Bacillus subtilis YabA is involved in determining the timing and synchrony of replication initiation.

Abstract:

:It is shown here by flow cytometry that Bacillus subtilis YabA negatively regulates the timing of replication initiation. When the level of YabA was reduced, replication began at a decreased cell mass and when the level was increased, initiation was delayed. Synchrony of replication initiation was also disrupted at low levels of YabA. Yfp-YabA localized as foci in cells. Since YabA was reported to interact with DnaN (beta subunit of DNA polymerase III), co-localization of Yfp-YabA with the polymerase was examined using a Cfp fusion with DnaX (tau subunit of DNA polymerase III). It is reported that YabA appears to localize at the replication forks only at a late stage of DNA replication.

journal_name

FEMS Microbiol Lett

authors

Hayashi M,Ogura Y,Harry EJ,Ogasawara N,Moriya S

doi

10.1016/j.femsle.2005.04.028

keywords:

subject

Has Abstract

pub_date

2005-06-01 00:00:00

pages

73-9

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(05)00260-0

journal_volume

247

pub_type

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