Abstract:
:It is shown here by flow cytometry that Bacillus subtilis YabA negatively regulates the timing of replication initiation. When the level of YabA was reduced, replication began at a decreased cell mass and when the level was increased, initiation was delayed. Synchrony of replication initiation was also disrupted at low levels of YabA. Yfp-YabA localized as foci in cells. Since YabA was reported to interact with DnaN (beta subunit of DNA polymerase III), co-localization of Yfp-YabA with the polymerase was examined using a Cfp fusion with DnaX (tau subunit of DNA polymerase III). It is reported that YabA appears to localize at the replication forks only at a late stage of DNA replication.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Hayashi M,Ogura Y,Harry EJ,Ogasawara N,Moriya Sdoi
10.1016/j.femsle.2005.04.028keywords:
subject
Has Abstractpub_date
2005-06-01 00:00:00pages
73-9issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(05)00260-0journal_volume
247pub_type
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