Characterization of the desulforubidin operons from Desulfobacter vibrioformis and Desulfobulbus rhabdoformis.

Abstract:

:The genes encoding the desulforubidin type of dissimilatory sulfite reductase (Dsr) from the sulfate-reducing bacteria Desulfobacter vibrioformis and Desulfobulbus rhabdoformis were cloned and sequenced. Similar to the genes for dissimilatory sulfite reductase from the genera Archaeoglobus, Desulfovibrio and Desulfotomaculum the dsr genes were found to form an operon, dsrABD, where dsrA and dsrB encode the structural subunits, alpha and beta, of Dsr, respectively. dsrD encodes a conserved unknown protein apparently restricted to sulfate-reducing species. In Desulfobacter vibrioformis a fourth gene, designated dsrN, was found downstream of dsrD forming a contiguous operon, dsrABDN. DsrN showed significant sequence homology to cobyrinic a,c-diamide synthase, which is involved in the biosynthesis of vitamin B12. A function for DsrN in amidation of siroheme is likely. Analysis of the dsrAB-encoded proteins confirmed that the high conservation observed for other types of dissimilatory sulfite reductase is also found in desulforubidin. The use of Dsr sequences in unravelling the phylogeny of sulfate-reducing bacteria is discussed.

journal_name

FEMS Microbiol Lett

authors

Larsen,Lien T,Birkeland NK

doi

10.1111/j.1574-6968.2000.tb09079.x

keywords:

subject

Has Abstract

pub_date

2000-05-01 00:00:00

pages

41-6

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378109700001130

journal_volume

186

pub_type

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