Abstract:
:A plasmid-based food-grade vector system was developed for Lactococcus lactis by exploiting the genes for lactose metabolism. L. lactis MG5267 is a plasmid-free strain containing the entire lactose operon as a chromosomal insertion. The lacF gene was deleted from this strain by a double cross-over homologous recombination event. The lacF-deficient strain produced a Lac- phenotype on indicator agar. A cloned copy of the lacF gene expressed on a plasmid was capable of complementing the lacF-deficient strain resulting in a Lac+ phenotype. This stably maintained system fits the requirements of a self-selecting vector system and has the potential to be exploited in the food industry.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
MacCormick CA,Griffin HG,Gasson MJdoi
10.1111/j.1574-6968.1995.tb07457.xsubject
Has Abstractpub_date
1995-03-15 00:00:00pages
105-9issue
1-2eissn
0378-1097issn
1574-6968journal_volume
127pub_type
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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