Enzymic activity of salivary amylase when bound to the surface of oral streptococci.

abstract：:We have established an improved large deletion method in Escherichia coli genome using a combination of two different recombination systems, lambda Red and Cre/loxP. The loxP site could be rapidly and efficiently integrated in the genome by lambda Red and large deletions of both 117- and 165-kbp regions could be gener...

journal_title：FEMS microbiology letters

authors： Fukiya S,Mizoguchi H,Mori H

更新日期：2004-05-15 00:00:00

abstract：:Six strains isolated from fermented food were identified as Weissella species by 16S rDNA sequencing, clustering with the species pair W. confusa/W. cibaria. The strains were analysed for growth on glucose, xylose and xylooligosaccharides (XOS). All strains were xylose positive using the API CHL 50 test. Growth on XOS...

journal_title：FEMS microbiology letters

authors： Patel A,Falck P,Shah N,Immerzeel P,Adlercreutz P,Stålbrand H,Prajapati JB,Holst O,Nordberg Karlsson E

更新日期：2013-09-01 00:00:00

abstract：:The enteric microogranisms Salmonella, Escherichia coli and Shigella flexneri prefer to grow in neutral pH environments. They nevertheless experience dramatic pH fluctuations in nature and during pathogenesis. In response to environmental encounters with acid, these organisms have evolved complex, inducible acid survi...

journal_title：FEMS microbiology letters

authors： Bearson S,Bearson B,Foster JW

更新日期：1997-02-15 00:00:00

abstract：:mlrA is the only microcystin-degrading gene detected in Sphingomonas sp. MJ-PV. The gene has an extremely rare nucleotide sequence and homologous genes have not yet been discovered in the DNA database. We discovered the existence of a gene homologous to mlrA in new microcystin-degrading bacteria, MD-1 and Y2. These st...

journal_title：FEMS microbiology letters

authors： Saito T,Okano K,Park HD,Itayama T,Inamori Y,Neilan BA,Burns BP,Sugiura N

更新日期：2003-12-12 00:00:00

abstract：:A series of isogenic mutants lacking either the O1 (O-:K66) or K66 (O1:K-) antigens or both (O-:K-), some of which had additional defects in their LPS core polysaccharide was used to examine the interaction between polymorphonuclear leucocytes (PMNLs) and K. pneumoniae serotype O1:K66. In the absence of serum compleme...

journal_title：FEMS microbiology letters

authors： Williams P,Ciurana B,Camprubi S,Tomas JM

更新日期：1990-06-01 00:00:00

abstract：:Pseudomonas syringae pv. tomato DC3000, a plant pathogenic gram-negative bacterium, employs the type III secretion system (T3SS) to cause disease in tomato and Arabidopsis and to induce the hypersensitive response in nonhost plants. The expression of T3SS is regulated by the HrpL extracytoplasmic sigma factor. Express...

journal_title：FEMS microbiology letters

authors： Jovanovic M,Lawton E,Schumacher J,Buck M

更新日期：2014-07-01 00:00:00

abstract：:The polymerase chain reaction was used to search for hepatitis B virus (HBV)-DNA sequences in the sera of HBsAg-negative Senegalese patients suffering from liver cirrhosis or liver cancer. Amplified HBV-DNA sequences were detected by hybridization with a digoxigenin-labelled HBV-DNA probe. HBV-DNA was detected in 17% ...

journal_title：FEMS microbiology letters

authors： Coursaget P,Le Cann P,Leboulleux D,Diop MT,Bao O,Coll AM

更新日期：1991-09-15 00:00:00

abstract：:The gacA gene of the biocontrol strain Pseudomonas fluorescens CHA0 codes for a response regulator which, together with the sensor kinase GacS (=LemA), is required for the production of exoenzymes and secondary metabolites involved in biocontrol, including hydrogen cyanide (HCN). A gacA multicopy suppressor was isolat...

journal_title：FEMS microbiology letters

authors： Blumer C,Haas D

更新日期：2000-06-01 00:00:00

abstract：:It is shown here by flow cytometry that Bacillus subtilis YabA negatively regulates the timing of replication initiation. When the level of YabA was reduced, replication began at a decreased cell mass and when the level was increased, initiation was delayed. Synchrony of replication initiation was also disrupted at lo...

journal_title：FEMS microbiology letters

authors： Hayashi M,Ogura Y,Harry EJ,Ogasawara N,Moriya S

更新日期：2005-06-01 00:00:00

abstract：:The molecular genetics of indole-3-acetic (IAA) synthesis and regulation in Azospirillum brasilense was investigated in this study. Tn5 mutagenesis was performed and five mutants with decreased IAA production were isolated. Five Tn5-inserted genes from these mutants were cloned and sequenced. Four genes were reported ...

