Functional expression of the glutamate uptake system from Corynebacterium glutamicum in Escherichia coli.

abstract：:Brucella melitensis possesses an operon with two components: the response regulator OtpR and a putative cAMP-dependent protein kinase regulatory subunit encoded by the BMEI0067 gene. In the previous study, the function of OtpR has been studied, while little is known about the function of the BMEI0067 gene. Using a bio...

journal_title：FEMS microbiology letters

authors： Liu WJ,Dong H,Peng XW,Wu QM

更新日期：2014-07-01 00:00:00

abstract：:Brucella usually carry two highly homologous genes (omp2a and omp2b) for porin-like proteins. In several B. abortus biovars the omp2a gene has a large deletion compared to other Brucella omp2's. In this study we have measured Omp2 pore activity in planar bilayers. Omp2b exhibits well-defined trimeric channel activity ...

journal_title：FEMS microbiology letters

authors： Mobasheri H,Ficht TA,Marquis H,Lea EJ,Lakey JH

更新日期：1997-10-01 00:00:00

abstract：:A novel alphaproteobacterium, designated KMU-156T, was isolated from seawater collected on the coast of Jeju Island, Republic of Korea, and its phylogenetic position was determined using a polyphasic taxonomic approach. Strain KMU-156T was Gram-stain-negative, strictly aerobic, apricot-colored, rod-shaped, non-motile ...

journal_title：FEMS microbiology letters

authors： Yoon J

更新日期：2019-08-01 00:00:00

abstract：:In spite of being dispensable, O-antigens are believed to facilitate various cellular processes and alter antibiotic sensitivities. Escherichia coli K-12 (CS109) strains are lacking in O-antigens and are reported to be sensitive to antibiotics. To our surprise, E. coli 2443 (expressing O8-antigen) manifested two- to f...

journal_title：FEMS microbiology letters

authors： Sarkar SK,Ghosh AS

更新日期：2008-05-01 00:00:00

abstract：:The riboflavin kinase encoding gene ribR is situated within a 12 genes locus ytmI-ytnM of the Bacillus subtilis chromosome. Here we demonstrate that ribR is transcribed as part of a 10 kb ytmI-ytnM operon. The riboflavin overproduction phenotype of B. subtilis ribC mutant strains, which is a result of the strongly red...

journal_title：FEMS microbiology letters

authors： Solovieva IM,Kreneva RA,Errais Lopes L,Perumov DA

更新日期：2005-02-01 00:00:00

abstract：:In Salmonella typhimurium the metE and metR promoters overlap and are divergently transcribed. Three tandem repeats of an 8 bp sequence defined previously as the metE operator site for MetJ-mediated repression also overlap the -35 region of the metR promoter. Starting with a metE-lacZ.metR-galK double gene fusion, sit...

journal_title：FEMS microbiology letters

authors： Wu WF,Urbanowski ML,Stauffer GV

更新日期：1993-04-01 00:00:00

abstract：:The streptokinase gene of Streptococcus equisimilis H46 was inactivated by plasmid insertion mutagenesis to study the relationship between elaboration of streptokinase and acquisition of cell-associated plasmin activity after incubation of wild-type and mutant cells in media containing plasminogen or plasmin. The resu...

journal_title：FEMS microbiology letters

authors： Malke H,Mechold U,Gase K,Gerlach D

更新日期：1994-02-01 00:00:00

abstract：:Legionella pneumophila is known as a facultative intracellular parasite of free-living soil and freshwater amoebae, of which several species have been shown to support the growth of the pathogenic bacteria. We report for the first time the behaviour of two strains (c2c and Z503) of the amoeba Willaertia magna towards ...

journal_title：FEMS microbiology letters

authors： Dey R,Bodennec J,Mameri MO,Pernin P

更新日期：2009-01-01 00:00:00

abstract：:Occurrence of tetracycline resistance genes encoding ribosomal protection proteins was examined in 151 tetracycline-resistant bacterial isolates from fish and seawater at coastal aquaculture sites in Japan and Korea. The tet(M) gene was detected in 34 Japanese and Korean isolates, which included Vibrio sp., Lactococcu...

journal_title：FEMS microbiology letters

authors： Kim SR,Nonaka L,Suzuki S

更新日期：2004-08-01 00:00:00

abstract：:Cell differentiation is one of the marks of multicellular organisms. Terminally specialised nitrogen-fixing cells, termed heterocysts, evolved in filamentous cyanobacteria more than 2 Gya. The development of their spacing pattern has been thoroughly investigated in model organisms such as Anabaena sp. PCC 7120. This p...

