and processing of truncated mutants of the colicin A lysis protein were observed after overproduction by Escherichia coli bacteria, but at a rate far slower than that of the wild-type. The unmodified precursor form of the mutants was stable over hour(s). The truncated mutants provoked lethality,...

journal_title：FEMS microbiology letters

authors： Cavard D

更新日期：1994-04-01 00:00:00

abstract：:We developed an improved HEp-2 cell assay method for the detection of Bacillus cereus toxin, which affects the proliferation of HEp-2 cells. The cytostatic toxin was stable upon exposure to heat, pH 2, pH 11 and trypsin, which suggests it is an emetic. Using the HEp-2 cell assay, we examined the distribution and conta...

journal_title：FEMS microbiology letters

authors： Mikami T,Horikawa T,Murakami T,Matsumoto T,Yamakawa A,Murayama S,Katagiri S,Shinagawa K,Suzuki M

更新日期：1994-06-01 00:00:00

abstract：:A fast screening method was developed to assess the pathogenicity of a diverse collection of environmental and clinical Burkholderia cepacia complex isolates in the nematode Caenorhabditis elegans. The method was validated by comparison with the standard slow-killing assay. We observed that the pathogenicity of B. cep...

journal_title：FEMS microbiology letters

authors： Cardona ST,Wopperer J,Eberl L,Valvano MA

更新日期：2005-09-01 00:00:00

abstract：:A serum-susceptible, guinea-pig chamber-passaged, laboratory strain (BS4 (agar)) of Neisseria gonorrhoeae was converted to serum resistance by incubation with cytidine 5-monophospho-N-acetyl neuraminic acid (CMP-NANA) and examined by electron microscopy after staining with ruthenium-red. In contrast to serum susceptib...

journal_title：FEMS microbiology letters

authors： Fox AJ,Curry A,Rowland PL,Lancaster S,Jones DM,Parsons NJ,Cole JA,Smith H

更新日期：1990-01-01 00:00:00

abstract：:Many antibiotic-producing actinomycetes possess at least one ABC (ATP-binding cassette) transporter which forms part of the antibiotic biosynthetic pathway and in most cases confers resistance to the drug in an heterologous host. Three types of antibiotic ABC transporters have been so far described in producer organis...

journal_title：FEMS microbiology letters

authors： Méndez C,Salas JA

更新日期：1998-01-01 00:00:00

abstract：:The ability of Candida albicans to adhere to subendothelial extracellular matrix (ECM) may be important in the pathogenesis of disseminated candidiasis. ECM proteins, such as fibronectin, laminin, and types I and IV collagen bind C. albicans avidly. These proteins all possess heparin-binding domains. The influence of ...

journal_title：FEMS microbiology letters

authors： Klotz SA,Smith RL

更新日期：1992-12-01 00:00:00