journal_title：FEMS microbiology letters

authors： Xie B,Xu K,Zhao HX,Chen SF

更新日期：2005-07-01 00:00:00

abstract：:When challenged by stresses such as starvation, the soil bacterium Bacillus subtilis produces an endospore surrounded by a proteinaceous coat composed of >70 proteins that are organized into three main layers: an amorphous undercoat, lightly staining lamellar inner coat and electron-dense outer coat. This coat protect...

journal_title：FEMS microbiology letters

authors： Müllerová D,Krajčíková D,Barák I

更新日期：2009-10-01 00:00:00

abstract：:We have constructed a fungal subtractive suppressive library enriched in genes expressed during appressorium maturation in Magnaporthe grisea. Sequencing of 250 clones from the subtracted appressorium cDNA library revealed 142 unique genes, represented by 155 non-redundant ESTs (expressed sequence tags). Of these ESTs...

journal_title：FEMS microbiology letters

authors： Lu JP,Liu TB,Lin FC

更新日期：2005-04-01 00:00:00

abstract：:A critical step in the process of metagenome analysis is to screen for clones that contain specific genes among a large number of clones. To form one of the sequence-based screening tools of a metagenome library, we designed a format of microarray [metagenome microarray (MGA)] that is arrayed with fosmid library clone...

journal_title：FEMS microbiology letters

authors： Park SJ,Kang CH,Chae JC,Rhee SK

更新日期：2008-07-01 00:00:00

abstract：:Bacterial aerotaxis is a rapid response towards or away from oxygen. Here we report on the use of computer-assisted motion analysis coupled to flash photolysis of caged oxygen to quantify aerotactic responses in bacteria. The caged compound (mu-peroxo)(mu-hydroxo)bis[bis(bipyridyl) cobalt(III)] perchlorate liberates m...

journal_title：FEMS microbiology letters

authors： Yu HS,Saw JH,Hou S,Larsen RW,Watts KJ,Johnson MS,Zimmer MA,Ordal GW,Taylor BL,Alam M

更新日期：2002-12-17 00:00:00

abstract：:We investigated the hemolytic activity of Escherichia coli strain EC901 carrying plasmid pBJN406 containing genes draA-E involved in expression of the mannose-resistant Dr hemagglutinin, and in its isogenic insertion mutants devised with Tn5, Tn3, and TnphoA. While E. coli BN406 displayed rapid hemolytic activity agai...

journal_title：FEMS microbiology letters

authors： Goluszko P,Nowicki S,Kaul AK,Pham T,Nowicki BJ

更新日期：1995-07-15 00:00:00

abstract：:A CO-reactive hemoprotein was purified from the mitochondrial membrane fraction of Tetrahymena pyriformis. It showed absorption peaks at 615 and 455 nm in the reduced form and an alpha peak at 565 nm in the pyridine ferrohemochrome spectrum. Although the spectral properties were apparently similar to those of 'cytochr...

journal_title：FEMS microbiology letters

authors： Inokuchi A,Fukumori Y

更新日期：1993-08-15 00:00:00

abstract：:A unique genomic DNA fragment was isolated from Nitrosomonas europaea ATCC 19718. Based on the sequence of this fragment, oligonucleotide primers for polymerase chain reaction amplification were prepared which amplify sequences of 775 and 658 bp. The predicted DNA fragments were both amplified from the genome of N. eu...

journal_title：FEMS microbiology letters

authors： Nejidat A,Abeliovich A

更新日期：1994-07-01 00:00:00

abstract：:The aim of this study was to understand the pathogenesis of motile aeromonas septicemia caused by an emergent, high virulent Aeromonas hydrophila (vAh) in channel catfish, Ictalurus punctatus Adipose fin clipped catfish were challenged with vAh using a waterborne challenge method, and the distribution of vAh over a ti...

journal_title：FEMS microbiology letters

authors： Zhang D,Moreira GS,Shoemaker C,Newton JC,Xu DH

更新日期：2016-05-01 00:00:00

abstract：:We recently identified the phaG(Pp) gene encoding (R)-3-hydroxydecanoyl-ACP:CoA transacylase in Pseudomonas putida, which directly links the fatty acid de novo biosynthesis and polyhydroxyalkanoate (PHA) biosynthesis. An open reading frame (ORF) of which the deduced amino acid sequence shared about 57% identity with P...

journal_title：FEMS microbiology letters

authors： Hoffmann N,Steinbüchel A,Rehm BH

更新日期：2000-03-15 00:00:00

abstract：:We present evidence that the anthrax toxin lethal factor binds multiple zinc atoms. Results from atomic adsorption spectroscopy indicate that lethal factor contains approximately three zinc atoms per toxin molecule. Lethal factor treated with EDTA and o-phenanthroline contained a similar number of zinc atoms, indicati...