journal_title：FEMS microbiology letters

authors： Antonaru LA,Nürnberg DJ

更新日期：2017-08-15 00:00:00

abstract：:Shigella strains are human pathogens and their identification is usually based on their O-antigens. The O-antigen gene cluster of Shigella boydii O11 was sequenced. All the expected genes for the synthesis of the O-antigen were identified on the basis of homology and genes for the biosynthesis of dTDP-l-Rhamnose, gene...

journal_title：FEMS microbiology letters

authors： Tao J,Feng L,Guo H,Li Y,Wang L

更新日期：2004-05-01 00:00:00

abstract：:In haloarchaea, a cluster of three genes is localized directly adjacent to the major replication origin, and, hence, the encoded proteins were annotated as 'origin-associated proteins' (Oap). However, prior to this study, no experimental data were available for these conserved hypothetical proteins. Bioinformatic anal...

journal_title：FEMS microbiology letters

authors： Wolters M,Borst A,Pfeiffer F,Soppa J

更新日期：2019-11-01 00:00:00

abstract：:While there is an extensive database of genes encoding ammonia monooxygenase (amo) found in the ammonia-oxidizing beta-proteobacteria, few amo sequences are available representing the gamma-proteobacteria. We sequenced the complete amo operon (amoCAB) for Nitrosococcus oceani (ATCC 19707), a marine, autotrophic, ammon...

journal_title：FEMS microbiology letters

authors： Alzerreca JJ,Norton JM,Klotz MG

更新日期：1999-11-01 00:00:00

abstract：:We have constructed a very efficient synthesis and secretion system for cholera toxin B subunit (CTB) of Vibrio cholerae 569B using Bacillus-brevis. The constructed expression-secretion vector has the multiple promoters and the signal peptide coding region of the mwp gene, a structural gene for one of the major cell w...

journal_title：FEMS microbiology letters

authors： Ichikawa Y,Yamagata H,Tochikubo K,Udaka S

更新日期：1993-08-01 00:00:00

abstract：:Two fungal isolates, formerly classified as Verticillium alboatrum and proposed as forming the basis of a new sub-group ('Group 2') within the species, have been shown to be non-pathogenic to known hosts of V. alboatrum and, on the basis of molecular evidence, to be closely related to Verticillium psalliotae and Verti...

journal_title：FEMS microbiology letters

authors： Carder JH,Barbara DJ

更新日期：1999-01-01 00:00:00

abstract：:A composite transposon is a mobile genetic element consisting of two insertion sequences (ISs) flanking a segment of cargo DNA often containing antibiotic resistance (AR) genes. Composite transposons can move as a discreet unit. There have been recently several reports on a novel mechanism of movement of an IS26-based...

journal_title：FEMS microbiology letters

authors： Tansirichaiya S,Mullany P,Roberts AP

更新日期：2016-09-01 00:00:00

abstract：:We recently described adhesion to and invasion of bovine mammary gland cells by Staphylococcus aureus in vitro. Here, we show that the levels of adhesion and invasion are dependent on the bacterial growth phase and are controlled by the agr locus. Incubation of exponential growth phase cells of S. aureus with mammary ...

journal_title：FEMS microbiology letters

authors： Lammers A,Nuijten PJ,Smith HE

更新日期：1999-11-01 00:00:00

abstract：:We isolated nickel-resistant bacterium from soil in order to identify a novel nickel resistance determinant. Using 16S rRNA gene sequencing, an isolate was identified as Enterobacter sp. Ni15. This species showed a medium-level (resistant to up to 10 mM) nickel resistance in nutrient-rich media. Enterobacter sp. Ni15 ...

journal_title：FEMS microbiology letters

authors： Lee YK,Chang HH,Lee HJ,Park H,Lee KH,Joe MH

更新日期：2006-04-01 00:00:00

abstract：:A cordycepin-resistant mutant strain of Saccharomyces cerevisiae (CD-R2) was found to be deficient in adenosine kinase. This mutant accumulated S-adenosylhomocysteine during growth in the presence of exogenous adenosine and it grew in a pseudohyphal manner in the presence of this nucleotide. ...

journal_title：FEMS microbiology letters

authors： Iwashima A,Ogata M,Nosaka K,Nishimura H,Hasegawa T

更新日期：1995-03-15 00:00:00

abstract：:Serine hydroxymethyltransferase (SHMT) is a key enzyme in cellular one-carbon pathway and has been studied in many living organisms from bacteria to higher plants and mammals. However, biochemical and molecular characterization of SHMT from photoautotrophic microorganisms remains a challenge. Here, we isolated the SHM...

