A rapid method for differentiating Saccharomyces sensu stricto strains from other yeast species in an enological environment.

Abstract:

:During programs for the selection of enological yeasts, several hundred natural isolates are usually screened. The scope of these operations is to isolate strains possessing good fermentative properties without necessarily arriving at a precise species designation: in other words, to detect strains belonging to the Saccharomyces sensu stricto complex. In the present study, a pair of primers, designed within the variable D1/D2 region of the 26S subunit of ribosomal yeast RNA, have been constructed. These generate an amplification fragment of 471 bp that is specific for the seven Saccharomyces sensu stricto species, while no signal was obtained for Saccharomyces sensu lato strains (17 species) or for another 18 selected species commonly found in enological environments. A second pair of primers was also constructed, within the 18S rRNA gene, composed of perfectly conserved sequences common for all 42 yeast species examined, which generate a 900 bp (c.) band for all strains. This was used as a positive experimental control in multiplex PCR analysis using all four primers.

journal_name

FEMS Microbiol Lett

authors

Nardi T,Carlot M,De Bortoli E,Corich V,Giacomini A

doi

10.1111/j.1574-6968.2006.00450.x

subject

Has Abstract

pub_date

2006-11-01 00:00:00

pages

168-73

issue

2

eissn

0378-1097

issn

1574-6968

pii

FML450

journal_volume

264

pub_type

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