Increased expression of biodegradative threonine dehydratase of Escherichia coli by DNA gyrase inhibitors.

Abstract:

:The synthesis of inducible biodegradative threonine dehydratase of Escherichia coli increased several-fold in the presence of the DNA gyrase inhibitors, nalidixic acid and coumermycin. Temperature-sensitive gyrB mutants expressed higher levels of dehydratase as compared to an isogenic gyrB+ strain. Immunoblotting experiments showed increased synthesis of the dehydratase protein in the presence of gyrase inhibitors; addition of rifampicin and chloramphenicol to cells actively synthesizing enzyme preventing new enzyme production. Increased expression of dehydratase by gyrase inhibitors was accompanied by relaxation of supercoiled DNA.

journal_name

FEMS Microbiol Lett

authors

Sumantran VN,Tranguch AJ,Datta P

doi

10.1016/0378-1097(89)90362-5

subject

Has Abstract

pub_date

1989-11-01 00:00:00

pages

37-40

issue

1-2

eissn

0378-1097

issn

1574-6968

journal_volume

53

pub_type

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