journal_title：FEMS microbiology letters

authors： Kochi SK,Schiavo G,Mock M,Montecucco C

更新日期：1994-12-15 00:00:00

abstract：:Streptomyces netropsis SD-07, the producer of novel polyene macrolide antifungal antibiotics, was isolated from soil. For the investigation of the functions of its biosynthesis genes and regulation mechanisms, a genetic operating system is necessary. In this study, we successfully transferred the plasmid DNA of pSET15...

journal_title：FEMS microbiology letters

authors： Wang XK,Jin JL

更新日期：2014-08-01 00:00:00

abstract：:In Corynebacterium glutamicum the promoter of the araBAD Escherichia coli gene is positively regulated by both arabinose and the araC gene product, as it is the case in the natural host. If the L-arabinose inducer and an active araC gene are present, significant amounts of araBAD promoter expression take place in the ...

journal_title：FEMS microbiology letters

authors： Ben-Samoun K,Leblon G,Reyes O

更新日期：1999-05-01 00:00:00

abstract：:The PenJ protein of the penicillinase gene (penP) expression system from Bacillus licheniformis is an antirepressor and membrane receptor for beta-lactam antibiotics. A putative beta-lactam antibiotic binding site including Ser402 and Lys405, which are homologous to the conserved sequence for the beta-lactam binding s...

journal_title：FEMS microbiology letters

authors： Takagi M,Ohta T,Johki S,Imanaka T

更新日期：1993-06-01 00:00:00

abstract：:A bacterial strain, designated as TSB-6, was isolated from the sediments of a Tantloi (India) hot spring at 65 °C. The strain showed 98% 16S rRNA gene sequence similarity with Anoxybacillus kualawohkensis strain KW12 and was found to grow optimally at 37 °C. However, growing cells, cell suspensions, and cell-free extr...

journal_title：FEMS microbiology letters

authors： Paul SC,Jain P,Mitra J,Dutta S,Bhattacharya P,Bal B,Bhattacharyya D,Das Gupta S,Pal S

更新日期：2012-06-01 00:00:00

abstract：:We report the draft genome sequence from Aeromonas salmonicida sp. strain CBA100, which was characterized as an antibiotic-resistant bacterium isolated from infected rainbow trout. The total size of the genome is 4,788,109 bp, with a G + C content of 60.55%. Comparison of its open reading frames shows that the closest...

journal_title：FEMS microbiology letters

authors： Valdes N,Espinoza C,Sanhueza L,Gonzalez A,Corsini G,Tello M

更新日期：2015-03-01 00:00:00

abstract：:Xylose reductase catalyzes the NAD(P)H-dependent reduction of xylose to xylitol and is essential for growth on xylose by yeasts. To understand the nature of coenzyme binding to the Pichia stipitis xylose reductase, we investigated the role of the strictly conserved Lys270 in the putative IPKS coenzyme binding motif by...

journal_title：FEMS microbiology letters

authors： Kostrzynska M,Sopher CR,Lee H

更新日期：1998-02-01 00:00:00

abstract：:Different genes encoding pyridine nucleotide transhydrogenases (pntAB, udhA) and acetoacetyl-CoA reductases (phaB) were heterologously overexpressed in Rhodospirillum rubrum S1. A recombinant strain, which harbored the gene encoding the membrane-bound transhydrogenase PntAB from Escherichia coli MG1655 and the phaB1 g...

journal_title：FEMS microbiology letters

authors： Heinrich D,Raberg M,Steinbüchel A

更新日期：2015-04-01 00:00:00

abstract：:To rapidly identify natural isolates of marine bioluminescent bacteria, we developed amplified ribosomal DNA restriction analysis (ARDRA) methods. ARDRA, which is based on the restriction patterns of 16S rRNA gene digested with five enzymes (EcoRI, DdeI, HhaI, HinfI, RsaI), clearly distinguished the 14 species of mari...

journal_title：FEMS microbiology letters

authors： Kita-Tsukamoto K,Wada M,Yao K,Kamiya A,Yoshizawa S,Uchiyama N,Kogure K

更新日期：2006-03-01 00:00:00

abstract：:A recombinant lipase, CWB-LipB, localized on the Bacillus subtilis cell surface and retaining lipase activity was unstable and not accumulated in a high yield. To improve the accumulation, we examined cell wall binding protease (wprA)- and/or sigma D (sigD)-deficient mutants, and also a NprE and AprA protease-deficien...

journal_title：FEMS microbiology letters

authors： Kobayashi G,Toida J,Akamatsu T,Yamamoto H,Shida T,Sekiguchi J

更新日期：2000-07-15 00:00:00

abstract：:The production of candidate affinity proteins in a soluble form, for downstream characterization, is often a time-consuming step in combinatorial protein engineering methods. Here, a novel approach for efficient production of candidate clones is described based on direct cleavage of the affinity protein from the surfa...

journal_title：FEMS microbiology letters

authors： Kronqvist N,Löfblom J,Severa D,Ståhl S,Wernérus H

更新日期：2008-01-01 00:00:00