journal_title：FEMS microbiology letters

authors： Waditee-Sirisattha R,Sittipol D,Tanaka Y,Takabe T

更新日期：2012-08-01 00:00:00

abstract：:This study reports on the antifungal activities of statins combined with an antifungal compound secreted by Penicillium chrysogenum, PAF. Several species belonging in the class Zygomycetes are considered to be agents of human or animal mycoses; other species have significance as postharvest plant pathogens. In the pre...

journal_title：FEMS microbiology letters

authors： Galgóczy L,Papp T,Lukács G,Leiter E,Pócsi I,Vágvölgyi C

更新日期：2007-05-01 00:00:00

abstract：:An open reading frame located in the tyrB-uvrA intergenic region of the Escherichia coli MG1655 chromosome was identified as encoding the class B acid phosphatase of this species on the basis of cloning and expression experiments. A protocol for purification of the enzyme (named AphA) was developed, and its properties...

journal_title：FEMS microbiology letters

authors： Thaller MC,Schippa S,Bonci A,Cresti S,Rossolini GM

更新日期：1997-01-15 00:00:00

abstract：:Phycoerythrin-containing Synechococcus strains are unicellular cyanobacteria that are of great ecological importance in the marine environment. These organisms are known to be susceptible to infection by cyanophages (viruses that infect cyanobacteria). The infection cycle takes several hours and during this time the c...

journal_title：FEMS microbiology letters

authors： Shan J,Jia Y,Clokie MR,Mann NH

更新日期：2008-06-01 00:00:00

abstract：:We have cloned and sequenced the rfaH gene from Salmonella enterica serovar Typhi strain Ty2. The gene showed a high degree of similarity to the rfaH genes from Escherichia coli K-12 and S. enterica serovar Typhimurium. A rfaH mutant was constructed by site-directed mutagenesis. This mutant produced a rough lipopolysa...

journal_title：FEMS microbiology letters

authors： Rojas G,Saldías S,Bittner M,Zaldívar M,Contreras I

更新日期：2001-10-16 00:00:00

abstract：:Growth of strains of Salmonella enteritidis and Salmonella pullorum on Hektoen agar has been reported to influence the expression of long-chain lipopolysaccharide and motility respectively. In this study we used a panel of strains of S. enteritidis and S. pullorum to investigate these phenomena. Culture on Hektoen aga...

journal_title：FEMS microbiology letters

authors： Chart H,Rowe B

更新日期：1998-06-15 00:00:00

abstract：:Members of the genus Actinoplanes are considered to be representative of motile actinomycetes. To infer the flagellar diversity of Actinoplanes species, novel degenerate primers were designed for the flagellin (fliC) gene. The fliC gene of 21 Actinoplanes strains was successfully amplified and classified into two grou...

journal_title：FEMS microbiology letters

authors： Yamamura H,Hanawa K,Kusunoki M,Nakamura K,Nakagawa Y,Ishida Y,Hamada M,Otoguro M,Tamura T,Fujita N,Hayakawa M

更新日期：2011-11-01 00:00:00

abstract：:The fatty acid composition during the transformation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigotes (metacyclogenesis) was analysed by gas-liquid chromatography and mass spectrometry. Significant qualitative and quantitative changes in the fatty acid composition occurred during incubation of epimas...

journal_title：FEMS microbiology letters

authors： Esteves MG,Gonzales-Perdomo M,Alviano CS,Angluster J,Goldenberg S

更新日期：1989-05-01 00:00:00

abstract：:During programs for the selection of enological yeasts, several hundred natural isolates are usually screened. The scope of these operations is to isolate strains possessing good fermentative properties without necessarily arriving at a precise species designation: in other words, to detect strains belonging to the Sa...

journal_title：FEMS microbiology letters

authors： Nardi T,Carlot M,De Bortoli E,Corich V,Giacomini A

更新日期：2006-11-01 00:00:00

abstract：:Symbioses between phototrophs and heterotrophs (a.k.a 'photosymbioses') are extremely common, and range from loose and temporary associations to obligate and highly specialized forms. In the history of life, the most transformative was the 'primary endosymbiosis,' wherein a cyanobacterium was engulfed by a eukaryote a...

journal_title：FEMS microbiology letters

authors： Gavelis GS,Gile GH

更新日期：2018-10-01 00:00:00

abstract：:We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to...

journal_title：FEMS microbiology letters

authors： Devillard E,Newbold CJ,Scott KP,Forano E,Wallace RJ,Jouany JP,Flint HJ

更新日期：1999-12-01 00:00:00