Abstract:
:Xanthomonas oryzae pv. oryzae is an important plant pathogen which causes bacterial blight of rice. To facilitate genome studies of this bacterium, we have constructed a bacterial artificial chromosome (BAC) library of strain MAFF 311018. It consisted of 750 clones representing 16 genome equivalents, and had an insert size ranging from 20 to 220 kb with an average size of 107 kb. This library is the first to be constructed from a X. oryzae pv. oryzae strain. The usefulness of this library was demonstrated through polymerase chain reaction screening of 11 genes and the 16S--23S rDNA spacer region in a 192-clone subset, representing five genome equivalents. The results obtained showed an average of 5.9 BAC clones per screening. This result is in good agreement with the estimated size of the test library, indicating that the constructed BAC library can be used to facilitate genome analysis of X. oryzae pv. oryzae.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Ochiai H,Inoue Y,Hasebe A,Kaku Hdoi
10.1111/j.1574-6968.2001.tb10693.xkeywords:
subject
Has Abstractpub_date
2001-06-12 00:00:00pages
59-65issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(01)00194-Xjournal_volume
200pub_type
杂志文章abstract::Members of the genus Actinoplanes are considered to be representative of motile actinomycetes. To infer the flagellar diversity of Actinoplanes species, novel degenerate primers were designed for the flagellin (fliC) gene. The fliC gene of 21 Actinoplanes strains was successfully amplified and classified into two grou...
journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:1998-07-15 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:1995-04-15 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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pub_type: 杂志文章
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journal_title:FEMS microbiology letters
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更新日期:2015-03-01 00:00:00
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:1993-12-01 00:00:00
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